Manual of Clinical Laboratory Immunology

by Rose, Noel R.; Conway De Macario, Everly

• ISBN13: 9781555810436
• ISBN10: 1555810438
• Edition: 4th
• Format: Hardcover
• Copyright: 1992-06-01
• Publisher: Amer Society for Microbiology

Table of Contents

Editorial Board	p. xiii
Contributors	p. xv
Preface	p. xxv
General Methods	p. 1
General Methods: Antibody-Based and Molecular	p. 3
Introduction	p. 5
Antibody-Based Methods	p. 6
Introduction to Molecular Methodology	p. 26
Specimen Collection and Handling	p. 49
Immunoglobulin Methods	p. 51
Introduction	p. 53
Immunoglobulin Genes	p. 54
Clinical Indications and Applications of Electrophoresis and Immunofixation	p. 66
Immunochemical Characterization of Immunoglobulins	p. 71
Immunoglobulin Quantification	p. 92
Cryoglobulins, Cryofibrinogenemia, and Pyroglobulins	p. 97
Complement	p. 107
Introduction	p. 109
Choosing Complement Tests: Differentiating between Hereditary and Acquired Deficiency	p. 111
Molecular Genetic Analyses of Human Complement Components C4A and C4B	p. 117
Flow Cytometric Analysis	p. 133
Introduction	p. 135
Immunophenotyping of Lymphocytes by Flow Cytometry	p. 137
Immunophenotyping of Leukemia and Lymphoma by Flow Cytometry	p. 148
Functional Assays by Flow Cytometry	p. 160
DNA Content Flow Cytometry	p. 171
Standardized Flow Cytometry Assays for Enumerating Hematopoietic Stem Progenitor Cells	p. 185
Multiplexed Bead Assay in Flow Cytometry: Flow Cytometry-Based Suspension Array Technology	p. 197
Cellular Immunology Assays	p. 209
Introduction	p. 211
Delayed-Type Hypersensitivity Skin Testing	p. 212
Cryopreservation of Peripheral Blood Mononuclear Cells	p. 220
T-Lymphocyte Activation and Cell Signaling	p. 224
Methods for Detection of Antigen-Specific CD8[superscript +] T Cells and Assessment of Their Function	p. 238
Evaluation of the T-Cell Receptor Repertoire	p. 244
Molecular Diagnosis of Chronic Granulomatous Disease	p. 256
Evaluation of Phagocytic Cell Function	p. 265
Flow Cytometric Detection and Quantification of Apoptotic Cells	p. 274
Non-Flow Cytometry-Based Cell Enumeration Systems	p. 284
Quantification of Recent Thymic Emigrants: T-Cell-Receptor Excision Circles	p. 291
Measurement of NK-Cell Activity in Humans	p. 296
Cellular Immune Assays for Evaluation of Vaccine Efficacy in Developing Countries	p. 301
Cytokines, Chemokines, and Adhesion Molecules	p. 318
Introduction	p. 319
Protein Analysis and Bioassays of Cytokines and Cytokine Receptors	p. 320
Flow Cytometric Analysis of Cytokines	p. 338
Molecular Analysis of Cytokines and Cytokine Receptors	p. 347
Chemokine and Chemokine Receptor Analysis	p. 357
Cell Adhesion Molecules: Potential Therapeutic and Diagnostic Implications	p. 368
Immunohistology and Immunopathology	p. 378
Introduction	p. 379
Immunohistochemistry--Principles and Advances	p. 380
Immunohistochemistry: Diagnostic and Prognostic Applications in Tumor Diagnosis	p. 387
Immunofluorescence Methods in the Diagnosis of Skin and Renal Disease	p. 393
Immunologic Diagnosis and Monitoring of Disease	p. 403
Infectious Diseases Caused by Bacteria, Mycoplasmas, Chlamydiae, and Rickettsiae	p. 405
Introduction	p. 407
Immune Response to Streptococcal Antigens: Diagnostic Methods	p. 409
Haemophilus influenzae Type b, Neisseria meningitidis, Streptococcus pneumoniae, and Corynebacterium diphtheriae Vaccines	p. 418
Clostridium tetani: Immune Response and Diagnostic Methods	p. 432
Immunologic Methods for Diagnosis of Infections by Gram-Negative Cocci	p. 435
Immunologic Methods for Diagnosis of Infections Caused by Diarrheagenic Members of the Families Enterobacteriaceae and Vibrionaceae	p. 447
Serodiagnosis of Helicobacter pylori Infection and Eradication	p. 462
Detection of Antibodies to Legionella spp.	p. 468
Immunologic Methods for Diagnosis of Spirochetal Diseases	p. 477
Lyme Disease: Serodiagnosis of Borrelia burgdorferi Sensu Lato Infection	p. 494
Immunological Tests in Tuberculosis and Leprosy	p. 502
Nocardiae and Agents of Actinomycosis	p. 511
Mycoplasma Infection: Immunologic and Molecular Biologic Diagnostic Techniques	p. 516
Chlamydiae	p. 522
Immunoserology of the Rickettsiales, Bartonella, and Coxiella	p. 528
Mycotic and Parasitic Diseases	p. 543
Introduction	p. 545
Clinical Immunoparasitology	p. 547
Clinical Immunomycology	p. 559
Viral Diseases	p. 585
Introduction	p. 587
Rapid Viral Diagnosis	p. 588
Antibody-Enhanced Detection of Viruses in Cell Cultures	p. 598
Herpes Simplex Virus	p. 605
Varicella-Zoster Virus	p. 611
Epstein-Barr Virus	p. 615
Cytomegalovirus	p. 627
Human Herpesvirus 6, 7, and 8	p. 635
Papovaviruses	p. 646
Adenoviruses	p. 652
Parvovirus B19	p. 659
Influenza Viruses	p. 666
Respiratory Syncytial Virus and the Parainfluenza Viruses	p. 672
Measles and Mumps	p. 682
Rubella Virus	p. 687
Enteroviruses	p. 693
Viral Hepatitis	p. 696
Enzyme Immunoassays for Detection of Rotavirus Antigen and Antibody	p. 718
Arboviruses	p. 727
Hantaviruses	p. 736
Rabies Virus	p. 742
Human T-Cell Lymphotropic Virus Types I and II	p. 749
Human Immunodeficiency Virus	p. 755
Introduction	p. 757
Immunologic Evaluation of Patients with Human Immunodeficiency Virus Infection	p. 758
Quantitation of Viremia and Determination of Drug Resistance in Patients with Human Immunodeficiency Virus Infection	p. 772
Principles and Procedures of Human Immunodeficiency Virus Serodiagnosis	p. 790
Immunodeficiency Diseases	p. 805
Introduction	p. 807
Genetics of Inherited Immunodeficiency Diseases and Diagnostic Techniques	p. 810
Combined B- and T-Cell Deficiencies	p. 819
Investigation of Signal Transduction Defects	p. 826
Primary Antibody Deficiencies in Children and Adults	p. 830
Inherited and Acquired C1-Inhibitor Deficiency	p. 837
Genetically Determined Deficiencies of the Complement System: C1, C4, C2, and C3	p. 842
Deficiencies of Mannose-Binding Lectin, the Alternative Pathway, and the Late Complement Components	p. 847
Neutropenia and Neutrophil Defects	p. 855
Allergic Diseases	p. 865
Introduction	p. 867
Quantitation and Standardization of Allergens	p. 868
In Vivo Diagnostic Allergy Testing Methods	p. 875
The Diagnostic Allergy Laboratory	p. 883
Tests for Immunological Reactions to Drugs and Occupational Allergens	p. 891
Assay Methods for Measurement of Mediators and Markers of Allergic Inflammation	p. 899
Immunological Tests for Evaluation of Hypersensitivity Pneumonitis and Allergic Bronchopulmonary Aspergillosis	p. 910
Systemic Autoimmune Diseases	p. 919
Introduction	p. 921
Immunofluorescent Antinuclear Antibody Tests	p. 922
Autoantibody Testing by Non-Fluorescein Isothiocyanate (FITC) Methods	p. 933
Enzyme-Linked Immunosorbent Assays for Diagnostically Important Antinuclear Antibodies	p. 951
Rheumatoid Factors	p. 961
Antiphospholipid Antibody Tests	p. 973
Antineutrophil Cytoplasmic Antibodies (ANCAs) and ANCA Testing	p. 981
Organ-Localized Autoimmune Diseases	p. 987
Introduction	p. 989
Endocrinopathies	p. 990
Autoantibody Profiles of Myasthenia Gravis and Lambert-Eaton Myasthenic Syndrome	p. 1005
Autoimmune Autonomic Neuropathy and Neuromuscular Hyperexcitability Disorders	p. 1013
Autoantibodies to Glycolipids in Peripheral Neuropathy	p. 1018
Detection of Antimitochondrial Autoantibodies in Primary Biliary Cirrhosis and Liver-Kidney Microsomal Antibodies in Autoimmune Hepatitis	p. 1023
Skin Diseases	p. 1032
Cardiovascular Diseases	p. 1043
Kidney and Lung Disease Mediated by Anti-Glomerular Basement Membrane Antibodies: Detection by Western Blot Analysis	p. 1049
Autoimmunity of Testis, Ovary, and Spermatozoa	p. 1054
Immunologic Testing for Celiac Disease and Inflammatory Bowel Disease	p. 1060
Autoimmune Thrombocytopenia	p. 1065
Autoimmune Hemolytic Anemia	p. 1070
Cancer	p. 1081
Introduction	p. 1083
Human Tumor Antigens Recognized by T Cells	p. 1085
Cancer Vaccines	p. 1091
Malignancies of the Lymphoid and Histiocytic Systems	p. 1098
Monitoring of Immunologic Therapies	p. 1108
The Future of Chip Technologies in Cancer Care and Research	p. 1118
Transplantation Immunology and Immunogenetics	p. 1125
Immunogenetics: Transplantation and Beyond	p. 1127
HLA Typing by Molecular Methods	p. 1134
Evaluation of the Humoral Response in Transplantation	p. 1153
Evaluation of the Cellular Immune Response in Transplantation	p. 1164
Histocompatibility Assessment in Bone Marrow Transplantation	p. 1176
Graft Engineering for Hematopoietic Stem Cell Transplantation	p. 1187
Minor Histocompatibility Antigens	p. 1201
Administration	p. 1209
Laboratory Management	p. 1211
Introduction	p. 1213
Standardization of Immunoassay Methodologies	p. 1215
Quality Assurance	p. 1227
Planning Statistical Quality Control Procedures	p. 1239
Clinical Significance and Cost-Effectiveness of Clinical Immunology Testing	p. 1251
Clinical Immunology Laboratory Accreditation, Licensure, and Credentials	p. 1258
Establishment and Verification of Reference Values	p. 1271
Author Index	p. 1283
Subject Index	p. 1285
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