Methods in Gene Biotechnology

by Wu, William; Welsh, Michael J.; Kaufman, Peter B.; Zhang, Helen H.

• ISBN13: 9780849326592
• ISBN10: 0849326591
• Format: Paperback
• Copyright: 1997-05-01
• Publisher: CRC Pr I Llc

Summary

Univ. of Michigan, Ann Arbor. Handbook on the current understanding and application of biotechnology methods. Describes basic and advanced techniques with clear explanations of their underlying principles and concepts. Includes trouble-shooting guides. For researchers and students. Plastic-comb spiral binding.

Table of Contents

Approaches to Novel Research

Rapid Isolation of Specific cDNAs or Genes by PCR

Construction and Screening of Subtracted and Complete Expression cDNA Libraries

Subcloning of Genes or DNA Fragments

Nonisotopic and Isotopic DNA or RNA Sequencing

Information Superhighway and Computer Databases of Nucleic Acids and Proteins

Characterization of DNA or Genes by Southern Blot Hybridization

Gene Overexpression by Sense RNA in Mammalian Systems

Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies

Analysis of Gene Expression at the RNA Level

Analysis of Gene Expression at the Protein Level

Analysis of Cellular DNA or Abundance mRNA by Radioactive In Situ Hybridization (RISH)

Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization (FISH)

In Situ PCR Hybridization of Low Copy Genes and In Situ RT-PCR Detection of Low Abundance mRNAs

Isolation and Characterization of Genes from Genomic DNA Libraries

Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression

New Strategies for Gene Knockout

Large Scale Expression and Purification of Recombinant Proteins in Cultured Cells

Index

All chapters contain Troubleshooting Guides and References