With its distinctive investigative approach to learning, this best-selling laboratory manual is now more engaging than ever, with full-color art and photos throughout. The lab manual encourages students to participate in the process of science and develop creative and critical-reasoning skills.

1 Scientific Investigation

Laboratory Objectives

Introduction

Exercise 1.1 Questions and Hypotheses

Exercise 1.2 Designing Experiments to Test Hypotheses

Exercise 1.3 Designing an Experiment

Exercise 1.4 Presenting and Analyzing Results

Exercise 1.5 Interpreting and Communicating Results

Reviewing Your Knowledge

Applying Your Knowledge

Student Media: BioFlix, Activities, Investigations, Videos, and Data Tables

References

Websites

2 Microscopes and Cells

Laboratory Objectives

Introduction

Exercise 2.1 The Compound Light Microscope

Exercise 2.2 Basic Microscope Techniques

Exercise 2.3 The Stereoscopic Microscope

Exercise 2.4 The Transmission Electron Microscope

Exercise 2.5 The Organization of Cells

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

3 Diffusion and Osmosis

Laboratory Objectives

Introduction

Exercise 3.1 Diffusion of Molecules

Exercise 3.2 Osmotic Activity in Cells

Exercise 3.3 Investigating 0smolarity of Plant Cells

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions

Student Media: BioFlix, Activities, Investigations, Videos, and Data Tables

References

Websites

4 Enzymes

Laboratory Objectives

Introduction

Exercise 4.1 Experimental Method and the Action of Catechol 0xidase 90 Exercise 4.2 Inhibiting the Action of Catechol Oxidase 93

Exercise 4.3 Influence of Enzyme Concentration, pH, and Temperature on the Activity of Amylase

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions

Student Media: BioFlix, Activities, Investigations, Videos, and Data Tables

References

Websites

5 Cellular Respiration and Fermentation

Laboratory Objectives

Introduction

Exercise 5.1 Alcoholic Fermentation

Exercise 5.2 Cellular Respiration

Exercise 5.3 Designing and Performing Your Open-Inquiry Investigation

Reviewing Your Knowledge

Applying Your Knowledge

Student Media: BioFlix, Activities, Investigations, Videos, and Data Tables

References

Websites

6 Photosynthesis

Laboratory Objectives

Introduction

Exercise 6.1 The Wavelengths of Light for Photosynthesis

Exercise 6.2 Pigments in Photosynthesis

Exercise 6.3 Separation and Identification of Plant Pigments by Paper Chromatography

Exercise 6.4 Determining the Absorption Spectrum for Leaf Pigments

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions

Student Media: BioFlix, Activities, Investigations, Videos, and Data Tables

References

Websites

7 Mitosis and Meiosis

Laboratory Objectives

Introduction

Exercise 7 .1 Modeling the Cell Cycle and Mitosis in an Animal Cell

Exercise 7.2 Observing Mitosis and Cytokinesis in Plant Cells

Exercise 7.3 Observing Chromosomes, Mitosis, and Cytokinesis in Animal Cells

Exercise 7.4 Modeling Meiosis

Exercise 7 .5 Meiosis in Sordaria fimicola: A Study of Crossing Over

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

8 Mendelian Genetics I: Fast Plants

Laboratory Objectives

Introduction

Exercise 8.1 Inheritance of Anthocyanin Gene

Exercise 8.2 Inheritance of Plant Color: Green, Yellow-Green, and Purple (Anthocyanin Present)

Exercise 8.3 Inheritance of Variegated and Nonvariegated Leaf Color

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions 214

Student Media: BioFlix, Activities, Investigations, Videos, and Data Tables

References

Websites

9 Mendelian Genetics II: Drosophila

Laboratory Objectives

Introduction

Exercise 9.1 Establishing the Enzyme Reaction Controls

Exercise 9.2 Determining the Pattern of Inheritance of the Aldox Gene

Exercise 9.3 Determining Parental Genotypes Using Evidence from Progeny

Exercise 9.4 Mapping Genes

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions

Student Media: BioFlix, Activities, Investigations, Videos, and Data Tables

References

Websites

10 Molecular Biology

Laboratory Objectives

Introduction

Exercise 10.1 Mapping DNA Using Restriction Enzymes and Electrophoresis

Exercise 10.2 Practice Problem for Mapping DNA

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

11 Population Genetics: The Hardy-Weinberg Equilibrium

Laboratory Objectives

Introduction

Exercise 11.1 Testing Hardy-Weinberg EqÀ¿iliÀ¿tium Using a Bead Model

Exercise 11.2 Simulation of Evolutionary Change Using the Bead Model

Exercise 11.3 Open-Inquiry Computer Simulation of Evolution

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions

Student Media: BioFlix, Activities, Investigations, Videos, and Data Tables

References

Websites

12 Bacteriology

Laboratory Objectives

Introduction

Exercise 12.1 Investigating Characteristics of Bacteria

Exercise 12.2 Ecological Succession of Bacteria in Milk

Exercise 12.3 Bacteria in the Environment

Exercise 12.4 Controlling the Growth of Bacteria

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

13 Protists

Laboratory Objectives

Introduction

Exercise 13.1 Supergroup Excavata

Exercise 13.2 Supergroup •sAR•

Exercise 13.3 Supergroup Unikonta

Exercise 13.4 Supergroup Archaeplastida

Exercise 13.5 Designing and Performing an Open-Inquiry Investigation

Reviewing Your Knowleqge

Applying Your Knowledge

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

14 Plant Diversity I: Bryophytes (Nonvascular Plants) and Seedless Vascular Plants

Laboratory Objectives

Introduction

Exercise 14.1 Bryophytes (Nonvascular Plants)

Exercise 14.2 Seedless Vascular Plants

Reviewing Your Knowledge

Applying Your Knowledge

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

15 Plant Diversity II: Seed Plants

Laboratory Objectives

Exercise 15.1 Gymnosperms

Exercise 15.2 Angiosperms

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extension

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

16 Bioinformatics: Molecular Phylogeny of Plants

Exercise 16.1 Establishing Molecular Phylogenetic Relationships Among Rubisco Large Subunit Genes

Exercise 16.2 Analyzing Phylogenetic Trees and Reporting Results

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extension

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

17 The Kingdom Fungi

Laboratory Objectives

Introduction

Exercise 17.1 A Survey of Major Fungal Groups

Exercise 17.2 Designing and Performing an Open-Inquiry Investigation

Reviewing Your Knowledge

Applying Your Knowledge

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

18 Animal Diversity I: Porifera, Cnidaria, Platyhelminthes, Mollusca, and Annelida

Laboratory Objectives

Introduction

Exercise 18.1 Phylum Porifera- Sponges(Scypha)

Exercise 18.2 Phylum Cnidaria-Hydras (Hydra)

Exercise 18.3 Phylum Pla tyhelminthes- Planarians(Dugesia)

Exercise 18.4 Phylum Mollusca-Clams

Exercise 18.5 Phylum Annelida-Clamworms (Nereis) and Earthworms (Lumbricus terrestris)

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extension

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

19 Animal Diversity II: Nematoda, Arthropoda, Echinodermata, and Chordata

Exercise 19.1 Phylum Nematoda-Roundworms (Ascaris)

Exercise 19.2 Phylum Arthropoda

Exercise 19.3 Phylum Echinodermata- Sea Star

Exercise 19.4 Phylum Chordata

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extension

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

20 Plant Anatomy

Laboratory Objectives

Introduction

Exercise 20.1 Plant Morphology

Exercise 20.2 Plant Primary Growth and Development

Exercise 20.3 Cell Structure of Primary Tissues

Exercise 20.4 Structure of Tissues Produced by Secondary Growth

Exercise 20.5 Grocery Store Botany: Modifications of Plant Organs

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extension

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

21 Plant Growth

Laboratory Objectives

Introduction

Exercise 21.1 Factors Influencing Seed Germination

Exercise 21.2 Plant Growth Regulators: Auxin

Exercise 21.3 Plant Growth Regulators: Gibberellins

Exercise 21.4 Designing and Performing an Open-Inquiry Investigation

Reviewing Your Knowledge

Applying Your Knowledge

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

22 Vertebrate Anatomy I: The Skin and Digestive System

Laboratory Objectives

Introduction

Exercise 22.1 Histology of the Skin

Exercise 22.2 Introduction to the Fetal Pig

Exercise 22.3 The Digest ive System in the Fetal Pig

Reviewing Your Knowledge

Applying Your Knowledge

Case Study

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

23 Vertebrate Anatomy II: The Circulatory and Respiratory Systems

Laboratory Objectives

Introduction

Exercise 23.1 Glands and Respiratory Structures of the Neck and Thoracic Cavity

Exercise 23.2 The Heart and the Pulmonary Blood Circuit

Exercise 23.3 The Heart and the Systemic Circuit in the Thorax

Exercise 23.4 The Systemic Circuit in the Abdominal Cavity

Exercise 23.5 Blood

Exercise 23.6 Fetal Pig Circulation

Exercise 23.7 Details of the Respiratory System

Reviewing Your Knowledge

Applying Your Knowledge

Case Studies

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

24 Vertebrate Anatomy Ill: The Excretory Reproductive, and Nervous Systems

Laboratory Objectives

Introduction

Exercise 24.1 The Excretory System

Exercise 24.2 The Reproductive System

Exercise 24.3 Nervous Tissue, the Spinal Cord and Reflex Arc, the Sheep Brain, and the Vertebrate Eye

Reviewing Your Knowledge

Applying Your Knowledge

Case Studies

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

25 Animal Development

Laboratory Objectives

Introduction

Exercise 25.1 Development in Echinoderms: Sea Urchin and Sea Star

Exercise 25.2 Development in an Amphibian

Exercise 25.3 Development in the Zebraflsh

Exercise 25.4 Development in a Bird: The Chicken

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

26 Animal Behavior

Laboratory Objectives

Introduction

Exercise 26.1 Taxis in Brine Shrimp

Exercise 26.2 Kinesis in Pill Bugs

Exercise 26.3 Agonistic Display in Male Siamese Fighting Fish

Exercise 26.4 Reproductive Behavior in Fruit Flies

Exercise 26.5 Designing and Performing Your Open-Inquiry Investigation

Reviewing Your Knowledge

Applying Your Knowledge

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

27 Ecology I: Terrestrial Eco logy

Laboratory Objectives

Introduction

Exercise 27.1 Biotic Components

Exercise 27.2 Ablotic Components

Exercise 27.3 Data Analysis

Reviewing Your Knowledge

Applying Your Knowledge

Optional Exercise: Investigating Weedy Fields

Student Media: BioFlix, Activities, Investigations, Videos, and Data Tables

References

Websites

28 Ecology II: Computer Simulations of a Pond Ecosystem

Laboratory Objectives

Introduction

Exercise 28.1 Computer Model of the Pond Ecosystem

Exercise 28.2 Investigating the Effects of Disturbance on the Pond Ecosystem

Reviewing Your Knowledge

Applying Your Knowledge

Investigative Extensions

Student Media: BioFlix, Activities, Investigations, and Videos

References

Websites

Appendix A Scientific Writing and Communication

Appendix B The Metric System

Appendix C Instrumentation and Techniques

Appendix D Chi-Square Test

Appendix C Terminology and Techniques for Dissection

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