Laboratory Experiments in Microbiology

by Johnson, Ted R.; Case, Christine L.

• ISBN13: 9780805376739
• ISBN10: 0805376739
• Edition: 7th
• Format: Spiral Bound
• Copyright: 2004-01-01
• Publisher: Benjamin Cummings

Summary

Newly revised to accompany Microbiology: An Introduction , Eighth Edition by Tortora, Funke, and Case, this lab manual includes 57 experiments that demonstrate the broad spectrum of microbiology. Intended as a manual of basic microbiological techniques, this popular lab manual features applications for undergraduate students in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. Experiments have been refined in this new edition to encourage students to develop critical-thinking skills as well as learn basic facts and technical skills. Material with direct application to clinical and commercial labs is included wherever possible, and increased emphasis is placed on lab safety.

Table of Contents

Introduction

Microscopy

Use and Care of the Microscope

Examination of Living Microorganisms

Staining Methods

Preparation of Smears and Simple Staining

Negative Staining

Gram Staining

Acid-fast Staining

Structural Stains (Endospore, Capsule, Flagella)

Morphologic Unknown

Cultivation of Bacteria

Microbes in the Environment

Transfer of Bacteria: Aseptic Techniques

Isolation of Bacteria by Dilution Technique

Special Media for Isolating Bacteria

Microbial Metabolism

Carbohydrate Catabolism

Fermentation of Carbohydrates

Protein Catabolism, Part 1

Protein Catabolism, Part 2

Respiration

Rapid Identification Methods

Microbial Growth

Oxygen and the Growth of Bacteria

Determination of a Bacterial Growth Curve: The Role of Temperature

Other Influences on Microbial Growth: Osmotic Pressure and pH

Control of Microbial Growth

Physical Methods of Control: Heat

Physical Methods of Control: Ultraviolet Radiation

Chemical Methods of Control: Disinfectants and Antiseptics

Chemical Methods of Control: Antimicrobial Drugs

Effectiveness of Hand Scrubbing

Microbial Genetics

Regulation of Gene Expression

Isolation of Bacterial Mutants

Isolation of DNA

DNA Fingerprinting

Transformation and Genetic Engineering

Ames Test for Detecting Possible Chemical Carcinogens

The Microbial World

Unknown Identification andBergey's Manual

Fungi: Yeasts

Fungi: Molds

Phototrophs: Algae and Cyanobacteria

Protozoa

Viruses

Isolation and Titration of Bacteriophages

Plant Viruses

Interaction of Microbe and Host

Epidemiology

Koch's Postulate

XI

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