Laboratory Experiments in Microbiology : An Introduction

by Johnson, Ted R.; Case, Christine L.

• ISBN13: 9780805375893
• ISBN10: 0805375899
• Edition: 6th
• Format: Spiral Bound
• Copyright: 2001-01-01
• Publisher: Benjamin Cummings

Summary

Newly revised to accompany Microbiology: An Introduction, Seventh Edition by Tortora, Funke, and Case, this lab manual includes 57 experiments that demonstrate the broad spectrum of microbiology. Intended as a manual of basic microbiologic techniques, this popular lab manual features applications for undergraduate students in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various preprofessional programs. Experiments have been refined in this new edition to encourage students to develop critical thinking skills as well as learn basic facts and technical skills. Material with direct application to clinical and commercial labs is included whenever possible, and increased emphasis is placed on lab safety.

Table of Contents

Introduction	1	(4)
part one Microscopy	5	(22)
Use and Care of the Microscope	7	(10)
Examination of Living Microorganisms	17	(10)
part two Staining Methods	27	(40)
Preparation of Smears and Simple Staining	29	(6)
Negative Staining	35	(4)
Gram Staining	39	(6)
Acid-Fast Staining	45	(6)
Structural Stains (Endospore, Capsule, and Flagella)	51	(10)
Morphologic Unknown	61	(6)
part three Cultivation of Bacteria	67	(30)
Microbes in the Environment	69	(8)
Transfer of Bacteria: Aseptic Technique	77	(8)
Isolation of Bacteria by Dilution Techniques	85	(8)
Special Media for Isolating Bacteria	93	(4)
part four Microbial Metabolism	97	(42)
Carbohydrate Catabolism	99	(6)
Fermentation of Carbohydrates	105	(8)
Protein Catabolism, Part 1	113	(6)
Protein Catabolism, Part 2	119	(6)
Respiration	125	(4)
Rapid Identification Methods	129	(10)
part five Microbial Growth	139	(24)
Oxygen and the Growth of Bacteria	141	(8)
Determination of a Bacterial Growth Curve: The Role of Temperature	149	(6)
Other Influences on Microbial Growth: Osmotic Pressure and pH	155	(8)
part six Control of Microbial Growth	163	(30)
Physical Methods of Control: Heat	165	(6)
Physical Methods of Control: Ultraviolet Radiation	171	(6)
Chemical Methods of Control: Disinfectants and Antiseptics	177	(4)
Chemical Methods of Control: Antimicrobial Drugs	181	(8)
Effectiveness of Hand Scrubbing	189	(4)
part seven Microbial Genetics	193	(42)
Regulation of Gene Expression	195	(8)
Isolation of Bacterial Mutants	203	(6)
Transformation of Bacteria	209	(4)
DNA Fingerprinting	213	(6)
Genetic Engineering	219	(10)
Ames Test for Detecting Possible Chemical Carcinogens	229	(6)
part eight The Microbial World	235	(32)
Unknown Identification and Bergey's Manual	237	(6)
Fungi: Yeasts	243	(6)
Fungi: Molds	249	(8)
Phototrophs: Algae and Cyanobacteria	257	(6)
Protozoa	263	(4)
part nine Viruses	267	(16)
Isolation and Titration of Bacteriophages	269	(8)
Plant Viruses	277	(6)
part ten Interaction of Microbe and Host	283	(16)
Epidemiology	285	(8)
Koch's Postulates	293	(6)
part eleven Immunology	299	(26)
Nonspecific Resistance	301	(8)
Blood Group Determination: Slide Agglutination	309	(6)
Agglutination Reactions: Microtiter Agglutination	315	(4)
ELISA Technique	319	(6)
part twelve Microorganisms and Disease	325	(40)
Bacteria of the Skin	327	(6)
Bacteria of the Respiratory Tract	333	(8)
Bacteria of the Mouth	341	(6)
Bacteria of the Gastrointestinal Tract	347	(8)
Bacteria of the Urogenital Tract	355	(6)
Identification of an Unknown from a Clinical Sample	361	(4)
part thirteen Microbiology and the Environment	365	(42)
Microbes in Water: Multiple-Tube Technique	367	(8)
Microbes in Water: Membrane Filter Technique	375	(4)
Microbes in Food: Contamination	379	(6)
Microbes Used in the Production of Foods	385	(4)
Microbes in Soil: The Nitrogen and Sulfur Cycles	389	(12)
Microbes in Soil: Bioremediation	401	(6)
Appendices	407	(26)
A Pipetting	409	(2)
B Dilution Techniques and Calculations	411	(4)
C Use of the Spectrophotometer	415	(2)
D Graphing	417	(4)
E Use of the Dissecting Microscope	421	(2)
F Use of the Membrane Filter	423	(2)
G Electrophoresis	425	(2)
H Keys to Bacteria	427	(6)
Index	433