Methods in Neurosciences Vol. 25 : Receptor Molecular Biology

by Conn, Michael P.; Sealfon, Stuart C.

• ISBN13: 9780121852955
• ISBN10: 0121852954
• Format: Hardcover
• Copyright: 1995-02-01
• Publisher: Academic Pr

Summary

The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Highlights * Cloning * Expression systems * Signal transduction * Structure-function techniques * Antireceptor antibodies * Regulation * 3-D receptor modeling and computational probing

Table of Contents

Receptor Cloning

Approaches to Receptor Cloning

Purification of Receptors

Protein and Peptide Microsequencing: Applications in Neuroscience and Receptor Research

Xenopus Oocytes: A System for Expression Cloning and Structure--Function Studies of Ion Channels and Receptors

Cloning of G Protein-Coupled Opioid Receptors Using Degenerate PCR and Low Stringency Homology Screening

Hybrid Arrest Screening in Oocytes

Receptor Cloning: High-Throughput Sequencing of cDNA Tags for Identification of Novel Genes

Expression and Characterization

Ligand Binding and Second Messenger Assays for Cloned Gq/G11-Coupled Neuropeptide Receptors: The GnRH Receptor

Receptor Expression in Mammalian Cells

Baculovirus Expression of Receptors and Channels

Real-Time Measurements of Receptor Activity: Applications of Microphysiometric Techniques to Receptor Biology

Regulation of Na + -H + Exchange by G Protein-Coupled Receptors

Receptor-Activated Tyrosine Phosphatases: Activity Assays and Molecular Cloning

Studies of Functional Domains of Receptor and Channels

Site-Directed Mutagenesis

Receptor Chimers

Mapping G Protein Coupling Domains by Site-Specific Peptides

Synthesis and Expression of Synthetic Genes: Applications to Structure-Function Studies of Receptors

Use of Receptors Expressed in Escherichia coli to Study Autoimmunity against G Protein-Coupled Membrane Proteins

Integrated Methods for the Construction of Three Dimensional Models and Computational Probing of Structure-Function Relations in G-Protein Coupled Receptors

Localization and Regulation

Anti-fusion Protein Antibodies Specific for Receptor Subtypes

Development of Antireceptor Antibodies Using Synthetic Peptides

Receptor mRNA Measurement by Multiplex Nuclease Protection Assay

Antisense DNA/RNA-Based Strategies to Analysis of Signal Transduction via G Proteins

Index

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