Molecular Microbiology

by Persing, David H.; Tenover, Fred C.; Versalovic, James; Tang, Yi-Wei; Unger, Elizabeth R.

• ISBN13: 9781555812218
• ISBN10: 155581221X
• Format: Hardcover
• Copyright: 2003-12-01
• Publisher: Amer Society for Microbiology

Summary

Molecular Microbiology: Diagnostic Principles and Practice takes an innovative approach to the dramatic and exciting advances in diagnostic microbiology. A diverse team of editors presents an authoritative and comprehensive cutting-edge text. An earlier publication, Diagnostic Molecular Microbiology: Principles and Applications, served as the foundation for this new text in a field that continues to expand. Section one covers topics such as reviews of DNA probe technologies and amplification methods, while section two is an in-depth treatment of the use of molecular technologies to detect and characterize bacterial, fungal, and parasitic pathogens. Coverage is given to other vital topics as well. This forward looking text will appeal to microbiologists, pathologists, infectious diseases specialists, and their technologists and trainees.

Table of Contents

Contributors	ix
Preface	xv
DIAGNOSTIC PRINCIPLES
I. Culture Confirmation and Direct-Detection Probes
Application of Fluoresence In Situ Hybridization in Diagnostic Microbiology	3	(16)
Stefan Juretschko
Anne Marie Buccat
Thomas R. Fritsche
DNA Probes for Culture Confirmation and Direct Detection of Bacterial Infections: a Review of Technology	19	(8)
Jun Li
Bruce A. Hanna
Use of Commercial Amplification Tests in the Clinical Microbiology Laboratory: Test Selection and Quality Assurance	27	(12)
Gerri S. Hall
Mark Katanik
Marion Tuohy
Mary Sholtis
II. Nucleic Acid Amplification Methods
Evolution of In Vitro Amplification Methods: the Juice Has Been Worth the Squeeze	39	(4)
David H. Persing
In Vitro Nucleic Acid Amplification Techniques	43	(28)
Randall T. Hayden
Real-Time PCR	71	(14)
Carl T. Wittwer
Noriko Kusukawa
Nucleic Acid Amplification Methods: Laboratory Design and Operations	85	(10)
P. Shawn Mitchell
Jeffrey J. Germer
Robin Patel
Quantitative Molecular Methods: Result Standardization, Interpretation, and Laboratory Quality Control	95	(38)
Donald P. Shepley
Donna M. Wolk
III. Diagnostic Sequencing and Mutation Detection
Mutation Screening Strategies: an Overview	133	(4)
Jeremy A. Pena
James Versalovic
Mutation Scanning	137	(8)
Peter Nollau
Christoph Wagener
Mutation Detection: PCR-Based Approaches	145	(8)
Leen-Jan van Doorn
Automated DNA Sequencing	153	(8)
Timothy M. Alcorn
Steven M. Anderson
Molecular Phylogenetic Analysis	161	(22)
Paul W. Lepp
David A. Relman
IV. Molecular Typing Methods
Bacterial Strain Typing through the Decades: a Personal Reflection	183	(2)
Fred C. Tenover
Pulsed-Field Gel Electrophoresis	185	(12)
Richard V. Goering
PCR Amplification-Based Microbial Typing	197	(26)
Jan L. W. Rademaker
Paul Savelkoul
Genotyping Bacteria by Using Variable-Number Tandem Repeats	223	(12)
Barun Mathema
Barry N. Kreiswirth
Multilocus Sequence Typing: Strain Characterization, Population Biology, and Patterns of Evolutionary Descent	235	(10)
William P. Hanage
Edward J. Feil
Angela B. Brueggemann
Brian G. Spratt
Automated Ribotyping	245	(14)
Michael A. Pfaller
Richard J. Hollis
Molecular Subtyping for Epidemiology: Issues in Comparability of Patterns and Interpretation of Data	259	(10)
Timothy J. Barrett
Efrain Ribot
Bala Swaminathan
V. Detection Methodology
Detection of Amplification Products	269	(4)
Yi-Wei Tang
Gel Electrophoresis, Southern Hybridization, and Restriction Fragment Length Polymorphism Analysis	273	(8)
Raymond P. Podzorski
Microwell Plate Detection Systems for Amplicon Detection and Characterization	281	(4)
Michael J. Loeffelholz
Detection of Pathogenic Organisms with Multicolor Molecular Beacons	285	(10)
Fann Wu
Phyllis Della-Latta
Sanjay Tyagi
Fred Russell Kramer
The Fluorescence Resonance Energy Transfer System	295	(12)
J. R. Uhl
F. R. Cockerill III
Elucidation of Diagnostic and Vaccine Candidates by Mass Spectrometry and Proteomics	307	(16)
John F. Douglass
DIAGNOSTIC APPLICATIONS
VI. Detection and Characterization of Bacterial Pathogens
Detection and Characterization of Bacterial Pathogens by Nucleic Acid Amplification: State-of-the-Art Review	323	(26)
M. Ieven
K. Loens
Herman Goossens
PCR Detection of Haemophilus ducreyi, Treponema pallidum, and Mycoplasma genitalium	349	(12)
Patricia A. Toten
Lisa E. Manhart
Arturo Centurion-Lara
16S Ribosomal DNA Sequence Analysis for Identification of Bacteria in a Clinical Microbiology Laboratory	361	(18)
Chris P. Kolbert
Paul N. Rys
Marlene Hopkins
David T. Lynch
Jeffey J. Germer
Cathal E. O'Sullivan
Andrej Trampuz
Robin Patel
Broad-Range PCR for Detection and Identification of Bacteria	379	(12)
Matthias Maiwald
Detection of Antimicrobial Resistance Genes and Mutations Associated with Antimicrobial Resistance in Microorganisms	391	(16)
Fred C. Tenover
J. Kamile Rasheed
Application of Genomic Information to Diagnosis, Management, and Control of Bacteral Infections: the Staphylococcus aureus Model	407	(12)
Keiichi Hiramatsu
Makoto Kuroda
Tadashi Baba
Teruyo Ito
Keiko Okuma
Detection of Agents Associated with Bioterrorism	419	(10)
James W. Snyder
Donald Jungkind
David H. Persing
VII. Detection and Characterization of Viruses
DNA Virus Detection	429	(12)
Richard H. Scheuermann
Rana Domiati-Saad
Beverly Barton Rogers
RNA Virus Detection	441	(34)
Kristin R. Fiebelkorn
Frederick S. Nolte
Viral Load Teting	475	(14)
Charles E. Hill
Angela M. Caliendo
Viral Genotyping	489	(12)
Angela M. Caliendo
Belinda Yen-Lieberman
Phenotypic Teting of Human Immunodeficiency Virus Type 1 Drug Susceptibility	501	(28)
Christos J. Petropoulos
Emerging Viral Pathogens	529	(14)
Robyn S. Klein
Joseph El Khoury
Molecular Virology: Current and Future Trends	543	(8)
Thomas F. Smith
Mark J. Espy
Mary F. Jones
VIII. Detection and Characterization of Fungal Pathogens
Molecular Detection and Characterization of Fungal Pathogens	551	(10)
Roy F. Chemaly
Gary W. Procop
Sequence-Based Fungal Identification and Classification	561	(8)
Michael R. McGinnis
Gary B.Nunn
Molecular Detection of Antifungal Resistance	569	(10)
Thomas D. Edlind
IX. Detection and Characterization of Parasitic Pathogens
Overview of the Development, Utility, and Future of Molecular Diagnostics for Parasitic Disease	579	(4)
William A. Petri, Jr.
Molecular Approaches for Detection, Species Identification, and Genotyping of Cryptosporidium	583	(20)
Roberta M. O'Connor
Malanie R. Mackay
Honorine D. Ward
Molecular Approaches for Diagnosis of Malaria and Characterization of Genetic Markers of Drug Resistance	603	(16)
Annie-Claude Labbe
Dylan Pillai
Kevin C. Kain
X. Applications of Array Technologies
Hybridization Array Technology	619	(24)
Axel G. Stover
Eric Jeffery
Jiangchun Xu
David H. Persing
XI. Host Genetics and Pharmacogenomics
Beyond Bugs: The Human Genome, Genetic Polymorphism, and Disease Management	643	(12)
Frank Xiangjun Liu
David H. Persing
Yi-Wei Tang
Genetic Polymorphisms of Cytochrome P-450s and Drug Transporters and Infectious Disease Management	655	(16)
Hong-Guang Xie
Richard B. Kim
Host Susceptibility to Microbial Infection and Cancer	671	(8)
Emad M. El-Omar
Charles S. Rabkin
Genomics and DNA Variation: Determinants of Susceptibility and Outcomes in Microbial Diseases	679	(12)
Samuel Broder
XII. Laboratory Standardization, Proficiency-Testing Programs, Quality-Control Standards, and Monitoring
External Quality Assessment and Proficiency Testing in Diagnostic Molecular Microbiology	691	(6)
James Versalovic
Eliabeth R. Unger
Laboratory Controls and Standards	697
Dwight B. DuBois
Justin T. Brown