Laboratory Applications in Microbiology: A Case Study Approach

by Chess, Barry

• ISBN13: 9780073375250
• ISBN10: 007337525X
• Edition: 1st
• Format: Spiral Bound
• Copyright: 2008-09-17
• Publisher: McGraw-Hill Science/Engineering/Math

Summary

Laboratory Applications in Microbiology: A Case Study Approachuses real-life case studies as the basis for exercises in the laboratory. This is the only microbiology lab manual focusing on this means of instruction, an approach particularly applicable to the microbiology laboratory. The author has carefully organized the exercises so that students develop a solid intellectual base beginning with a particular technique, moving through the case study, and finally applying new knowledge to unique situations beyond the case study.

Table of Contents

Essential Laboratory Skills

Safety Considerations in the Microbiology Laboratory CASE STUDIES Laboratory-Acquired Infection with Escherichia coli O157:H7—New York, 2004 Laboratory-Acquired Brucellosis—New York, 2001Ocular Vaccinia Infection of a Laboratory Worker—Philadelphia, 2004

Microscopy and Measurement of Microscopic Specimens CASE STUDY Excerpt of Letters from Anton van Leeuwenhoek to the Royal Society of London for the Improvement of Natural Knowledge Survey Of Microorganisms

Identification and Classification of Algae CASE STUDY Oregon Harmful Bloom Monitoring Project

Survey of Medically Important Protozoa CASE STUDY Acanthamoeba Keratitis—Multiple States

Ubiquity of Bacteria CASE STUDY Engineering Infection Control through Facility Design

Identification and Classification of Fungi CASE STUDY Outbreak of Acute Febrile Respiratory Illness among College Students—Acapulco, Mexico, March 2001 Manipulation, Staining, And Observation Of Microorganisms

Aseptic Techniques CASE STUDY Multiple Misdiagnoses of Tuberculosis Resulting from Laboratory Error—Wisconsin, 1996

Pure Culture Techniques CASE STUDY Neonatal Tetanus—Montana, 1998

Simple Staining, Negative Staining, and Gram Staining CASE STUDY Identification of Bacteria Responsible for the Outbreak of Gastrointestinal Disease

Capsular Staining CASE STUDY Pneumococcal Sepsis after Autosplenectomy in a Girl with Systemic Lupus Erythematosus—2005

Acid-Fast and Endospore Staining CASE STUDIES Mycobacterium tuberculosis Transmission in a Newborn Nursery and Maternity Ward—New York City, 2003Inhalation Anthrax Associated with Dried Animal Hides—Pennsylvania and New York City, 2006 Environmental Influences On The Growth Of Microorganisms

Viable Plate Count CASE STUDY Multidrug-Resistant Salmonella Typhimurium Infection from Milk Contaminated after Pasteurization

Cultivation of Anaerobes CASE STUDY Botulism from Home-Canned Bamboo Shoots—Nan Province, Thailand, March 2006

Temperature Effects on Bacterial Growth and Survival CASE STUDY Outbreak of Gastroenteritis Associated with Consumption of Alaskan Oysters

pH and Microbial Growth CASE STUDY Garlic-in-Oil-Associated Botulism: Episode Leads to Product Modification

Effects of Osmotic Pressure on Bacterial Growth CASE STUDY Outbreak of Staphylococcal Food Poisoning Associated with Precooked Ham—Florida, 1997 Control Of Microbial Growth

Lethal Effects of Ultraviolet Light CASE STUDY Gastrointestinal Outbreak Traced to Interactive Fountain—New York, March 2006

Evaluation of Disinfectants: Use-Dilution Method CASE STUDY Nine Episodes of Anaphylaxis Following Cystoscopy Caused by a High-Level Disinfectant—2004

Effectiveness of Hand Scrubbing CASE STUDY Puerperal Fever—Vienna, Austria, 1847

Antimicrobic Sensitivity Testing: Kirby-Bauer, Tube Dilution, and E-Test Methods CASE STUDY Methicillin-Resistant Staphylococcus aureus Infections among Tattoo Recipients—Ohio, Kentucky, and Vermont, 2004–2005 Epidemiology

Phage Typing of Bacteria CASE STUDY Salmonella Serotype Enteritidis Infections among Workers Producing Poultry Vaccine—Maine, November–December, 2006

Simulated Epidemic CASE STUDY Import-Associated Measles Outbreak—Indiana, May–June 2005

Morbidity and Mortality Weekly Report CASE STUDY Multistate Outbreak of Mumps—United States, January 1–October 7, 2006 Microbial Genetics

Bacterial Transformation CASE STUDY Staphylococcus aureus Resistant to Vancomycin—United States, 2002

The Ames Test CASE STUDY West Nile Virus Update—United States, January 1–November 13, 2007 Applied Microbiology

DNA Extraction from Bacterial Cells CASE STUDY Multistate Outbreaks of Salmonella Infections Associated with Raw Tomatoes Eaten in Restaurants—United States, 2005–2006

Measures of Water Quality: Most Probable Number Procedure CASE STUDY E. coli Contamination of Water Supply—Frazier Park, California, 2007

Measures of Water Quality: Membrane Filtration Method CASE STUDY Fecal Contamination of Airline Drinking Water—2005

Measures of Milk Quality: Methylene Blue Reductase Test CASE STUDY Salmonella Typhimurium Infection Associated with Raw Milk and Cheeses Consumption—Pensylvania, 2007

Bacterial Counts of Food CASE STUDY Multiple Outbreaks of Food Poisoning—1989–2006 Medical Microbiology

Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacteriaceae CASE STUDY Outbreaks of Escherichia coli O157:H7 Associated with Petting Zoos—North Carolina, Florida, and Arizona, 2004 and 2005

Isolation and Identification of Staphylococci CASE STUDY Community-Associated Methicillin-Resistant Staphylococcus aureus Infection among Healthy Newborns—Chicago and Los Angeles County, 2004

Isolation and Identification of Streptococci CASE STUDY Invasive Streptococcus pyogenes after Allograft Implantation—Colorado, 2003 Identification Of Unknown Bacteria

Identification of Bacterial Unknowns CASE STUDY Respiratory Disease Strikes Legionnaires Convention—Philadelphia, 1976 Laboratory Techniques, Reagents, And AssaysAnalysis of Bacterial Cultures Based on Morphological Characteristics

Colony Morphology

Growth in Solid and Liquid Media Staining Techniques Used for the Microscopic Examination of Bacteria

Simple Stain

Negative Stain

Capsule Stain

Gram Stain

Endospore Stain

Acid-Fast Stain

Motility Methods: Wet Mount and Hanging Drop

Flagella Stain Techniques for Inoculation of Media

Streak Plate Isolation

Loop Dilution

Spread Plate Commonly Used Differential and Selective Media

Fluid Thioglycollate Media

Mannitol Salt Agar

MacConkey Agar

Desoxycholate Agar

Endo Agar

Eosin Methylene Blue Agar

Hektoen Enteric Agar

Xylose Lysine Desoxycholate Agar

Blood Agar

Motility Media

SIM Medium

Kligler’s Iron Agar

Triple Sugar Iron Agar

Lysine Iron Agar

Litmus Milk Commonly Used Biochemical Tests

Oxidation-Fermentation Test

Phenol Red Broth

Purple Broth

Methyl Red and Voges-Proskauer Tests

Catalase Test

Oxidase Test

Nitrate Reduction Test

Coagulase Test

Citrate Test

Malonate Test

Decarboxylation Test

Phenylalanine Deaminase Test

Bile Esculin Test

Starch Hydrolysis

ONPG Test

Urease Test

Casease Test

Gelatinase Test

DNase Test

Lipase Test

CAMP Test

PYR Test Com

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