did-you-know? rent-now

Rent More, Save More! Use code: ECRENTAL

did-you-know? rent-now

Rent More, Save More! Use code: ECRENTAL

5% off 1 book, 7% off 2 books, 10% off 3+ books

9780471201724

Modern Size-Exclusion Liquid Chromatography Practice of Gel Permeation and Gel Filtration Chromatography

by ; ; ;
  • ISBN13:

    9780471201724

  • ISBN10:

    0471201723

  • Edition: 2nd
  • Format: Hardcover
  • Copyright: 2009-06-29
  • Publisher: Wiley
  • Purchase Benefits
List Price: $206.87 Save up to $0.03
  • Buy New
    $206.84
    Add to Cart Free Shipping Icon Free Shipping

    PRINT ON DEMAND: 2-4 WEEKS. THIS ITEM CANNOT BE CANCELLED OR RETURNED.

Summary

The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique.Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheologyThroughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.

Author Biography

André M. Striegel, PhD, is Assistant Professor in the Department of Chemistry & Biochemistry at the Florida State University, with previous industrial R&D experience at Solutia. He has published and lectured extensively in the area of polymer chromatography and is the recipient of several national awards in analytical chemistry. WALLACE W. YAU, PhD, received his doctorate in physical chemistry from University of Massachusetts and is currently a scientist specialist at Dow Chemical Co., with past industrial experience at DuPont and Chevron. He has been in GPC research and polymer characterization for the past four decades. JOSEPH J. KIRKLAND, PhD, is author or coauthor of eight widely read books on liquid chromatography, more than 150 peer-reviewed papers, and thirty patents. With over fifty years of industrial experience, he has been the recipient of many major national and international awards in analytical chemistry. DONALD D. BLY, PhD, retired as manager of analytical science, central research, DuPont. He has remained working as a consultant to analytical and analytical-polymer science businesses and has remained active in the American Chemical Society and ASTM.

Table of Contents

Background.
Introduction
History
Utility of SEC
Molar Mass Averages and Molar Mass Distribution
Structure of This Book
Retention.
Introduction
Solute Retention in LC
Solute Retention in SEC
SEC Retention Mechanism
Theoretical Models of SEC Separation
Other Considerations
Band Broadening.
Introduction
LC Plate Theory
Mechanism of SEC Band Broadening
Influencing Factors
Experimental Methods
Resolution.
Introduction
Resolution Concept in SEC of Polymers
Molar Mass Accuracy Criterion
Applications of Column Performance Criteria
Pore Geometry and Operational Effects
EQuipment.
Introduction
Extracolumn Effects - General
Mobile-Phase Reservoirs, Inlet Filters, and Degassers
Solvent-Metering Systems (Pumps
Sample Injectors and Autosamplers
Miscellaneous Hardware
Laboratory Safety
The Column.
Introduction
Column Packings
Column Packing Methods
Column Performance
Experimental Variables And Techniques.
Introduction
Solvent Effects
Substrate Effects
Sample Effects
Laboratory Techniques
Solvent Selection and Preparation
Selection and Use of Standard Reference Materials
Detector Selection
Column Selection and Handling
Chromatographic Design Considerations
Making the Separation
Troubleshooting
Calibration.
Introduction
Calibration with Narrow-MMD Standards
Calibration with Broad-MMD Standards
Accuracy of Calibration Methods
Actual Molar Mass Across the SEC Elution Curve
Linear Calibration Ranges
Recent Developments and Recommendations on Band-Broadening Correction (BBC
Physical Detectors.
Introduction
Concentration-Sensitive Detectors
Static Light Scattering Detection, SLS
Quasi-Elastic Light Scattering Detection, QELS
Viscometric Detection, VISC
SEC3
Chemical Detectors.
Introduction
Mass Spectrometry, MS
Fourier Transform Infrared Spectroscopy, FT-IR
Nuclear Magnetic Resonance Spectroscopy, NMR
Other Chemical Detectors
Coupling of Chemical Detectors
Polymer Architecture And Dilute Solution Thermodynamics.
Introduction
Long-Chain Branching, LCB
Determining the Short-Chain Branching Distribution, SCBD
Polymer Architecture: Conformation and Topology
Star Polymers
Determining the Persistence Length, Lp
Determining the Characteristic Ratio, Cn
Local Polydispersity
Aqueous Sec.
Introduction
Aqueous SEC Columns
Non-Size-Exclusion Effects and Mobile Phase Additives
Select Applications of Aqueous SEC
OLigomeric Sec.
Introduction
What is an Oligomer?
Preliminary Considerations
Oligomeric SEC Columns
Select Applications of Oligomeric SEC
Optimizing Resolution in Oligomeric SEC
Sec In 2D-Lc Separations.
Introduction
Principles of 2D Polymer Separations
Designing an Experimental 2D-LC Protocol
Eluent Transfer in 2D-LC
Stop-Flow SEC - LC
Select Applications of 2D-LC
SEC in 3D Separations
Special Techniques.
Introduction
Preparative SEC
Recycle SEC
High-Speed SEC
Inverse SEC, ISEC
Vacancy and Differential SEC
Size-Exclusion Electrochromatography, SEEC
High-Temperature Sec And Rheological Connections.
Introduction
High-Temperature SEC, HT-SEC
Complementarity of SEC and Rheology
Symbols
Abbreviations
Index
Table of Contents provided by Publisher. All Rights Reserved.

Supplemental Materials

What is included with this book?

The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

The Used, Rental and eBook copies of this book are not guaranteed to include any supplemental materials. Typically, only the book itself is included. This is true even if the title states it includes any access cards, study guides, lab manuals, CDs, etc.

Rewards Program