MicroRNA and Cancer

by Wu, Wei

• ISBN13: 9781607618621
• ISBN10: 1607618621
• Edition: 1st
• Format: Hardcover
• Copyright: 2010-12-04
• Publisher: Humana Pr Inc

Summary

The tiny microRNAs (miRNAs) can have huge impacts on the regulation of a variety of genes and play crucial roles in the fundamental cellular processes. Recent miRNA studies change the landscape of cancer genetics by scrutinizing the alterations of genome-wide miRNA expressions in most common cancers and their regulatory functions during the development of cancer. The connections between miRNAs and cancer are widespread enough to warrant more comprehensive investigations in the systems biology perspective. In MicroRNA and Cancer: Methods and Protocols, internationally renowned experts provide the latest miRNA knowledge, the various techniques and methodologies currently available for cancer research application. Ranging from the fundamental concepts to practical applications, this book presents:'¢ Overview of microRNA biogenesis, computational prediction of new miRNAs in the cancer genome, and miRNA-based therapeutic approaches for cancer treatment'¢ Detailed experimental protocols in miRNA detection with novel and high-throughput technology, miRNA library cloning, miRNA epigenetic regulation, and miRNA pathway study'¢ Stepwise computational and bioinformatic procedures for miRNA complex networks in cancer genomes with a variety of softwares and programs'¢ Cross-cited notes on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols serves researchers with the basic principles of experimental and computational methods for microRNA study in cancer research and provides a firm grounding for those who wish to further develop their own applications and tailor them to their own specific research needs.

Table of Contents

Preface	p. v
Contributors	p. ix
Reviews
MicroRNA Biogenesis and Cancer	p. 3
Computational Identification of miRNAs Involved in Cancer	p. 23
The Principles of MiRNA-Masking Antisense Oligonucleotides Technology	p. 43
The Concept of Multiple-Target Anti-miRNA Antisense Oligonucleotide Technology	p. 51
Modulation of MicroRNAs for Potential Cancer Therapeutics	p. 59
Protocols
Detection of MicroRNAs in Cultured Cells and Paraffin-Embedded Tissue Specimens by In Situ Hybridization	p. 73
MicroRNA Northern Blotting, Precursor Cloning, and Ago2-Improved RNA Interference	p. 85
miRNA Profiling on High-Throughput OpenArray™ System	p. 101
Silicon Nanowire Biosensor for Ultrasensitive and Label-Free Direct Detection of miRNAs	p. 111
High-Throughput and Reliable Protocols for Animal MicroRNA Library Cloning	p. 123
MicroRNA Regulation of Growth Factor Receptor Signaling in Human Cancer Cells	p. 147
Epigenetic Regulation of MicroRNA Expression in Cancer	p. 165
In Vitro Functional Study of miR-126 in Leukemia	p. 185
Prediction of the Biological Effect of Polymorphisms Within MicroRNA Binding Sites	p. 197
The Guideline of the Design and Validation of MiRNA Mimics	p. 211
Analysis of Targets and Functions Coregulated by MicroRNAs	p. 225
Utilization of SSCprofiler to Predict a New miRNA Gene	p. 243
MicroRNA Profiling Using Fluorescence-Labeled Beads: Data Acquisition and Processing	p. 253
Index	p. 269
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