Mammalian Cell Viability

by Stoddart, Martin J.

• ISBN13: 9781617791079
• ISBN10: 1617791075
• Format: Hardcover
• Copyright: 2011-04-12
• Publisher: Humana Pr Inc

Summary

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.#xC2;#xA0; In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible.#xC2;#xA0; Written in the highly successful Methods in Molecular Biology#xE2;#x1E;#xA2; series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.#xC2;#xA0;Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Table of Contents

Preface	p. v
Contributors	p. ix
Cell Viability Assays: Introduction	p. 1
Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods	p. 7
Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay	p. 13
WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells	p. 21
Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye	p. 27
The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability	p. 33
Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B and Clonogenic Assays	p. 45
Annexin V/7-AAD Staining in Keratinocytes	p. 57
Measurement of Caspase Activity: From Cell Populations to Individual Cells	p. 65
Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes	p. 81
Multiplexing Cell Viability Assays	p. 91
Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death	p. 103
Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast-Collagen-GAG Constructs	p. 115
Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers	p. 127
Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections	p. 141
Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay	p. 149
Exploiting the Liberation of Zn2+ to Measure Cell Viability	p. 157
Noninvasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs	p. 165
Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test	p. 179
Closed Ampoule Isothermal Microcalorimetry for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses	p. 191
Digital Image Processing of Live/Dead Staining	p. 209
Index	p. 231
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