Laboratory Manual and Workbook in Microbiology : Applications to Patient Care

by Mizer, Helen Eckel; Granato, Paul A.; Morello, Josephine A.

• ISBN13: 9780072463545
• ISBN10: 0072463546
• Edition: 7th
• Format: Paperback
• Copyright: 2002-06-13
• Publisher: McGraw-Hill Science/Engineering/Math

Summary

This microbiology laboratory manual is designed especially for the non-majors, health science microbiology courses. The organization reflects the body systems approach and contains specific sections on clinical diagnosis. 36 exercises and 43 experiments cover a broad range of topics.

Table of Contents

1 Basic Techniques of Microbiology

1 Orientation to the Microbiology Laboratory

1 The Microscope

2 Handling and Examining Cultures

2 Microsopic Morphology of Microorganisms

3 Hanging-Drop and Wet-Mount Preparations

4 Simple Stains

3 Differential Stains

5 Gram Stain

6 Acid-Fast Stain

7 Special Stains

4 Cultivation of Mircroorganisms

8 Culture Media

9 Streaking Technique to Obtain Pure Cultures

10 Pour-Plate and Subculture Techniques

11 Culturing Microorganisms from the Environment

2 Destruction of Microorganisms

5 Physical Antimicrobial Agents

12 Moist and Dry Heat

13 The Autoclave

6 Chemical Antimicrobial Agents

14 Disinfectants

15 Antimicrobial Agent Susceptibility Testing and Resistance

3 Diagnostic Microbiology in Action

7 Principles of Diagnostic Microbiology: Culture of Clinical Specimens, Identifying Isolated Microorganisms, Antigen Detection and Nucleic Acid Assays

16 Primary Media for Isolation of Microorganisms

17 Some Metabolic Activities of Bacteria

18 Activities of Bacterial Enzymes

19 Principles of Antigen Detection and Nucleic Acid Assays for Detection and Identification of Microorganisms

8 Microbiology of the Respiratory Tract

20 Staphylococci

21 Streptococci, Pneumococci, and Enterococci

22 Haemophilus, Corynebacteria, and Bordetella

23 Clinical Specimens from the Respiratory Tract

9 Microbiology of the Intestinal Tract

24 The Enterobacteriaceae

25 Clinical Specimens from the Intestinal Tract

10 Microbiology of the Urinary and Genital tracts

26 Urine Culture Techniques

27 Neisseria and Spirochetes

11 Microbial Pathogens Requiring Special Laboratory Techniques; Serological Identification of Patients' Antibodies

28 Anaerobic Bacteria

29 Mycobacteria

30 Mycoplasmas, Rickettsiae, Chlamydiae, Viruses, and Prions

31 Fungi: Yeasts and Molds

32 Protozoa and Animal Parasites

33 Serological Identification of Patients' Antibodies

4 Applied (Sanitary) Microbiology

34 Bacteriologic Analysis of Water

35 Bacteriologic Analysis of Milk