Methods in Avian Embryology

by Bronner-Fraser, Marianne; Matsudaira, Paul

• ISBN13: 9780125641531
• ISBN10: 0125641532
• Format: Hardcover
• Copyright: 1996-04-01
• Publisher: Academic Pr

Summary

A current and authoritative guide, Methods in Avian Embryology presents a combination of classical embryological techniques and modern molecular biological approaches to studying the developing avian embryo. The only one of its kind, this book is specifically devoted to providing a detailed approach to studying avian embryos. It also describes how to use this system to study problems in cell, developmental, and neurobiology. The protocols emphasize microsurgery, histology, and cellular and molecular marking, which are not covered in the usual molecular biology methods manuals. The methods include: embryonic transplantations, cell culture and organ culture, in situ hybridization, classical histological techniques, and retrovirally mediated gene transfer. Key Features * Complete and easy-to-follow procedures * Helpful illustrations * Distinguished group of authors * Wide range of approaches

Table of Contents

Culture and Microsurgical Manipulation of the Early Avian Embryo

Quail-Chick Transplantations

Manipulations of Neural Crest Cells of Their Migratory Pathways

Manipulation of the Avian Segmental Plat in Vivo

Somite Strips: An Embryo Fillet Preparation

Embryo Slices

Operations on Limb Buds of Avian Embryos

Cell Marking and Image Analysis

Gene Transfer Using Replication-Defective Retrovital and Adenoviral Vectors

Manipulating Gene Expression with Replication Competent Retroviruses

Hybridization Analysis of Chick Embryos in Whole Mount and Tissue Sections

Micromass Cultures of Limb and Other Mesenchyme

Organ Cultures and Chorioallantoic Grafts

Autonomic and Sensory Neuron Cultures

Retinal Cultures

Migration and Adhesion Assays

Classical Histology and Cell Birthdating

Time Lapse Cinematography.

Table of Contents provided by Publisher. All Rights Reserved.