Misbehaving Proteins

by Murphy, Regina M.; Tsai, Amos M.

• ISBN13: 9780387305080
• ISBN10: 0387305084
• Format: Hardcover
• Copyright: 2006-06-07
• Publisher: Springer Verlag

Summary

This text provides an up-to-date collection of theoretical and experimental studies into protein folding, misfolding, aggregation, and stability. Additionally, issues faced during the development of protein products are illustrated.The first section of contributed papers covers advances in computational efforts to examine protein aggregation. In the middle section, several leading researchers describe fundamental experimental efforts to explore the relationships between protein sequence, folded structure, aggregation kinetics, and aggregate morphology. In the final part of the book, contributors discuss the practical issues arising during product development efforts because of the propensity for engineered proteins to misfold and aggregate.

Table of Contents

Part I. Introduction
Protein Folding, Misfolding, Stability, and Aggregation: An Overview	3	(14)
Regina M. Murphy and Amos M. Tsai
Part II. Mathematical Models and Computational Methods
Nonnative Protein Aggregation: Pathways, Kinetics, and Stability Prediction	17	(30)
Christopher J. Roberts
Simulations of Protein Aggregation: A Review	47	(34)
Carol K. Hall, Hung D. Nguyen, Alexander J. Marchut, and Victoria Wagoner
Part III. Experimental Methods
Elucidating Structure, Stability, and Conformational Distributions during Protein Aggregation with Hydrogen Exchange and Mass Spectrometry	81	(18)
Erik J. Fernandez and Scott A. Tobler
Application of Spectroscopic and Calorimetric Techniques in Protein Formulation Development	99	(26)
Angela Wilcox and Rajesh Krishnamurthy
Small-Angle Neutron Scattering as a Probe for Protein Aggregation at Many Length Scales	125	(22)
Susan Krueger, Derek Ho, and Amos Tsai
Laser Light Scattering as an Indispensable Tool for Probing Protein Aggregation	147	(20)
Regina M. Murphy and Christine C. Lee
X-Ray Diffraction for Characterizing Structure in Protein Aggregates	167	(26)
Hideyo Inouye, Deepak Sharma, and Daniel A. Kirschner
Glass Dynamics and the Preservation of Proteins	193	(24)
Christopher L. Soles, Amos M. Tsai, and Marcus T. Cicerone
Part IV. Fundamental Studies in Model Systems
Folding and Misfolding as a Function of Polypeptide Chain Elongation: Conformational Trends and Implications for Intracellular Events	217	(30)
Silvia Cavagnero and Nese Kurt
Determinants of Protein Folding and Aggregation in P22 Tailspike Protein	247	(18)
Matthew J. Gage, Brian G. Lefebvre, and Anne S. Robinson
Factors Affecting the Fibrillation of α-Synuclein, a Natively Unfolded Protein	265	(22)
Anthony L. Fink
Molten Globule–Lipid Bilayer Interactions and Their Implications for Protein Transport and Aggregation	287	(26)
Lisa A. Kueltzo and C. Russell Middaugh
Part V. Protein Product Development
Self-Association of Therapeutic Proteins: Implications for Product Development	313	(18)
Mary E.M. Cromwell, Chantel Felten, Heather Flores, Jun Liu, and Steven J. Shire
Mutational Approach to Improve Physical Stability of Protein Therapeutics Susceptible to Aggregation: Role of Altered Conformation in Irreversible Precipitation	331	(20)
Margaret Speed Ricci, Monica M. Pallitto, Linda Owens Narhi, Thomas Boone, and David N. Brems
Index	351