Editorial Board | p. xiii |
Contributors | p. xv |
Preface | p. xxv |
General Methods | p. 1 |
General Methods: Antibody-Based and Molecular | p. 3 |
Introduction | p. 5 |
Antibody-Based Methods | p. 6 |
Introduction to Molecular Methodology | p. 26 |
Specimen Collection and Handling | p. 49 |
Immunoglobulin Methods | p. 51 |
Introduction | p. 53 |
Immunoglobulin Genes | p. 54 |
Clinical Indications and Applications of Electrophoresis and Immunofixation | p. 66 |
Immunochemical Characterization of Immunoglobulins | p. 71 |
Immunoglobulin Quantification | p. 92 |
Cryoglobulins, Cryofibrinogenemia, and Pyroglobulins | p. 97 |
Complement | p. 107 |
Introduction | p. 109 |
Choosing Complement Tests: Differentiating between Hereditary and Acquired Deficiency | p. 111 |
Molecular Genetic Analyses of Human Complement Components C4A and C4B | p. 117 |
Flow Cytometric Analysis | p. 133 |
Introduction | p. 135 |
Immunophenotyping of Lymphocytes by Flow Cytometry | p. 137 |
Immunophenotyping of Leukemia and Lymphoma by Flow Cytometry | p. 148 |
Functional Assays by Flow Cytometry | p. 160 |
DNA Content Flow Cytometry | p. 171 |
Standardized Flow Cytometry Assays for Enumerating Hematopoietic Stem Progenitor Cells | p. 185 |
Multiplexed Bead Assay in Flow Cytometry: Flow Cytometry-Based Suspension Array Technology | p. 197 |
Cellular Immunology Assays | p. 209 |
Introduction | p. 211 |
Delayed-Type Hypersensitivity Skin Testing | p. 212 |
Cryopreservation of Peripheral Blood Mononuclear Cells | p. 220 |
T-Lymphocyte Activation and Cell Signaling | p. 224 |
Methods for Detection of Antigen-Specific CD8[superscript +] T Cells and Assessment of Their Function | p. 238 |
Evaluation of the T-Cell Receptor Repertoire | p. 244 |
Molecular Diagnosis of Chronic Granulomatous Disease | p. 256 |
Evaluation of Phagocytic Cell Function | p. 265 |
Flow Cytometric Detection and Quantification of Apoptotic Cells | p. 274 |
Non-Flow Cytometry-Based Cell Enumeration Systems | p. 284 |
Quantification of Recent Thymic Emigrants: T-Cell-Receptor Excision Circles | p. 291 |
Measurement of NK-Cell Activity in Humans | p. 296 |
Cellular Immune Assays for Evaluation of Vaccine Efficacy in Developing Countries | p. 301 |
Cytokines, Chemokines, and Adhesion Molecules | p. 318 |
Introduction | p. 319 |
Protein Analysis and Bioassays of Cytokines and Cytokine Receptors | p. 320 |
Flow Cytometric Analysis of Cytokines | p. 338 |
Molecular Analysis of Cytokines and Cytokine Receptors | p. 347 |
Chemokine and Chemokine Receptor Analysis | p. 357 |
Cell Adhesion Molecules: Potential Therapeutic and Diagnostic Implications | p. 368 |
Immunohistology and Immunopathology | p. 378 |
Introduction | p. 379 |
Immunohistochemistry--Principles and Advances | p. 380 |
Immunohistochemistry: Diagnostic and Prognostic Applications in Tumor Diagnosis | p. 387 |
Immunofluorescence Methods in the Diagnosis of Skin and Renal Disease | p. 393 |
Immunologic Diagnosis and Monitoring of Disease | p. 403 |
Infectious Diseases Caused by Bacteria, Mycoplasmas, Chlamydiae, and Rickettsiae | p. 405 |
Introduction | p. 407 |
Immune Response to Streptococcal Antigens: Diagnostic Methods | p. 409 |
Haemophilus influenzae Type b, Neisseria meningitidis, Streptococcus pneumoniae, and Corynebacterium diphtheriae Vaccines | p. 418 |
Clostridium tetani: Immune Response and Diagnostic Methods | p. 432 |
Immunologic Methods for Diagnosis of Infections by Gram-Negative Cocci | p. 435 |
Immunologic Methods for Diagnosis of Infections Caused by Diarrheagenic Members of the Families Enterobacteriaceae and Vibrionaceae | p. 447 |
Serodiagnosis of Helicobacter pylori Infection and Eradication | p. 462 |
Detection of Antibodies to Legionella spp. | p. 468 |
Immunologic Methods for Diagnosis of Spirochetal Diseases | p. 477 |
Lyme Disease: Serodiagnosis of Borrelia burgdorferi Sensu Lato Infection | p. 494 |
Immunological Tests in Tuberculosis and Leprosy | p. 502 |
Nocardiae and Agents of Actinomycosis | p. 511 |
Mycoplasma Infection: Immunologic and Molecular Biologic Diagnostic Techniques | p. 516 |
Chlamydiae | p. 522 |
Immunoserology of the Rickettsiales, Bartonella, and Coxiella | p. 528 |
Mycotic and Parasitic Diseases | p. 543 |
Introduction | p. 545 |
Clinical Immunoparasitology | p. 547 |
Clinical Immunomycology | p. 559 |
Viral Diseases | p. 585 |
Introduction | p. 587 |
Rapid Viral Diagnosis | p. 588 |
Antibody-Enhanced Detection of Viruses in Cell Cultures | p. 598 |
Herpes Simplex Virus | p. 605 |
Varicella-Zoster Virus | p. 611 |
Epstein-Barr Virus | p. 615 |
Cytomegalovirus | p. 627 |
Human Herpesvirus 6, 7, and 8 | p. 635 |
Papovaviruses | p. 646 |
Adenoviruses | p. 652 |
Parvovirus B19 | p. 659 |
Influenza Viruses | p. 666 |
Respiratory Syncytial Virus and the Parainfluenza Viruses | p. 672 |
Measles and Mumps | p. 682 |
Rubella Virus | p. 687 |
Enteroviruses | p. 693 |
Viral Hepatitis | p. 696 |
Enzyme Immunoassays for Detection of Rotavirus Antigen and Antibody | p. 718 |
Arboviruses | p. 727 |
Hantaviruses | p. 736 |
Rabies Virus | p. 742 |
Human T-Cell Lymphotropic Virus Types I and II | p. 749 |
Human Immunodeficiency Virus | p. 755 |
Introduction | p. 757 |
Immunologic Evaluation of Patients with Human Immunodeficiency Virus Infection | p. 758 |
Quantitation of Viremia and Determination of Drug Resistance in Patients with Human Immunodeficiency Virus Infection | p. 772 |
Principles and Procedures of Human Immunodeficiency Virus Serodiagnosis | p. 790 |
Immunodeficiency Diseases | p. 805 |
Introduction | p. 807 |
Genetics of Inherited Immunodeficiency Diseases and Diagnostic Techniques | p. 810 |
Combined B- and T-Cell Deficiencies | p. 819 |
Investigation of Signal Transduction Defects | p. 826 |
Primary Antibody Deficiencies in Children and Adults | p. 830 |
Inherited and Acquired C1-Inhibitor Deficiency | p. 837 |
Genetically Determined Deficiencies of the Complement System: C1, C4, C2, and C3 | p. 842 |
Deficiencies of Mannose-Binding Lectin, the Alternative Pathway, and the Late Complement Components | p. 847 |
Neutropenia and Neutrophil Defects | p. 855 |
Allergic Diseases | p. 865 |
Introduction | p. 867 |
Quantitation and Standardization of Allergens | p. 868 |
In Vivo Diagnostic Allergy Testing Methods | p. 875 |
The Diagnostic Allergy Laboratory | p. 883 |
Tests for Immunological Reactions to Drugs and Occupational Allergens | p. 891 |
Assay Methods for Measurement of Mediators and Markers of Allergic Inflammation | p. 899 |
Immunological Tests for Evaluation of Hypersensitivity Pneumonitis and Allergic Bronchopulmonary Aspergillosis | p. 910 |
Systemic Autoimmune Diseases | p. 919 |
Introduction | p. 921 |
Immunofluorescent Antinuclear Antibody Tests | p. 922 |
Autoantibody Testing by Non-Fluorescein Isothiocyanate (FITC) Methods | p. 933 |
Enzyme-Linked Immunosorbent Assays for Diagnostically Important Antinuclear Antibodies | p. 951 |
Rheumatoid Factors | p. 961 |
Antiphospholipid Antibody Tests | p. 973 |
Antineutrophil Cytoplasmic Antibodies (ANCAs) and ANCA Testing | p. 981 |
Organ-Localized Autoimmune Diseases | p. 987 |
Introduction | p. 989 |
Endocrinopathies | p. 990 |
Autoantibody Profiles of Myasthenia Gravis and Lambert-Eaton Myasthenic Syndrome | p. 1005 |
Autoimmune Autonomic Neuropathy and Neuromuscular Hyperexcitability Disorders | p. 1013 |
Autoantibodies to Glycolipids in Peripheral Neuropathy | p. 1018 |
Detection of Antimitochondrial Autoantibodies in Primary Biliary Cirrhosis and Liver-Kidney Microsomal Antibodies in Autoimmune Hepatitis | p. 1023 |
Skin Diseases | p. 1032 |
Cardiovascular Diseases | p. 1043 |
Kidney and Lung Disease Mediated by Anti-Glomerular Basement Membrane Antibodies: Detection by Western Blot Analysis | p. 1049 |
Autoimmunity of Testis, Ovary, and Spermatozoa | p. 1054 |
Immunologic Testing for Celiac Disease and Inflammatory Bowel Disease | p. 1060 |
Autoimmune Thrombocytopenia | p. 1065 |
Autoimmune Hemolytic Anemia | p. 1070 |
Cancer | p. 1081 |
Introduction | p. 1083 |
Human Tumor Antigens Recognized by T Cells | p. 1085 |
Cancer Vaccines | p. 1091 |
Malignancies of the Lymphoid and Histiocytic Systems | p. 1098 |
Monitoring of Immunologic Therapies | p. 1108 |
The Future of Chip Technologies in Cancer Care and Research | p. 1118 |
Transplantation Immunology and Immunogenetics | p. 1125 |
Immunogenetics: Transplantation and Beyond | p. 1127 |
HLA Typing by Molecular Methods | p. 1134 |
Evaluation of the Humoral Response in Transplantation | p. 1153 |
Evaluation of the Cellular Immune Response in Transplantation | p. 1164 |
Histocompatibility Assessment in Bone Marrow Transplantation | p. 1176 |
Graft Engineering for Hematopoietic Stem Cell Transplantation | p. 1187 |
Minor Histocompatibility Antigens | p. 1201 |
Administration | p. 1209 |
Laboratory Management | p. 1211 |
Introduction | p. 1213 |
Standardization of Immunoassay Methodologies | p. 1215 |
Quality Assurance | p. 1227 |
Planning Statistical Quality Control Procedures | p. 1239 |
Clinical Significance and Cost-Effectiveness of Clinical Immunology Testing | p. 1251 |
Clinical Immunology Laboratory Accreditation, Licensure, and Credentials | p. 1258 |
Establishment and Verification of Reference Values | p. 1271 |
Author Index | p. 1283 |
Subject Index | p. 1285 |
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