The Meningococcal Disease Protocals

by Pollard, Andrew J.; Maiden, Martin C. J.; Moxon, E. Richard

• ISBN13: 9780896038011
• ISBN10: 0896038017
• Format: Hardcover
• Copyright: 2001-07-01
• Publisher: Humana Pr Inc

Summary

Andrew Pollard and Martin Maiden have assembled an impressive collection of the latest molecular and cellular techniques for the development, evaluation, and implementation of vaccines to be used against this dreaded disease. The contributors-leading scientists, clinicians, and public health physicians-describe in detail the many approaches to vaccine design, as well as the assessment of immune response to vaccine candidates and novel vaccine formulations. Timely and comprehensive, Meningococcal Vaccines: Methods and Protocols provides the scientist, public health physician, epidemiologist, clinical microbiologist, and clinician with the essential tools to lay bare the secrets of the meningococcus, and to develop, evaluate, and implement successful new meningococcal vaccines.

Table of Contents

Foreword	v
Preface	vii
Contributors	xv
Meningococcal Vaccines and Vaccine Developments	1	(22)
Ian M. Feavers
Immune Response and Host-Pathogen Interactions	23	(18)
Andrew J. Pollard
David Goldblatt
Purification of Capsular Polysaccharide	41	(8)
Qingling Yang
Harold Jennings
Protein-Polysaccharide Conjugation	49	(6)
Zhongwu Guo
Harold Jennings
N-Propionylation	55	(6)
Zhongwu Guo
Harold Jennings
Outer Membrane Protein Purification	61	(20)
Carmen Arigita
Wim Jiskoot
Matthijs R. Graaf
Gideon F. A. Kersten
Outer Membrane Protein Vesicle Vaccines for Meningococcal Disease	81	(28)
Carl E. Frasch
Loek van Alphen
Johan Holst
Jan T. Poolman
Einar Rosenqvist
Methods for Manipulation of Transferring-Binding Proteins	109	(12)
Leanne M. DeWinter
Anthony B. Schryvers
Methods for the Preparation and Crystallization of Fab Fragments in Complex with Peptide Antigens Derived from N. Meningitidis Outer Membrane Proteins	121	(8)
Richard F. Collins
Jeremy P. Derrick
Application of Optical Biosensor Techniques to the Characterization of PorA-Antibody Binding Kinetics	129	(16)
Janet Suker
Bambos M. Charalambous
Construction of porA Mutants	145	(10)
Peter van der Ley
Loek van Alphen
Construction of LPS Mutants	155	(12)
Peter van der Ley
Liana Steeghs
Recombinant Proteins in Vaccine Development	167	(14)
Myron Christodoulides
Keith A. Jolley
John E. Heckels
Identification of Peptides that Mimic N. meningitidis LOS Epitopes Via the Use of Combinatorial Phage-Display Libraries	181	(18)
Paul J. Brett
Ian M. Feavers
Bambos M. Charalambous
Testing Meningococcal Vaccines for Mitogenicity and Superantigenicity	199	(24)
Alexei A. Delvig
John H. Robinson
Lee Wetzler
Early Experiences with Nasal Vaccines against Meningococcal Disease	223	(18)
Johan Holst
Bjorn Haneberg
Animal Models for Meningococcal Disease	241	(14)
Andrew R. Gorringe
Karen M. Reddin
Pierre Voet
Jan T. Poolman
Determination of Antibody Responses to Meningococcal Antigens by ELISA	255	(20)
Einar Rosenqvist
Helena Kayhty
Andrew J. Pollard
Immunoblot Analysis of Sera from Patients and Vaccinees	275	(14)
Elisabeth Wedege
Serogroup B and C Serum Bactericidal Assays	289	(16)
Ray Borrow
George M. Carlone
A Modified ELISA for Measurement of High-Avidity lgG Antibodies to Meningococcal Serogroup C Polysaccharide that Correlate with Bactericidal Titers	305	(12)
Dan M. Granoff
John J. Donnelly
Whole-Blood Model	317	(14)
Catherine A. Ison
Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry	331	(8)
Terje E. Michaelsen
Audun Aase
T-Cell Responses Against Meningococcal Antigens	339	(10)
Lisbeth Meyer Naess
Fredrik Oftung
Audun Aase
Terje E. Michaelsen
Andrew J. Pollard
CD4 T-Cell Epitope Mapping	349	(12)
Alexei A. Delvig
John H. Robinson
Epitope Mapping	361	(10)
John E. Heckels
Myron Christodoulides
Meningococcal Vaccine Trials	371	(24)
Paddy Farrington
Elizabeth Miller
The Introduction of Group C Conjugate Meningococcal Vaccine into the UK	395	(8)
David M. Salisbury
Index	403