Mitosis and Meiosis

by Wilson; Matsudaira; Rieder

• ISBN13: 9780125885409
• ISBN10: 0125885407
• Format: Paperback
• Copyright: 1998-12-16
• Publisher: Elsevier Science

Summary

Mitosis and meiosis are process of nuclear division in cells. This volume is a practical handbook on the modern techniques used to study mitosis and meiosis, with an emphasis on the composition and function of centrosomes, spindle pole bodies, and kinetochore structure. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques. Key Features * Chapters Contain Information On: * How to generate, screen, and study mutants of mitosis in yeast, fungi, and flies * Techniques to best image fluorescent and nonfluorescent tagged dividing cells * The use and action of mitoclastic drugs * How to generate antibodies to mitotic components and inject them into cells * Methods that can also be used to obtain information on cellular processes in nondividing cells

Table of Contents

Contributors

Preface

Isolation of Centrosomes from Drosophila Embryos

Studying the Composition and Function of Centrosomes in Vertebrates

Isolation of Centrosomes from Spisula solidissima Oocytes

Methods for the in Situ Localization of Proteins and DNA in the Centromere-Kinetochore Complex

Three-Dimensional Transmission Electron Microscopy and Its Application to Mitosis Research

Enlightening Mitosis: Construction and Expression of GFP Fusion Proteins

Recombinant p50/Dynamitin as a Tool to Examine the Role of Dynactin in Intracellular Processes

In Vitro Assays for Studying S. cerevisiae Kinetochore Activity

Fluorescent Speckle Microscopy of Spindle Microtubule Assembly and Motility in Living Cells

Polarized Microscopy of Spindles

Micromanipulation of Chromosomes and Spindles in Insect Spermatocytes

Microinjection of Mitotic Cells

Obtaining Antibodies to Spindle Components

Using Antisense Technology to Study Mitosis

The Use and Action of Drugs in Analyzing Mitosis

Correlative Light and Electron Microscopy of Mitotic Cells in Monolayer Cultures

Identification and Characterization of Mitotic Mutations in Drosophila

Methods of Isolating and Analyzing Mitotic Mutants in Aspergillus nidulans

Using GFP Fusion Proteins to Quantitate Microtubule and Spindle Dynamics in Budding Yeast

The Use of Xenopus Egg Extracts to Study Mitotic Spindle Assembly and Function in Vitro

Methods for Studying Cell Division in Higher Plants

Using Sea Urchin Gametes for the Study of Mitosis

Index

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