Marrow Stromal Cell Culture

by Edited by Jon N. Beresford , Maureen E. Owen

• ISBN13: 9780521580212
• ISBN10: 0521580218
• Format: Hardcover
• Copyright: 1998-04-13
• Publisher: Cambridge University Press

Summary

The first book of its kind on marrow stromal cells, and written by leading experts in the field, this concise handbook aims to provide all that is needed to those new to this relatively young area of research. It provides a historical perspective and a summary of present knowledge of the marrow stromal lineages which are currently best understood. Detailed protocols for the isolation, culture and characterization of marrow stromal cell types from human and other animal species are given. The breadth and depth of the subject matter included makes the book equally suitable for new researchers and the more experienced investigator in this rapidly expanding field, including clinicians and bioscientists.

Table of Contents

List of contributors	ix	(2)
Preface to the series	xi	(1)
Preface	xii
1 The marrow stromal cell system	1	(9)
MAUREEN E. OWEN
2 The bone marrow stroma in vivo: ontogeny, structure, cellular composition and changes in disease	10	(16)
PAOLO BIANCO
MARA RIMINUCCI
3 Isolation, purification and in vitro manipulation of human bone marrow stromal precursor cells	26	(17)
STAN GRONTHOS
STEPHEN E. GRAVES
PAUL J. SIMMONS
4 Isolation and culture of human bone-derived cells	43	(24)
ROGER GUNDLE
KARINA STEWART
JOANNE SCREEN
JON N. BERESFORD
5 Marrow stromal adipocytes	67	(21)
JEFFREY M. GIMBLE
6 Osteoblast lineage in experimental animals	88	(23)
JANE E. AUBIN
ALEXA HERBERTSON
7 Chondrocyte culture	111	(17)
RANIERI CANCEDDA
FIORELLA DESCALZI CANCEDDA
BEATRICE DOZIN
8 Osteogenic potential of vascular pericytes	128	(21)
ANA M. SCHOR
ANN E. CANFIELD
Index	149