Microbiology Lab Manual

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  • Edition: 8th
  • Format: Spiral Bound
  • Copyright: 2010-02-01
  • Publisher: McGraw-Hill Science/Engineering/Math
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Laboratory Exercises in Microbiology, 8/e has been prepared to accompanyPrescott's Microbiology, 8e, written by new authors Joanne Willey, Linda Sherwood, and Christopher Woolverton. Like the text, the laboratory manual provides a balanced introduction to laboratory techniques and principles that are important in each area of microbiology.

Table of Contents

Microscopic Techniques
Bright-Field Light Microscope and Microscopic Measurement of Organisms
The Hanging Drop Slide and Bacterial Motility
Dark-Field Light Microscope
Phase-Contrast Light Microscope
Flourescence Microscope
Bacterial Morphology and Staining
Negative Staining
Smear Preparation and Simple Staining
Gram Stain
Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures
Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)
Capsule Staining
Flagella Staining: West and Difco's SpotTest Methods
Basic Laboratory and Culture Techniques
Sterilization and Microbiological Culture Media Preparation
Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures
Spread-Plate Technique
The Streak-Plate Technique and Different Media
Pour-Plate Technique
Cultivation of Anaerobic Bacteria
Determination of Bacterial Numbers
Biochemical Activities of Bacteria
Carbohydrates I: Fermentation and B-Galactosidase Activity
Carbohydrates II: Triple Sugar Iron Agar Tests
Carbohydrates III: Starch Hydrolysis
Lipids: Lipid Hydrolysis
Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility
Proteins, Amino Acids, and Enzymes II: The IMViC Tests
Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis
Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis
Proteins, Amino Acids, and Enzymes V: Catalase Activity
Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity
Proteins, Amino Acids, and Enzymes VII: Oxidase Test
Proteins, Amino Acids, and Enzymes VIII: Urease Activity
Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Test
Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination
Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction
Rapid Multitest Systems
The APIŽ 20E System
The EnterotubeŽ II System
Unknown Identification
Using the First Edition of Bergey's Manual of Systematic Bacteriology to Identify Bacteria
General Unknown
Environmental Factors Affecting Growth of Microorganisms
Osmotic Pressure
The Effects of Chemical Agents on Bacteria I: Disinfectants
The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method)
Hand Washing, Environmental Sampling, and Microbiological Monitoring
Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods
Environmental and Food Microbiology
Standard Coliform Most Probably Number (MPN)Test and Presence-Absence Coliform Test
Membrane Filter Technique for Coliform and Fecal Streptococci; KONFIRM™ Test for Fecal Coliforms
Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers
Enumeration of Soil Microorganisms
Bacterial Count of a Food Product
Examination of Milk for Bacteria
Medical Microbiology
Hemagglutination Reactions: Blood Groups
Isolation of Normal Microbiota from the Human Body
Aerobic and Anaerobic Endospore-Forming Bacteria
Survey of Selected Eucaryotic Microorganisms
Fungi I: Yeasts
Fungi II: Zygomycota, Ascomyceta, and Basidiomyceta
Microbial Genetics and Genomics
Bacterial Mutation
Bacterial Transformation
Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids
Isolation of Genomic DNA from Baker's Yeast Saccharomyces cerevisiae
Isolation and Purification of Genomic DNA from Escherichia coli
Dilutions with Sample Problems
Metric and English Measurement Equivalents
Transmission-Absorbance Table for Spectrophotometry
pH and pH Indicators
Scientific Notation
Identification Charts
Reagents, Solutions, Stains, and Tests
Culture Media
Sources and Maintenance of Microbiological Stock Cultures
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