Insect Transgenesis: Methods and Applications

by Handler; Alfred M.

• ISBN13: 9780849320286
• ISBN10: 0849320283
• Edition: 1st
• Format: Hardcover
• Copyright: 2000-06-28
• Publisher: CRC Press

Summary

Imagine scientists controlling the transmission of certain diseases through the genetic modification of mosquitoes. Eradicating harmful insects without the use of pesticides. Or increasing the fertility of some insects who in turn eat harmful arthropods or even a plant pathogen. Those are just a few of the real-world applications of insect transgenesis, which offers substantial benefits to humankind-whether it be in improving agricultural productivity or reducing the spread of insect-vectored diseases.Insect Transgenesis: Methods and Applications is the first publication to describe in a comprehensive manner the various methodologies available, possible applications, and the risk assessment and regulatory issues involved in this fascinating area of research.Divided into several areas of interest, the book starts with an overview of the history and methodology of insect gene transfer. The book then examines gene targeting by homologous recombination and recombination systems, and systems for transgenic selection, including visible eye color markers, chemical resistance, and fluorescent proteins. Other sections consider the use of various vector systems to integrate DNA into a host genome or to express foreign genes in a host organism.The work concludes with strategies for the use of transgenic insects, including examples for agricultural pests and vectors of disease. Of particular interest are the final chapters that discuss risk assessment considerations and governmental regulatory procedures for the transport and release of transgenic insects.

Table of Contents

SECTION I --- INTRODUCTION
An Introduction to the History and Methodology of Insect Gene Transfer	3	(26)
Alfred M. Handler
SECTION II --- GENE TARGETING
Targeted Transformation of the Insect Genome	29	(24)
Paul Eggleston
Yuguang Zhao
Site-Specific Recombination for the Genetic Manipulation of Transgenic Insects	53	(26)
Yikang S. Rong
Kent G. Golic
SECTION III --- TRANSGENIC SELECTION
Eye Color Genes for Selection of Transgenic Insects	79	(14)
Abhimanyu Sarkar
Frank H. Collins
Green Fluorescent Protein (GFP) as a Marker for Transgenic Insects	93	(16)
Stephen Higgs
David L. Lewis
Resistance Genes as Candidates for Insect Transgenesis	109	(16)
Richard H. ffrench-Constant
Mark Q. Benedict
SECTION IV --- VIRAL VECTORS
Pantropic Retroviral Vectors for Insect Gene Transfer	125	(14)
Jane C. Burns
Densonucleosis Viruses as Transducing Vectors for Insects	139	(22)
Jonathan Carlson
Boris Afanasiev
Erica Suchman
Sindbis Virus Expression System in Mosquitoes: Background, Methods, and Applications	161	(30)
Ken E. Olson
Retrotransposons and Retroviruses in Insect Genomes	191	(12)
Christophe Terzian
Alain Pelisson
Alain Bucheton
Polydnaviruses and Insect Transgenic Research	203	(16)
Bruce A. Webb
SECTION V --- TRANSPOSABLE ELEMENT VECTORS
Hermes and Other hat Elements as Gene Vectors in Insects	219	(18)
Peter W. Atkinson
David A. O'Brochta
Genetic Engineering of Insects with mariner Transposons	237	(12)
David J. Lampe
Kimberly K. O. Walden
John M. Sherwood
Hugh M. Robertson
The TTAA-Specific Family of Transposable Elements: Identification, Functional Characterization, and Utility for Transformation of Insects	249	(22)
Malcolm J. Fraser, Jr.
SECTION VI --- SYMBIONT VECTORS
Wolbachia as a Vehicle to Modify Insect Populations	271	(18)
Steven P. Sinkins
Scott L. O'Neill
Bacterial Symbiont Transformation in Chagas Disease Vectors	289	(18)
Charles B. Beard
Ravi V. Durvasula
Frank F. Richards
SECTION VII --- STRATEGIES, RISK ASSESSMENT, AND REGULATION
The Application of Transgenic Insect Technology in the Sterile Insect Technique	307	(12)
Alan S. Robinson
Gerald Franz
Control of Disease Transmission through Genetic Modification of Mosquitoes	319	(16)
Anthony A. James
Deploying Transgenic Arthropods in Pest Management Programs: Risks and Realities	335	(34)
Marjorie A. Hoy
Regulation of Transgenic Arthopods and Other Invertebrates in the United States	369	(12)
Orrey P. Young
Shirley P. Ingebritsen
Arnold S. Foudin
Index	381