Methods in Cell Biology Vol. 48 : Caenorhibditus Elegans: Modern Biological Analysis of an Organism

by Epstein, Henry F.; Shakes, Diane C.

• ISBN13: 9780125641494
• ISBN10: 0125641494
• Format: Hardcover
• Copyright: 1995-10-01
• Publisher: Academic Pr

Summary

The first of its kind, this laboratory handbook emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell,developmental, and molecular biology, as well as in genetics and pharmacology. Four primary sections--Genetic and Culture Methods, Neurobiology, Cell and Molecular Biology, and Genomics and Informatics--reflect the cross-disciplinary nature of C. elegans research. Because C. elegans is a simple and malleable organism with a small genome and few cell types, it provides an elegant demonstration of functions fundamental to multicellular organisms. The discipline has greatly expanded as researchers continue to find this small soil nematode to be the model of choice for studying specific pathways, stages of development, and cell types. By directing its audience not just to tried-and-true recipes for research, but also to databases and other innovative sources of information, this comprehensive collection is intended to guide investigators of C. elegans for years to come. First single-source book detailing explanations of current and classic C. elegans methodologies Diversity and scope of techniques covered expected to be useful to the broadening community of C. elegans researchers for years to come Techniques range from reverse genetics and mutagenesis, to laser ablation and electrophysiology, to in situ hybridization and DNA sequencing methods Appendices include resource information important to the C. elegans community, including the C. elegans Genetics Center and Internet resources like the Worm Community System and ACeDB Illustrated with more than 100 tables and figures

Table of Contents

Genetic and Culture Methods: J.A. Lewis and J.T. Fleming, Basic Culture Methods. P. Anderson, Mutagenesis. R.H.A. Plasterk, Reverse Genetics: From Gene Sequence to Mutant Worm. B.D. Williams, Genetic Mapping with Polymorphic Sequence-Tagged Sites. L.S. Huang and P.W. Sternberg, Genetic Dissection of Developmental Pathways. R.K. Herman, Mosaic Analysis. M. Edgley, D.L. Baillie, D.L. Riddle, and A.M. Rose, Genetic Balancers. Neurobiology: J.B. Rand and C.D. Johnson, Genetic Pharmacology: Interactions between Drugs and Gene Products in C. elegans. T.N. Gannon and C.H. Rankin, Methods of Studying Behavioral Plasticity in C. elegans. C.I. Bargmann and L. Avery, Laser Killing of Cells in C. elegans. L. Avery, D. Raizen, and S. Lockery, Electrophysiological Methods. Cell Biology and Molecular Biology: S.W. L'Hernault and T.M. Roberts, Cell Biology of Nematode Sperm. L.G. Edgar, Blastomere Culture and Analysis. G. Seydoux and A. Fire, Whole-Mount in Situ Hybridization for the Detection of RNA in C. elegans Embryos. D.G. Albertson, R.M. Fishpool, and P.S. Birchall, Fluorescent in Situ Hybridization for the Detection of DNA and RNA. D.M. Miller and D.C. Shakes, Immunofluorescence Microscopy. D.H. Hall, Electron Microscopy and Three-Dimensional Image Reconstruction. H.F. Epstein and F. Liu, Proteins and Protein Assemblies. C. Mello and A. Fire, DNA Transformation. M. Krause, Transcription and Translation. M. Krause, Techniques for Analyzing Transcription and Translation. Genomics and Informatics: A. Coulson, C. Huynh, Y. Kozono, and R. Shownkeen, The Physical Map of the C. elegans Genome. A. Favello, L. Hillier, and R.K. Wilson, Genomic DNA Sequencing Methods. L.L. Fulton, L. Hillier, and R.K. Wilson, Large-Scale Complementary DNA Sequencing Methods. F.H. Eeekman and R. Durbin, ACeDB and Macace. L.M. Shoman, E. Grossman, K. Powell, C. Jamison, and B.R. Schatz, The Worm Community System, Release 2.0 (WCSr2). Appendices: Additional Information and Resources. Caenorhabditis Genetics Center--Laboratory Designations. Gene Names andDescriptions. Chapter References. Subject Index.