Isolation and Purification of Proteins

by ;
  • ISBN13:


  • ISBN10:


  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2003-02-05
  • Publisher: CRC Press

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

Purchase Benefits

  • Free Shipping On Orders Over $35!
    Your order must be $35 or more to qualify for free economy shipping. Bulk sales, PO's, Marketplace items, eBooks and apparel do not qualify for this offer.
  • Get Rewarded for Ordering Your Textbooks! Enroll Now
List Price: $335.00 Save up to $174.20
  • Rent Book $284.75
    Add to Cart Free Shipping


Supplemental Materials

What is included with this book?

  • The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.
  • The Rental and eBook copies of this book are not guaranteed to include any supplemental materials. Typically, only the book itself is included. This is true even if the title states it includes any access cards, study guides, lab manuals, CDs, etc.


This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.

Table of Contents

Prefacep. iii
Contributorsp. vii
Introductionp. ix
Release of Protein from Biological Hostp. 1
Chromatography: The High-Resolution Technique for Protein Separationp. 29
Selectivity in Affinity Chromatographyp. 57
Genetic Approaches to Facilitate Protein Purificationp. 95
Solid-Liquid Separationp. 131
Membranes for Protein Isolation and Purificationp. 191
Precipitation of Proteins: Nonspecific and Specificp. 225
Protein Crystallization for Large-Scale Bioseparationp. 277
Extraction for Rapid Protein Isolationp. 321
Adsorption as an Initial Step for the Capture of Proteinsp. 373
Fast Chromatography of Proteinsp. 423
Novel Approaches to the Chromatography of Proteinsp. 455
Scale-up and Commercial Production of Recombinant Proteinsp. 503
Process Monitoring During Protein Purificationp. 515
Protein Formulationp. 549
Regulatory Requirements, Cleaning, and Sanitization Issues in Protein Purificationp. 605
Indexp. 623
Table of Contents provided by Syndetics. All Rights Reserved.

Rewards Program

Write a Review