Modern Size-Exclusion Liquid Chromatography: Practice of Gel Permeation and Gel Filtration Chromatography, 2nd Edition

by Andre Striegel; Wallace W. Yau; Joseph J. Kirkland (E. I. du Pont de Nemours & Co.); Donald D. Bly (E. I. du Pont de Nemours & Company, Wilmington Delaware)

• ISBN13: 9780470442838
• ISBN10: 0470442832
• Format: eBook
• Copyright: 2009-07-01
• Publisher: Wiley

Summary

The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique.Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheologyThroughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.

Table of Contents

Foreword

Preface

Background

Introduction

History

Utility of SEC

Molar Mass Averages and Molar Mass Distribution

Structure of The Book

References

Retention

Introduction

Solute Retention in LC

Solute Retention in SEC

SEC Retention Mechanism

Theoretical Models of SEC Separation

Other Considerations

References

Band Broadening

Introduction

LC Plate Theory

Mechanism of SEC Band Broadening

Influencing Factors

Experimental Methods

References

Resolution

Introduction

Resolution Concept in SEC of Polymers

Molar Mass Accuracy Criterion

Applications of Column Performance Criteria

Pore Geometry and Operational Effects

References

Equipment

Introduction

Extra-Column Effects: General

Mobile-Phase Reservoirs, Inlet Filters, and Degassers

Solvent-Metering Systems (Pumps)

Sample Injectors and Autosamplers

Miscellaneous Hardware

Laboratory Safety

References

The Column

Introduction

Column Packings

Column-Packing Methods

Column Performance

References

Experimental Variables and Techniques

Introduction

Solvent Effects

Substrate Effects

Sample Effects

Laboratory Techniques

Solvent Selection and Preparation

Selection and Use of Standard Reference Materials

Detector Selection

Column Selection and Handling

Chromatographic Design Considerations

Making the Separation

Troubleshooting

References

Calibration

Introduction

Calibration with Narrow-MMD Standards

Calibration with Broad-MMD Standards

Accuracy of Calibration Methods

Actual Molar Mass Across the SEC Elution Curve

Linear Calibration Ranges

Recent Developments and Recommendations on Band-Broadening Correction

References

Physical Detectors

Introduction

Concentration-Sensitive Detectors

Static Light-Scattering Detection

Quasielastic Light-Scattering Detection

Viscometric Detection

SEC3

References

Chemical Detectors

Introduction

Mass Spectrometry

Fourier Transform Infrared Spectroscopy

Nuclear Magnetic Resonance Spectroscopy

Other Chemical Detectors

Coupling of Chemical Detectors

References

Polymer Architecture and Dilute Solution Thermodynamics

Introduction

Long-Chain Branching

Determining the Short-Chain Branching Distribution

Polymer Architecture: Conformation and Topology

Star Polymers

Determining the Persistence Length

Determining the Characteristic Ratio

Local Polydispersity

References

Aqueous SEC

Introduction

Aqueous SEC Columns

Non-Size-Exclusion Effects and Mobile-Phase Additives

Select Applications of Aqueous SEC

References

Oligomeric SEC

Introduction

What is an Oligomer?

Preliminary Considerations

Oligomeric SEC Columns

Select Applications of Oligomeric SEC

Optimizing Resolution in Oligomeric SEC

References

SEC in 2D-LC Separations

Introduction

Principles of 2D Polymer Separations

Designing an Experimental 2D-LC Protocol

Eluent Transfer in 2D-LC

Stop-Flow SEC × LC

Select Applications of 2D-LC

SEC in 3D Separations

References

Special Techniques

Introduction

Preparative SEC

Recycle SEC

High-Speed SEC

Inverse SEC

Vacancy and Differential SEC

Size-Exclusion Electrochromatography

References

High-Temperature SEC and Rheological Connections

Introduction

High-Temperature SEC

Complementarity of SEC and Rheology

References

Symbols

Abbreviations

Index

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