Microbiological Applications : A Laboratory Manual in General Microbiology, Short Version

by Benson, Harold J.

• ISBN13: 9780072318890
• ISBN10: 0072318899
• Edition: 8th
• Format: Paperback
• Copyright: 2001-06-27
• Publisher: McGraw-Hill Science/Engineering/Math

Summary

The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and four-color format make Benson's Microbiological Applications: A Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course, Benson assumes no prior organic chemistry course has been taken.

Table of Contents

Part 1 Microscopy

1 Brightfield Microscopy

2 Darkfield Microscopy

3 Phase-Contrast Microscopy

4 Microscopic Measurements

Part 2 Survey of Microorganisms

5 Protozoa, Algae, and Cyanobacteria

6 Microscopic Invertebrates

7 Aseptic Technique

8 The Bacteria

9 Fungi: Yeasts and Molds

Part 3 Microscope Slide Techniques

10 Negative Staining

11 Smear Preparation

12 Simple Staining

13 Capsular Staining

14 Gram Staining

15 Spore Staining: Two Methods

16 Acid-Fast Staining: Ziehl-Neelsen Method

17 Motility Determination

Part 4 Culture Methods

18 Culture Media Preparation

19 Pure Culture Techniques

20 Cultivation of Anaerobes

21 Bacterial Population Counts

22 Slide Culture: Molds

23 Slide Culture: Autotrophs

24 Bacteriophage: Isolation and Culture

Part 5 Environmental Influences and Control of Microbial Growth

25 Temperature: Effects on Growth

26 Temperature: Lethal Effects

27 pH and Microbial Growth

28 Osmotic Pressure and Bacterial Growth

29 Ultraviolet Light: Lethal Effects

30 Evaluation of Disinfectants: The Use-Dilution Method

31 Evaluation of Antiseptics: The Filter Paper Disk Method

32 Evaluaiton of Alcohol: Its Effectiveness as a Skin Degerming Agent

33 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method

34 Oligodynamic Action

35 Bacterial Mutagenicity and Carcinogenesis: The Ames Test

36 Effectiveness and Hand Scrubbing

Part 6 Identification of Unknown Bacteria

37 Preparation and Care of Stock Cultures

38 Morphological Study of Unknown

39 Cultural Characteristics

40 Physiological Characteristics: Oxidation and Fermentation Tests

41 Physiological Characteristics: Hydrolytic Reactions

42 Physiological Characteristics: Miscellaneous Tests

43 Use of Bergey’s Manual and Identibacter Interactus

Part 7 Miniaturized Multitest Systems

44 Enterobacteriaceae Identification: The API 20E System

45 Enterobacteriaceae Identification: The Enterotube II System

46 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System

47 Staphylococcus Identification: The API Staph-Ident System

Part 8 Microbiology of Water, Food, and Milk

48 Bacteriological Examination of Water Qualtative Tests

49 Bacteriological Examination of Water: The Membrane Filter Method

50 Standard Plate Count of Milk

51 Microbiology of Yogurt Production

52 Microbiology of Alcohol Fermentation

53 Bacterial Counts of Foods

Part 9 Medical Microbiology and Immunology

54 A Synthetic Epidemic

55 The Staphylococci: Isolation and Identification

56 The Streptococci: Isolation and Identification

57 Gram-Negative Intestinal Pathogens

58 Urinary Tract Pathogens

59 Slide Agglutination: Serological Typing

60 Slide Agglutination (Latex) Test: For S. aureus Identification

61 Tube Agglutination Test: The Heterophile Antibody Test

62 Tube Agglutination: The Widal Test

63 Phage Typing

64 White Blood Cell Study: The Differential WBC Count

65 Blood Grouping

66 The Snyder Caries Susceptibility Test

Appendix A Tables

Appendix B Indicators, Stains, Reagents

Appendix C Media

Appendix D Identification Charts

Appendix E The Streptococci

Appendix F Identibacter interactus