Methods in Molecular Genetics Vol. 8 : Human Molecular Genetics

by Adolph, Kenneth W.

• ISBN13: 9780120443109
• ISBN10: 0120443104
• Format: Hardcover
• Copyright: 1996-04-01
• Publisher: Academic Pr

Summary

Human Molecular Genetics is a practical guide to the applications of molecular biology and genetics techniques to human cells. A wide range of experimental procedures for investigating human genes and genomes are presented. * * Mutation Detection in Human Genes - chemical mismatch cleavage, DNA mini-sequencing, SSCP method, RT-PCR, electrophoretic mobility shift assay (EMSA), protein truncation test, chromosome deletion analysis. * Gene Mapping, Cloning, Sequencing - gene linkage determination, large-capacity cloning system, cDNA isolation, differential display method, primer-based DNA sequencing. * Transcription: Promoters, Transcription Factors, mRNA, - promotor mutation analysis, transcription factor identification, mRNA-protein interaction characterization. * RNA Editing, Ribozymes, Antisense RNA-mammalian RNA editing assays, ribozymes as genetic tools, antisense RNA technology. * Genome Recombination, Amplification - recombination assays for mammalian cells, gene amplification measurement. * Receptors, Signal Transduction - intra-cellular receptor characterization, analysis of signal transduction genes. * The Mouse as a Model System for Human Molecular Genetics - mouse genome methods (mouse crosses, somatic cell hybrids, YACs), mouse model for cardiovascular disease.

Table of Contents

Mutation Detection in Human Genes

Gene Mapping, Cloning, Sequencing

Transcription: Promoters, Transcription Factors, Mrna

RNA Editing, Ribozymes, Antisense RNA. Genome Recombination Amplification

Receptors, Signal Transduction

The Mouse as a Model System for Human Molecular Genetics

Table of Contents provided by Publisher. All Rights Reserved.