MicroRNAs: From Basic Science to Disease Biology

by Edited by Krishnarao Appasani , Foreword by Sidney Altman , Victor R. Ambros

• ISBN13: 9780521118552
• ISBN10: 0521118557
• Edition: 1st
• Format: Paperback
• Copyright: 2009-08-20
• Publisher: Cambridge University Press

Summary

MicroRNAs (miRNAs) are RNA molecules, conserved by evolution, that regulate gene expressions and their recent discovery is revolutionising both basic biomedical research and drug discovery. Expression levels of miRNAs have been found to vary between tissues and with developmental stages and hence evaluation of the global expression of miRNAs potentially provides opportunities to identify regulatory points for many different biological processes. This wide-ranging reference work, written by leading experts from both academia and industry, will be an invaluable resource for all those wishing to use miRNA techniques in their own research, from graduate students, post-docs and researchers in academia to those working in R&D in biotechnology and pharmaceutical companies who need to understand this emerging technology. From the discovery of miRNAs and their functions to their detection and role in disease biology, this volume uniquely integrates the basic science with industry application towards drug validation, diagnostic and therapeutic development.

Table of Contents

Foreword

Foreword

Introduction

Discovery of MicroRNAs in Various Organisms:

The microRNAs of C. elegans

Non-coding RNAs - development of man-made vector-based intronic microRNAs

Seeing is believing: strategies for studying microRNA expression

MicroRNAs in limb development

Identification of miRNAs in plant oryza sativa

MicroRNA Functions and RNAi-mediated pathways:

Inhibition of translation initiation by a microRNA

In situ analysis of microRNA expression during vertebrate development

MicroRNA function in the nervous system

Micro RNA expression that controls the amount of branched chain Ãı-ketoacid dehydrogenase in mitochondria of human cells

MicroRNAs and the regulation of leaf shape

Computational Biology of microRNAs:

miRBase: a database of microRNA sequences, targets and nomenclature

Computational prediction of microRNA targets in vertebrates, fruitflies and nematodes

Computational approaches to elucidate miRNA biology

The RNA hybrid approach to microRNA target prediction

Machine learning predicts microRNA target sites

Models of microRNA-target coordination

Detection and Quantitation of MicroRNAs:

Detection and analysis of microRNAs using LNA (locked nucleic acid)-modified probes

Detection and quantitation of microRNAs using the RNA InvaderÃé“ assay

A single molecule method to quantify miRNA gene expression

Real-time quantification of MicroRNAs by TaqManÃé“ assays

Real-time quantification of miRNAs and mRNAs employing universal reverse transcription

MicroRNAs in Disease Biology:

Dysregulation of microRNAs in human malignancy

High throughput microRNAs profiling in cancers

Roles of microRNAs in cancer and development

miR-122 in mammalian liver

MiRNAs in glioblastoma

Role of microRNA pathway in Fragile X mental retardation

Insertion of miRNA125b-1 into immunoglobulin heavy chain gene locus mediated by V(D)J recombination in precursor B cell acute lymphoblastic leukemia

miRNAs in TPA-induced differentiation of HL-60 cells

MiRNAs in skeletal muscle differentiation

Identification and potential function of viral microRNAs

Lost in translation: regulation of HIV-1 by microRNAs and a key enzyme of RNA-directed RNA polymerase

MicroRNAs in Stem Cell Development:

MicroRNAs in the stem cells of the mouse Blastocyst

The role of miRNA in hematopoiesis

MicroRNAs in embryonic stem cell differentiation and prediction of their targets

Generation of single cell microRNA expression profile

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