A Laboratory Guide to the Mammalian Embryo

by Gardner, David K.; Lane, Michelle; Watson, Andrew J.

• ISBN13: 9780195142266
• ISBN10: 0195142268
• Format: Paperback
• Copyright: 2004-01-08
• Publisher: Oxford University Press

Summary

This book pulls together the full range of cell culture, biochemical, microscopic, and genetic techniques to study the early mammalian embryo. Until now, there has never been such a comprehensive compendium, though there have been more focused books of protocol, such as Manipulating the MouseEmbryo, from Cold Spring Harbor. This book is intended to appeal to all constituencies, from basic experimental science to clinical and animal science applications.

Table of Contents

Contributors	ix
Abbreviations	xiii
1. Follicle Development in Vitro	3	(21)
Norah Spears, Evelyn E. Telfer, and Alison A. Murray
2. Preparation of Gametes, in Vitro Maturation, in Vitro Fertilization, and Embryo Recovery and Transfer	24	(17)
Michelle Lane and David K. Gardner
3. Culture of the Mammalian Preimplantation Embryo	41	(21)
David K. Gardner and Michelle Lane
4. Assessment of Preimplantation Embryo Development and Viability	62	(14)
Denny Sakkas, Michelle Lane, and David K. Gardner
5. Micromanipulation of Gametes	76	(23)
Gianpiero D. Palermo, Queenie V. Neri, Takumi Takeuchi, Lucinda L. Veeck, and Zev Rosenwaks
6. Micromanipulation: Biopsy	99	(26)
Joyce C. Harper and Alpesh Doshi
7. Analysis of Intracellular Ions in Embryos: pH and Calcium	125	(14)
Michelle Lane, Jay M. Baltz, and David K. Gardner
8. Assessment of Nutrient Uptake, Metabolite Production, and Enzyme Activity	139	(15)
David K. Gardner and Michelle Lane
9. Metabolic Pathway Activity	154	(11)
Don Rieger
10. Confocal Imaging of Structural Molecules in Mammalian Gametes	165	(19)
Calvin Simerly, Ricardo Moreno, João Ramalho-Santos, Laura Hewitson, and Gerald Schatten
11. Fluorescent in situ Hybridisation for Detection of Aneuploidy in Single Human Blastomeres for Preimplantation Genetic Diagnosis	184	(9)
Leeanda Wilton
12. Assessment of Ploidy, Telomere Length, and Telomerase Activity in Oocytes and Embryos	193	(24)
W. Allan King, Dean H. Betts, Wafa Bureau, and Parvathi K. Basrur
13. Microscale RNA Isolation from Mammalian Embryos	217	(8)
Christine Wrenzycki, Andrew J. Watson, and Michele Calder
14. Relative mRNA Transcript Abundance in Early Embryos by Reverse Transcription-Polymerase Chain Reaction	225	(12)
Paul A. De Sousa, and Andrew J. Watson
15. Characterization of Novel Genes during Early Development by Application of Differential Display RT-PCR	237	(10)
David R. Natale and Andrew J. Watson
16. Two-Dimensional Protein Gel Database Analysis of Embryos, Oocytes, and Oocyte-Associated Granulosa Cells	247	(11)
Keith E. Latham, Gula Nourjanova, Karen Wigglesworth, and John J. Eppig
17. Antisense Disruption of Gene Expression in the Preimplantation Embryo	258	(12)
Lisa C. Barcroft, D. Holstead Jones-Taggart, and Andrew J. Watson
18. Gene Subtraction and Analysis	270	(9)
Marc-André Sirard and Claude Robert
19. Analysis of Apoptosis in the Preimplantation Embryo	279	(19)
Daniel R. Brison, Anthony D. Metcalfe, Debra J. Bloor, Helen R. Hunter, Gerard Brady, and Susan J. Kimber
20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo	298	(15)
D. Page Baluch, Christine M. Pauken, and David G. Capco
21. Human Embryonic Stem Cells	313	(21)
Susan E. Lazendorf, Catherine A. Boyd, and Diane L. Wright
22. Spermatogonial Transplantation	334	(18)
Makoto C. Nagano
23. Somatic Cell Nuclear Transfer	352	(23)
Paul De Sousa, William Ritchie, Andras Dinnyes, Tim King, Lesley Paterson, and Ian Wilmut
24. Cryobiology: Slow Freezing Vitrification of Embryos	375	(16)
Tetsunori Mukaida and Magosaburo Kasai
Index	391