Laboratory Applications in Microbiology: A Case Study Approach

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  • Edition: 1st
  • Format: Spiral Bound
  • Copyright: 2008-09-17
  • Publisher: McGraw-Hill Science/Engineering/Math
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Laboratory Applications in Microbiology: A Case Study Approachuses real-life case studies as the basis for exercises in the laboratory. This is the only microbiology lab manual focusing on this means of instruction, an approach particularly applicable to the microbiology laboratory. The author has carefully organized the exercises so that students develop a solid intellectual base beginning with a particular technique, moving through the case study, and finally applying new knowledge to unique situations beyond the case study.

Table of Contents

Essential Laboratory Skills
Safety Considerations in the Microbiology Laboratory CASE STUDIES Laboratory-Acquired Infection with Escherichia coli O157:H7—New York, 2004 Laboratory-Acquired Brucellosis—New York, 2001Ocular Vaccinia Infection of a Laboratory Worker—Philadelphia, 2004
Microscopy and Measurement of Microscopic Specimens CASE STUDY Excerpt of Letters from Anton van Leeuwenhoek to the Royal Society of London for the Improvement of Natural Knowledge Survey Of Microorganisms
Identification and Classification of Algae CASE STUDY Oregon Harmful Bloom Monitoring Project
Survey of Medically Important Protozoa CASE STUDY Acanthamoeba Keratitis—Multiple States
Ubiquity of Bacteria CASE STUDY Engineering Infection Control through Facility Design
Identification and Classification of Fungi CASE STUDY Outbreak of Acute Febrile Respiratory Illness among College Students—Acapulco, Mexico, March 2001 Manipulation, Staining, And Observation Of Microorganisms
Aseptic Techniques CASE STUDY Multiple Misdiagnoses of Tuberculosis Resulting from Laboratory Error—Wisconsin, 1996
Pure Culture Techniques CASE STUDY Neonatal Tetanus—Montana, 1998
Simple Staining, Negative Staining, and Gram Staining CASE STUDY Identification of Bacteria Responsible for the Outbreak of Gastrointestinal Disease
Capsular Staining CASE STUDY Pneumococcal Sepsis after Autosplenectomy in a Girl with Systemic Lupus Erythematosus—2005
Acid-Fast and Endospore Staining CASE STUDIES Mycobacterium tuberculosis Transmission in a Newborn Nursery and Maternity Ward—New York City, 2003Inhalation Anthrax Associated with Dried Animal Hides—Pennsylvania and New York City, 2006 Environmental Influences On The Growth Of Microorganisms
Viable Plate Count CASE STUDY Multidrug-Resistant Salmonella Typhimurium Infection from Milk Contaminated after Pasteurization
Cultivation of Anaerobes CASE STUDY Botulism from Home-Canned Bamboo Shoots—Nan Province, Thailand, March 2006
Temperature Effects on Bacterial Growth and Survival CASE STUDY Outbreak of Gastroenteritis Associated with Consumption of Alaskan Oysters
pH and Microbial Growth CASE STUDY Garlic-in-Oil-Associated Botulism: Episode Leads to Product Modification
Effects of Osmotic Pressure on Bacterial Growth CASE STUDY Outbreak of Staphylococcal Food Poisoning Associated with Precooked Ham—Florida, 1997 Control Of Microbial Growth
Lethal Effects of Ultraviolet Light CASE STUDY Gastrointestinal Outbreak Traced to Interactive Fountain—New York, March 2006
Evaluation of Disinfectants: Use-Dilution Method CASE STUDY Nine Episodes of Anaphylaxis Following Cystoscopy Caused by a High-Level Disinfectant—2004
Effectiveness of Hand Scrubbing CASE STUDY Puerperal Fever—Vienna, Austria, 1847
Antimicrobic Sensitivity Testing: Kirby-Bauer, Tube Dilution, and E-Test Methods CASE STUDY Methicillin-Resistant Staphylococcus aureus Infections among Tattoo Recipients—Ohio, Kentucky, and Vermont, 2004–2005 Epidemiology
Phage Typing of Bacteria CASE STUDY Salmonella Serotype Enteritidis Infections among Workers Producing Poultry Vaccine—Maine, November–December, 2006
Simulated Epidemic CASE STUDY Import-Associated Measles Outbreak—Indiana, May–June 2005
Morbidity and Mortality Weekly Report CASE STUDY Multistate Outbreak of Mumps—United States, January 1–October 7, 2006 Microbial Genetics
Bacterial Transformation CASE STUDY Staphylococcus aureus Resistant to Vancomycin—United States, 2002
The Ames Test CASE STUDY West Nile Virus Update—United States, January 1–November 13, 2007 Applied Microbiology
DNA Extraction from Bacterial Cells CASE STUDY Multistate Outbreaks of Salmonella Infections Associated with Raw Tomatoes Eaten in Restaurants—United States, 2005–2006
Measures of Water Quality: Most Probable Number Procedure CASE STUDY E. coli Contamination of Water Supply—Frazier Park, California, 2007
Measures of Water Quality: Membrane Filtration Method CASE STUDY Fecal Contamination of Airline Drinking Water—2005
Measures of Milk Quality: Methylene Blue Reductase Test CASE STUDY Salmonella Typhimurium Infection Associated with Raw Milk and Cheeses Consumption—Pensylvania, 2007
Bacterial Counts of Food CASE STUDY Multiple Outbreaks of Food Poisoning—1989–2006 Medical Microbiology
Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacteriaceae CASE STUDY Outbreaks of Escherichia coli O157:H7 Associated with Petting Zoos—North Carolina, Florida, and Arizona, 2004 and 2005
Isolation and Identification of Staphylococci CASE STUDY Community-Associated Methicillin-Resistant Staphylococcus aureus Infection among Healthy Newborns—Chicago and Los Angeles County, 2004
Isolation and Identification of Streptococci CASE STUDY Invasive Streptococcus pyogenes after Allograft Implantation—Colorado, 2003 Identification Of Unknown Bacteria
Identification of Bacterial Unknowns CASE STUDY Respiratory Disease Strikes Legionnaires Convention—Philadelphia, 1976 Laboratory Techniques, Reagents, And AssaysAnalysis of Bacterial Cultures Based on Morphological Characteristics
Colony Morphology
Growth in Solid and Liquid Media Staining Techniques Used for the Microscopic Examination of Bacteria
Simple Stain
Negative Stain
Capsule Stain
Gram Stain
Endospore Stain
Acid-Fast Stain
Motility Methods: Wet Mount and Hanging Drop
Flagella Stain Techniques for Inoculation of Media
Streak Plate Isolation
Loop Dilution
Spread Plate Commonly Used Differential and Selective Media
Fluid Thioglycollate Media
Mannitol Salt Agar
MacConkey Agar
Desoxycholate Agar
Endo Agar
Eosin Methylene Blue Agar
Hektoen Enteric Agar
Xylose Lysine Desoxycholate Agar
Blood Agar
Motility Media
SIM Medium
Kligler’s Iron Agar
Triple Sugar Iron Agar
Lysine Iron Agar
Litmus Milk Commonly Used Biochemical Tests
Oxidation-Fermentation Test
Phenol Red Broth
Purple Broth
Methyl Red and Voges-Proskauer Tests
Catalase Test
Oxidase Test
Nitrate Reduction Test
Coagulase Test
Citrate Test
Malonate Test
Decarboxylation Test
Phenylalanine Deaminase Test
Bile Esculin Test
Starch Hydrolysis
Urease Test
Casease Test
Gelatinase Test
DNase Test
Lipase Test
PYR Test Com
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