Mass Spectrometry for Microbial Proteomics

by Editor: Haroun N. Shah (Centre for Infections); Co-Editor: Saheer E. Gharbia

• ISBN13: 9780470665473
• ISBN10: 0470665475
• Format: eBook
• Copyright: 2010-06-01
• Publisher: Wiley-Blackwell

Summary

New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens.

This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject –Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.

Table of Contents

Microbial Characterisation; the Transition from conventional methods to Proteomics

Changing concepts in the characterisation of microbes

Microbial phylogeny and evolution; the change from targeted genes to proteins

Mass Spectrometers and Specific Applications

Range of instruments; criteria for selection for microbial analysis

Tools for rapid Mass spectral analysis

Hybrid Mass Spectrometers

Protein Samples Preparation Techniques

Conventional approaches for sample preparation and 2-D gel electrophoresis applications

Protein fractionation and analysis using multidimensional chromatography (PF2D)

On-Chip sample preparation and concentration using newly developed surfaces prior to high-sensitivity MALDI analysis

News affinity capture methods

Characterisation of microorganisms by Pattern Matching of Mass Spectral Profiles and Biomarker Approaches Requiring Minimal Sample Preparation

Analysis of Surface-Associated Molecules of Intact Bacterial Cells using MALDI- TOF-MS

Analysis based upon Membrane-Bound/Intracellular Protein Patterns using SELDI-TOF-MS

Microbial Disease Biomrakers using ProteinChip Arrays

Rapid identification of microorganisms using stable intracellular proteins Markus Kostrzewa & Wolfgang Pusch, Brucker, Germany *Plus additional chapter on viruses (Catherine Fenselau to be invited)

Targeted Molecules and Analysis of Specific Microorganisms

Analysis of High risk pathogens

Proteome Analysis of E. coli

Comparative DNA sequence analysis and typing using Mass Spectrometry

Analysis based upon whole cell digestion; comparative proteomic of antibiotic resistant and sensitive strains of Staphylococcus aureus

Data Analysis

Artificial Neural Networks of Mass Spectral Data

Devising a platform for microbial identification and comparative analysis

Analysis of 2-D gels using DeCyder EDA (Extended Data Analysis)

Table of Contents provided by Publisher. All Rights Reserved.