Human Embryonic Stem Cells

by Chiu, Arlene Y.; Rao, Mahendra S.

• ISBN13: 9781588293114
• ISBN10: 1588293114
• Format: Hardcover
• Copyright: 2003-07-01
• Publisher: Humana Pr Inc

Summary

A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.

Table of Contents

Preface	v
Contributors	xvii
PART I: POLICY
1 Ethical Issues Associated with Pluripotent Stem Cells	3	(24)
Steve Usdin
2 A Researcher's Guide to Federally Funded Human Embryonic Stem Cell Research in the United States	27	(12)
Gregory J. Downing
3 Intellectual Property of Human Pluripotent Stem Cells	39	(24)
Mark L. Rohrbaugh
PART II: TYPES OF PLURIPOTENT CELLS
4 Embryonal Carcinoma Cells: The Malignant Counterparts of ES and EG Cells	63	(26)
Jonathan S. Draper, Harry Moore, and Peter W. Andrews
5 Human Pluripotent Stem Cells from Bone Marrow	89	(24)
Felipe Prosper and Catherine M. Verfaillie
6 Protocols for the Isolation and Maintenance of Human Embryonic Stem Cells	113	(14)
Melissa K. Carpenter, Chunhui Xu, Christine A. Daigh, Jessica E. Antosiewicz, and James A. Thomson
7 Subcloning and Alternative Methods for the Derivation and Culture of Human Embryonic Stem Cells	127	(18)
Michal Amit, Hanna Segev, Dorit Manor, and Joseph Itskovitz-Eldor
PART III: DIFFERENTIATION
8 Differentiation of Neuroepithelia from Human Embryonic Stem Cells	145	(16)
Su-Chun Zhang
9 Pancreatic Differentiation of Pluripotent Stem Cells	161	(20)
Nadya Lumelsky
10 Human Embryonic Stem Cell-Derived Cardiomyocyten: Derivation and Characterization	181	(20)
Chunhui Xu and Melissa K. Carpenter
11 Vascular Lineage Differentiation from Human Embryonic Stem Cells	201	(18)
Sharon Gerecht-Nir and Joseph Itskovitz-Eldor
12 Hematopoietic Progenitors Derived from Human Embryonic Stem Cells	219	(20)
Dan S. Kaufman
PART IV: THERAPEUTICS
13 Human Embryonic vs Adult Stem Cells for Transplantation Therapies	239	(26)
Calvin B. Harley and Mahendra S. Rao
14 Genetic Manipulation of Human Embryonic Stem Cells	265	(20)
Micha Drukker and Nissim Benvenisty
15 Human Therapeutic Cloning	285	(12)
Jose B. Cibelli
16 Therapeutic Uses of Embryonic Stem Cells	297	(26)
Alexander Kamb, Mani Ramaswami, and Mahendra S. Rao
17 Human Embryonic Stem Cells and the Food and Drug Administration: Assuring the Safety of Novel Cellular Therapies	323	(22)
Donald W. Fink, Jr.
18 Studies of a Human Neuron-Like Cell Line in Stroke and Spinal Cord Injury: Preclinical and Clinical Perspectives	345	(44)
Paul J. Reier, John Q. Trojanowski, Virginia M-Y. Lee, and Margaret J. Velardo
Appendix I: Cell Lines and Companies Involved with Human Embryonic Stem Cell Research	389	(4)
Appendix II: Useful Websites	393	(6)
Appendix III: Research Agreements and Material Transfer Agreements Between Investigator and Stem Cell Provider	399	(14)
Appendix IV: Stem Cell Patents	413	(38)
Index	451