Mhc Protocols

by Powis, Stephen H.; Vaughan, Robert W.

• ISBN13: 9780896035485
• ISBN10: 0896035484
• Format: Hardcover
• Copyright: 2002-10-01
• Publisher: Humana Pr Inc

Summary

A collection by leading experts detail of readily reproducible methods for identifying polymorphisms in the human MHC. Described in step-by-step detail to ensure robust and successful experimental results, these techniques provide DNA-based protocols for the study of polymorphism in HLA class I and II genes and in non-HLA HMC genes associated with human disease (TAP1, TAP 2, C2, C4, and TNF- a). The authors also describe methods for accessing HLA sequence data from electronic databases designed to catalog HLA-region genes and their alleles.

Table of Contents

Preface	v
Contributors	xi
I Databases
HLA Informatics: Accessing HLA Sequences from Sequence Databases	3	(20)
James Robinson
Steven G. E. Marsh
Accessing HLA Sequencing Data Through the 6ace Database	23	(22)
Roger Horton
Stephan Beck
II Polymorphism in Classical and Nonclassical HLA Genes
HLA Typing by Restriction Fragment Length Polymorphism Analysis	45	(16)
Robert W. Vaughan
PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles	61	(6)
Robert W. Vaughan
PCR-Sequence-Specific Oligonucleotide Probe Typing for HLA-A, -B, and -DR	67	(46)
Derek Middleton
F. Williams
HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes	113	(30)
Lori L. Steiner
Priscilla V. Moonsamy
Teodorica L. Bugawan
Ann B. Begovich
PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles	143	(30)
Mike Bunce
HLA Typing With Reference Strand-Mediated Conformation Analysis	173	(18)
J. Rafael Arguello
Martha Perez-Rodriguez
Andrea Pay
Gaby Fisher
Alasdair McWhinnie
J. Alejandro Madrigal
Sequencing Protocols for Detection of HLA Class I Polymorphism	191	(32)
Paul P. J. Dunn
Steven T. Cox
Ann-Margaret Little
HLA-E and HLA-G Typing	223	(14)
Jorge Martinez-Laso
Eduardo Gomez-Casado
Antonio Arnaiz-Villena
Typing Alleles of HLA-DM	237	(12)
Helene Teisserenc
III Polymorphism in Non-HLA MHC Genes
Typing Alleles of TAP1 and TAP2	249	(10)
Stephen H. Powis
Determining Alleles of the C2 Gene by Southern Blotting	259	(10)
Zeng-Bian Zhu
John E. Volanakis
Complement C4 Protein and DNA Typing Methods	269	(28)
Peter M. Schneider
Gottfried Mauff
Typing of Tumor Necrosis Factor Alleles	297	(8)
Anthony Gerard Wilson
Molecular Typing of the MHC Class I Chain-Related Gene Locus	305	(20)
R. W. M. Collins
Henry A. F. Stephens
Robert W. Vaughan
IV Microsatellites
HLA Microsatellite Analysis	325	(8)
Mary Carrington
Index	333