Macromolecular Crystallography Conventional and High Throughput Methods

by Sanderson, Mark; Skelly, Jane V.

• ISBN13: 9780198520979
• ISBN10: 0198520972
• Format: Hardcover
• Copyright: 2007-06-15
• Publisher: Oxford University Press

Summary

Macromolecular Crystallography is the study of macromolecules (proteins and nucleic acids) using X-ray crystallographic techniques in order to determine their molecular structure. The knowledge of accurate molecular structures is a pre-requisite for rational drug design, and forstructure-based function studies to aid the development of effective therapeutic agents and drugs. The successful determination of the complete genome (genetic sequence) of several species (including humans) has recently directed scientific attention towards identifying the structure and function of the complete complement of proteins that make up that species; a new and rapidly growing field ofstudy called 'structural genomics'. There are now several important and well-funded global initiatives in operation to identify all of the proteins of key model species. One of the main requirements for these initiatives is a high-throughput crystallization facility to speed-up the proteinidentification process. The extent to which these technologies have advanced, calls for an updated review of current crystallographic theory and practice. This practical reference book features the latest conventional and high-throughput methods, and includes contributions from a team of internationally recognized leaders and experts. It will be of relevance and use to graduate students, research scientists and professionals currently working in thefield of conventional and high-throughput macromolecular crystallography.

Table of Contents

Skelly Sohi Batuwangala: Classical Cloning, Expression and Purification

Owens, Nettleship, Berrow, Stuart and Stammers High-Throughput Cloning, Expression and Purification

Chayen Automation of Non-Conventional Crystallization Techniques for Screening and Optimisation

Abdel-Meguid Jeruzalmi Sanderson First Analysis of Macromolecular Crystals

Sanderson In-House Macromolecular Data Collection

Abdel-Meguid Solving the Phase Problem Using Isomorphous Replacement

Delarue Molecular Replacement Techniques for High-Throughput Structure Determination

Murthy MAD Phasing

Weeks Furey Application of Direct Methods to Macromolecular Structure Solution

Abrahams Phase Refinement through Density Modification

Morris Perrakis Lamzin Getting a Macromolecular Model: Model Building, Refinement and Validation

Wasserman Smith D'Amico Koss Burley High-Throughput Crystallographic Data Collection at Synchrotons

Carson Electron Density Fitting and Structure Validation

Masquida Francois Werner Westhof RNA Crystallogenesis

Sohi Laponogov Crystallography in the Study of Protein-DNA Interaction

Fry Abrescia Stuart Virus Crystallography

Abdel-Meguid Macromolecular Crystallography in Drug Design

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