Molecular Evolution: Producing the Biochemical Data

by Abelson; Simon; Zimmer; White; Cann; Wilson

• ISBN13: 9780121821258
• ISBN10: 0121821250
• Format: Hardcover
• Copyright: 1993-10-12
• Publisher: Elsevier Science

Summary

This book provides a single source of information for all molecular methods. Expert advice is given on sample collection and storage for molecular work.

Table of Contents

Practical Issues in Performing Comparative Molecular Studies

Equipping and Organizing Comparative Laboratory Molecular Genetics

Collection and Storage of Land Plant Samples for Macromolecular Comparisons

Collection and Storage of Vertebrate Samples

Collection and Storage of Invertebrate Samples

Collection and Storage of Fungal and Algal Samples

Comparing Macromolecules: Exploring Biological Diversity: Comparisons at Protein Level

One-Dimensional Electrophoretic Comparisons of Plant Proteins

Starch Gel Electrophoresis in Vertebrates

Two-Dimensional Protein Electrophoresis in Phylogenetic Studies

Ancient Proteins in Fossil Bones

Comparative Immunological Methods

Information Content of Immunological Distances

Comparisons at Nucleic Acid Level

Isolation and Comparison of Nucleic Acids from Land Plants

Nuclear and Organellar Genes

Isolation of DNA from Eukaryotic Algae

Isolation and Characterization of Animal Mitochondrial DNA

Comparative Studies of Genome Content

Evolutionary Analyses of Repetitive DNA Sequences

DNA-DNA Hybridization of Single-Copy DNA Sequences

Comparative Studies of Gene Copy Numbers

Optimal Preparative Methods for Producing Comparative Gene Libraries

Surveying Plant Genomes for Variable Number of Tandem Repeat Loci

Use of Randomly Amplified Polymorphic DNA Markers in Comparative Genome Studies

Molecular Approaches to Mammalian Retrotransposon Isolation

Detection and Characterization of Transposable Elements

DNA-DNA Hybridization Approaches to Species Identification in Small Genome Organisms

Direct Ribosomal-RNA Sequencing for Phylogenetic Studies

In Vivo Analysis of Plant 18S Ribosomal-RNA Structure

Comparison of Nucleic Acids from Microorganisms: Sequencing Approaches

Sequencing of Polymerase Chain Reaction-Amplified DNAs

Rapid Production of Single-Stranded Sequencing Template from Amplified DNA Using Magnetic Beads

DNA Sequences from Old Tissue Remains

Analysis of DNA Sequence Variation at Population Level by Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis

Detection of Variability in Natural Populations of Viruses by Polymerase Chain Reaction

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