Methods in Neurosciences Vol. 26 : PCR in Neuroscience

by Conn, P. Michael; Sarkar, Gobinda

• ISBN13: 9780121852962
• ISBN10: 0121852962
• Format: Hardcover
• Copyright: 1995-03-01
• Publisher: Academic Pr

Summary

The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Key Features * Direct application of PCR to fresh or frozen clinical specimens (e.g., blood and solid tissue) * Complete retrieval of novel expressed genes by PCR without screening a library * Quantitation by PCR * Mutagenesis by PCR * PCR in AIDS research * Simple and effective protocols for PCR on archival specimens

Table of Contents

Direct PCR on Clinical Specimens

Formamide Low-Temperature PCR: Applications for Direct PCR from Clinical Material

Direct Use of Blood in PCR

Application of Reverse Transcription-Mediated PCR

Use of PCR for Isolation of Neuropeptide Receptor Genes

Lock-Docking Rapid Amplification of cDNA Ends PCR: Strategies and Applications

Single-Cell cDNA-PCR

Gene Synthesis by PCR

PCR-Mediated Synthesis of Chimeric Molecules

PCR-Mediated Gene Synthesis

Quantitative PCR

Quantitative PCR: Analysis of Rare Mitochondrial DNA Mutations in Central Nervous System Tissues

PCR-Aided Transcript Titration Assay: Competitive PCR for Evaluation of Absolute Levels of Rare mRNA Species

Comparative Evaluation of Quantitative PCR Methods

Application of PCR in Mutation Detection

Mutational Detection by Single-Strand Conformational Polymorphism

Detection of Mutation in Yeast hsp60 Gene by PCR

Comparison of the Sensitivity of Single-Strand Conformational Polymorphism and Heteroduplex Methods

Analysis of p53 Mutations in Human Gliomas by RNA Single-Strand Conformational Polymorphism

Generation of Probes by PCR

PCR and Generation of Antisense RNA Probes for Use in RNase Protection Assays

In Vitro Transcription of cRNA from PCR-Generated DNA Fragments

PCR in the Context of Cloning and Constructing Libraries

Direct Cloning of DNA Fragments Generated by PCR

Subtractive cDNA Cloning Using Oligo(dT)30-Latex and PCR

Expression Cloning: PCR versus Episomal Vectors for Rescue of Transfected Genes

Use of PCR for Constructing Jumping Libraries

Site-Directed Mutagenesis by PCR

Site-Directed Mutagenesis by PCR: Substitution, Insertion, Deletion, and Gene Fusion

Use of PCR in Analysis of 5-Flanking Region of Androgen Receptor Gene

Application of PCR in AIDS Research

Detection of HIV-1 in Brain Tissue of Individuals with AIDS by in Situ Gene Amplification

Estimation of Genetic Heterogeneity in Primate T-Cell Lymphoma/Leukemia Viruses by PCR

Use of PCR in Detection of Antisense Transcripts in HTLV-I-Infected Patients and Human T-Cell Lines

Miscellaneous Uses of PCR

Direct Chemiluminescent Sequencing of Double-Stranded PCR Products

Use of PCR to Determine Genomic DNA Target Sites for Zinc Finger Protein Expressed in Mouse Cerebellum

DNA Extraction from Archived Specimens by Sonication

Index

Table of Contents provided by Publisher. All Rights Reserved.