Laboratory Exercises in Microbiology, 2nd Edition

by Robert A. Pollack (Nassau Community College); Lorraine Findlay (Nassau Community College); Walter Mondschein (Nassau Community College); R. Ronald Modesto (C. W. Post campus of Long Island University)

• ISBN13: 9780471420828
• ISBN10: 0471420824
• Edition: 2nd
• Format: Paperback
• Copyright: 2004-10-01
• Publisher: Wiley

Summary

"The best feature of this lab manual is its style and organization. It is not just readable, it is thorough.... It is comparable to the best lab manuals I have used or read." - William Coleman, University of Hartford "It is a friendly-to-student book and I think student users will appreciate it--even if they don\'t realize why. I think they will like microbiology just a bit more." -Loyd J. Hays, University of Texas at San Antonio Clear, concise, and always engaging! Written specifically for allied health students, Pollack, Findlay, Mondschein, and Modesto's Laboratory Exercises in Microbiology, 2/e focuses on what students need to succeed in the lab: clear, concise, and highly engaging exercises and activities, rather than content that is simply adapted from a standard microbiology textbook. Throughout the manual, a variety of interactive activities and experiments convey the basic concepts of microbiology--all in a student-friendly tone. Students also have the opportunity to explore methods that allow the safe movement or transfer of microbial cells from one type of growth environment to another, classification and identification of microbes, microbial biochemistry, as well as laboratory exercises from medical, food, and environmental microbiology.

Table of Contents

Preface	iii
Introduction: Laboratory Operations and Safety: Hand Washing	vii
PART I GENERAL MICROSCOPY AND ASEPTIC TECHNIQUE	1	(30)
Laboratory Safety: Introduction to the Microscope	3	(14)
Transfer and Isolation Techniques	17	(14)
Microbes in the Environment	25	(6)
PART II MICROBIAL MORPHOLOGY, DIFFERENTIAL STAINS	31	(60)
Cultural and Cellular Morphology	33	(10)
Bacterial Growth	43	(8)
Gram Stain	51	(8)
Acid-Fast Stain	53	(6)
Endospore Stain	59	(8)
Capsule Stain	61	(1)
Hanging Drop Technique	62	(5)
Fungi	67	(8)
Viruses---Visualization and Enumeration	75	(6)
Parasitology	81	(10)
PART III MICROBIAL CONTROL AND BIOCHEMISTRY	91	(42)
Microbial Sensitivity Testing: Ultraviolet Light Sensitivity	93	(14)
Heat Sensitivity	96	(2)
Chemical Sensitivity	98	(2)
Antibiotic Sensitivity: Kirby-Bauer Technique	100	(7)
Bacterial Biochemistry	107	(10)
Gas Requirements of Microorganisms	117	(6)
Specialized Media	123	(10)
PART IV MEDICAL MICROBIOLOGY	133	(78)
Genetics	135	(6)
Epidemiology	141	(8)
Speciman-Handling Protocols	149	(12)
Specific Laboratory Tests	161	(8)
Serology	169	(10)
Identification of Enteric Pathogens: Traditional Methods	179	(8)
Identification of Enteric Pathogens: Rapid Identification Methods	187	(10)
Identification of a Bacterial Unknown: The Gram-Negative Unknown	197	(8)
Identification of a Bacterial Unknown: The Gram-Positive Cocci	205	(6)
PART V FOOD AND ENVIRONMENTAL MICROBIOLOGY	211	(32)
Identification and Quantification of Microbial Numbers in a Water Sample	213	(6)
Identification of Microbes in Beef and Poultry and the Quantification of Microbial Numbers	219	(6)
Soil Microbiology	225	(6)
Photographic Atlas	1	(230)
Microbial Ecology	231	(6)
Biofilms	237	(6)
Bibliography	243	(2)
Appendix 1	245	(5)
Appendix 2	250	(1)
Appendix 3	251	(1)
Credits	252	(1)
Index	253