Laboratory Manual for Microbiology Fundamentals: A Clinical Approach

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  • Edition: 2nd
  • Format: Lab Manual
  • Copyright: 2015-01-14
  • Publisher: McGraw-Hill Education
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This laboratory manual for allied health or general microbiology has been written with the student in mind. The authors have used their years of teaching microbiology and microbiology laboratory at all levels to identify and relate the fundamental concepts that are important to the understanding of the science and students’ success in their future field. They have included case studies to exemplify the relevance of the science and extensive visual imagery to help students understand and learn the content. Most importantly, the authors hope this manual will help students experience the thrill of bench science and share some of the enthusiasm they have for microbiology, a field of science that is dynamic, exciting and touches every aspect of your life.

Table of Contents

Laboratory Overview: Introduction

Laboratory Safety, Equipment, and Materials

Basic Techniques

Ex 1 Use of the Microscope

Ex 2 Aseptic Techniques

Ex 3 Isolation Streak Plate

Survey of the Microbial World

Ex 4 Microbial Phototrophs: Algae and Cyanobacteria

Ex 5 Protozoans

Ex 6 The Fungi

Ex 7 Motility: Hanging Drop Method

Staining Techniques

Ex 8 Introduction to Staining

Ex 9 Negative Stain

Ex 10 Capsule Stain

Ex 11 Gram Stain

Ex 12 Acid-Fast Staining (Ziehl-Neelsen and Kinyoun Methods)

Ex 13 Endospore Stain (Schaeffer-Fulton Method)

Media: Enriched, Differential, and Selective

Ex 14 Blood Agar

Ex 15 Mannitol Salt Agar

Ex 16 Eosin Methylene Blue Agar

Ex 17 MacConkey Agar

Ex 18 Evaluation of Media


Ex 19 Standard Plate Count

Ex 20 Osmotic Growth

Ex 21 Aerotolerance

Ex 22 Catalase

Ex 23 Oxidase

Control of Microbial Growth

Ex 24 Killing by Ultraviolet Light

Ex 25 Antimicrobial Susceptibility Test (Kirby-Bauer Method)

Ex 26 Hand Washing

Diagnostic Genetics

Ex 27 DNA Fingerprinting

Applied Genetics

Ex 28 Transformation

Microbes & Disease

Ex 29 Parasitic Protozoans

Ex 30 Parasitic Worms

Ex 31 Vectors of Disease

Diagnostic Immunology

Ex 32 Gel Immunoprecipitation (Immunodiffusion)

Ex 33 Passive (Indirect) Agglutination

Identifying Microbes

Ex 34 EnteroPluri-Test

Environmental Microbiology

Ex 35 Soil Microbiology

Hydrolytic Enzymes

Ex 36 Dnase Test

Ex 37 Gelatin Hydrolysis

Ex 38 Starch Hydrolysis

Ex 39 Casien Hydrolysis

Biochemical Testing

Ex 40 Phenol Red Broth

Ex 41 Triple Sugar Iron Agar

Ex 42 Litmus Milk

Ex 43 IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate)

Ex 44 Urease Test

Ex 45 Nitrate Reduction

Putting It All Together

Ex 46 Known Bacteria for Biochemical Testing

Ex 47 Identification of Unknown Bacteria



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