Methods in Cell Biology Vol. 42 :... | Buy

Summary

From the Reviews of the First Edition: "This is a good reference manual for multi-user facility faced with a wide variety of biological applications." - CYTOMETRY "Flow Cytometry includes an impressive array of methods applicable to chromosome analysis, plant biology, marine biology, fluorescence, insitu hybridization, and others. It succeeds in providing the reader with good insight into the power of the technology throughout biology." - KENNETH A. AULT, MAINE CYTOMETRY RESEARCH INSTITUTE, MAINE MEDICAL CENTER, IN CANCER CELLS Flow Cytometry, Second Edition provides a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow Cytometry to separate and analyze cells. * * Comprehensive methodological coverage in unique style * In depth treatment of procedures * Description of each procedure's: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the technique * Describe their experience with different instruments and applications to different cell systems * Are the Who's Who in Flow Cytometry * 10 methods cover assessment of apoptosis and other modes of cell death * Practical, handbook-style presentation works in lab or classroom * Printed on acid-free paper * Color plates

A Flow Cytometric Technique for Detection of DNA Repair in Mammalian Cells
Rapid Determination of Cellular Resistance-Related Drug Efflux in Tumor Cells
Glutathione and Cellular Resistance to Anti-Cancer Drugs
Assay of Cell Resistance to Ara-C
Detection of mRNA Species by Flow Cytometry
Primed in Situ Labeling (PRINS) and Fluorescence in Situ Hybridization (FISH)
Molecular Phenotyping by Flow Cytometry
Isolation and Analysis of Somatic Cell Mutants with Defects in Endocytic Traffic
Measurement of Micronuclei by Flow Cytometry
Sperm Chromatin Structure Assay: DNA Denaturability
Fine-Needle Cytopuncture and Flow Cytometry in the Characterization of Human Tumors
Functional NK Assays Using Flow Cytometry
Antibodies to Intermediate Filament Proteins as Probes for Multiparameter Flow Cytometry of Human Cancers
Interactive Data Analysis for Evaluation of B-Cell Neoplasia by Flow Cytometry
Analysis and Sorting of Hematopoietic Stem Cells from Mouse Bone Marrow
Reticulocyte Analysis and Reticulocyte Maturity Index
Analysis of Platelets by Flow Cytometry
Flow Cytometry in Malaria Detection
Large-Scale Chromosome Sorting
Strategies for Rare Cell Detection and Isolation
Cell Sorting of Biohazardous Specimens for Assay of Immune Function
High-Speed Photodamage Cell Sorting: An Evaluation of the ZAPPER Prototype
High-Gradient Magnetic Cell Sorting
Contributions of Flow Cytometry to Studies with Multicell Spheroids
Functional Measurements Using HL-60 Cells
HIV Infection: Diagnosis and Disease Progression Evaluation
Cell-Cycle Analysis of Saccharomyces cerevisiae
Staining and Measurement of DNA in Bacteria
Detection of Specific Microorganisms in Environmental Samples Using Flow Cytometry
Strategies for Flow Cytometric Analysis of Marine Microalgae and Sponge Cells
Flow Cytometry and Sorting of Plant Protoplasts and Cells
Use of Fluorescence-Activated Cell Sorting for Rapid Isolation of Insect Cells Harboring Recombinant Baculovirus
Flow Microsphere Immunoassay for the Quantitative and Simultaneous Detection of Multiple Soluble Analytes
Calibration of Flow Cytometer Detector Systems
Standardization for Flow Cytometry
Phase-Sensitive Detection Methods for Resolving Fluorescence Emission Signals and Directly Quantifying Lifetime
Spectra of Fluorescent Dyes Used in Flow Cytometry
Chapter References
Index
Table of Contents provided by Publisher. All Rights Reserved.

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