9781592598403

Molecular Toxicology Protocols

by
  • ISBN13:

    9781592598403

  • ISBN10:

    1592598404

  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2005-01-01
  • Publisher: Humana Press

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

Purchase Benefits

  • Free Shipping Icon Free Shipping On Orders Over $35!
    Your order must be $35 or more to qualify for free economy shipping. Bulk sales, PO's, Marketplace items, eBooks and apparel do not qualify for this offer.
  • eCampus.com Logo Get Rewarded for Ordering Your Textbooks! Enroll Now
List Price: $125.00 Save up to $25.00
  • Rent Book $100.00
    Add to Cart Free Shipping Icon Free Shipping

    TERM
    PRICE
    DUE
    USUALLY SHIPS IN 7-10 BUSINESS DAYS
    *This item is part of an exclusive publisher rental program and requires an additional convenience fee. This fee will be reflected in the shopping cart.

Supplemental Materials

What is included with this book?

Author Biography

Volker M. Arlt: Section of Molecular Carcinogenesis, Institute of Cancer Research, Sutton, Surrey, UK Reetakshi Arora: National Centre of Applied Human Genetics, Jawaharlal Nehru University, Delhi, India Narendra K. Bairwa: National Centre of Applied Human Genetics, Jawaharlal Nehru University, Delhi, India Ramesh Bamezai: National Centre of Applied Human Genetics, Jawaharlal Nehru University, Delhi, India Christopher J. Betti: Program in Molecular Biology, Loyola University Chicago Medical Center, Maywood, IL William F. Blakely: Armed Forces Radiobiology Research Institute, Bethesda, MD Grigory G. Borisenko: Department of Environmental and Occupational Health, University of Pittsburgh, PA Anne-Lise Borresen-Dale: Department of Genetics, The Norwegian Radium Hospital, Oslo, Norway Shama C. Buch: Center for Clinical Pharmacology, University of Pittsburgh, PA R. C. Chaubey: Genetic Toxicology and Chromosome Studies Section, Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, India Walter A. Deutsch: Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA Karen H. Dingley: Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, CA Vasily N. Dobrovolsky: Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR James P. Fabisiak: Department of Environmental and Occupational Health, University of Pittsburgh, PA Elke Feldmann: Institute of Genetics, University of Essen, Essen, Germany James C. Fuscoe: Center for Functional Genomics, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR Weimin Gao: Department of Environmental and Occupational Health, University of Pittsburgh, PA Michael E. Gehring: Department of Pediatrics, Wake Forest University School of Medicine, Winston-Salem, NC Tony E. Godfrey: Department of Medicine, Mount Sinai School of Medicine, New York Wolfgang Goedecke: Institute of Genetics, University of Essen, Germany Stephen G. Grant: Department of Environmental and Occupational Health, University of Pittsburgh, PA Jan Grawe: Cell Analysis Core Facility, Uppsala University, Rudbeck Laboratory, Uppsala, Sweden Karl Otto Greulich: Department for Single Cell and Single Molecule Techniques, Institute for Molecular Biotechnology Jena, Germany Vibhuti Gupta: National Centre of Applied Human Genetics, Jawaharlal Nehru University, Delhi, India Kurt W. Haack: Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory, CA Andreas Hartmann: Novartis Pharma AG, Basel, Switzerland Michael Hausmann: Kirchhoff-Institute for Physics, University of Heidelberg, Germany Tomonori Hayashi: Laboratory of Immunology, Department of Radiobiology/Molecular Epidemiology, Radiation Effects Research Foundation, Hiroshima, Japan John B. Hays: Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis Robert H. Heflich: Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR Vijay Hegde: Pennington Biomedical Research Center, Louisiana State University, Baton Rouge Alan Hewer: Section of Molecular Carcinogenesis, Institute of Cancer Research, Sutton, Surrey, UK Stephen R. Hewitt: Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis Sai-Mei Hou: Department of Biosciences, Karolinska Institute, Huddinge, Sweden T. C. Hsu: Department of Cancer Biology, M. D. Anderson Cancer Center, Houston, TX Maria Jasin: Cell Biology Program, Memorial Sloan-Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, New York Hilde Johnsen: Department of Genetics, The Norwegian Radium Hospital, Oslo, Norway Jennifer M. Johnson: Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, PA Nina Joshi: Department of Environmental and Occupational Health, University of Pittsburgh, PA Valerian E. Kagan: Department of Environmental and Occupational Health, University of Pittsburgh, PA Crystal M. Kelly: Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, PA Lori A. Kelly: Department of Surgery, University of Pittsburgh School of Medicine, PA Phouthone Keohavong: Department of Environmental and Occupational Health, University of Pittsburgh, PA Patrick P. Koty: Department of Pediatrics, Wake Forest University School of Medicine, Winston-Salem, NC Steffi Kuhfittig-Kulle: Institute of Genetics, University of Essen, Germany Yoichiro Kusunoki: Laboratory of Immunology, Department of Radiobiology/Molecular Epidemiology, Radiation Effects Research Foundation, Hiroshima, Japan Seishi Kyoizumi: Laboratory of Immunology, Department of Radiobiology/Molecular Epidemiology, Radiation Effects Research Foundation, Hiroshima, Japan Jean J. Latimer: Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, PA Guro Elisabeth Lind: Department of Genetics, The Norwegian Radium Hospital, Oslo, Norway Ragnhild Lothe: Department of Genetics, The Norwegian Radium Hospital, Oslo, Norway Hitoshi Maeda: Department of Legal Medicine, Osaka City University Medical School, Osaka, Japan Dheeraj K. Malhotra: National Centre of Applied Human Genetics, Jawaharlal Nehru University, Delhi, India Toshinari Minamoto: Divisions of Diagnostic Molecular Oncology and Surgical Oncology, Cancer Research Institute, Kanazawa University, Japan Shirley McCready: School of Biological and Molecular Sciences, Oxford Brookes University, UK Page B. McKinzie: Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR Minako Nagao: Biochemistry Division, National Cancer Center Research Institute, Tokyo, Japan Masako Ochiai: Biochemistry Division, National Cancer Center Research Institute, Tokyo, Japan Andrea Odersky: Institute of Genetics, University of Essen, Germany Iovanna Pandelova: Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis Barbara L. Parsons: Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR Petra Pfeiffer: Institute of Genetics, University of Essen, Germany David H. Phillips: Section of Molecular Carcinogenesis, Institute of Cancer Research, Sutton, Surrey, UK Andrew J. Pierce: Markey Cancer Center, University of Kentucky, Lexington Pataje G. S. Prasanna: Armed Forces Radiobiology Research Institute, Bethesda, MD Anand Ranjan: National Centre of Applied Human Genetics, Jawaharlal Nehru University, Delhi, India Alexander Rapp: Department for Single Cell and Single Molecule Techniques, Institute for Molecular Biotechnology Jena, Germany Marjorie Romkes: Center for Clinical Pharmacology, University of Pittsburgh, PA Anjana Saha: National Centre of Applied Human Genetics, Jawaharlal Nehru University, Delhi, India Joseph G. Shaddock: Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR Kaori Shintani-Ishida: Department of Forensic Medicine, Graduate School of Medicine, University of Tokyo, Japan Therese Sorlie: Department of Genetics, The Norwegian Radium Hospital, Oslo, Norway Gunter Speit: Abteilung Humangenetik, Universitatsklinkum Ulm, Germany Takashi Sugimura: National Cancer Center, Tokyo, Japan Roy R. Swiger: Midwest Research Institute, Palm Bay, FL Vladimir A. Tyurin: Department of Environmental and Occupational Health, University of Pittsburgh, PA Yulia Y. Tyurina: Department of Environmental and Occupational Health, University of Pittsburgh, PA Esther A. Ubick: Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, CA P. T. Udhayasuriyan: National Centre of Applied Human Genetics, Jawaharlal Nehru University, Delhi, India Andrew T. M. Vaughan: Department of Radiation Oncology, Loyola University Chicago Medical Center, Maywood, IL Michael J. Villalobos: Program in Molecular Biology, Loyola University Chicago Medical Center, Maywood, IL John S. Vogel: Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory, CA Phuong Vu: Department of Genetics, The Norwegian Radium Hospital, Oslo, Norway Xifeng Wu: Department of Epidemiology, M. D. Anderson Cancer Center, Houston, TX Liqiang Xi: Hillman Cancer Center, University of Pittsburgh, PA Yun-Ling Zheng: Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, MD Bao-Li Zhu: Department of Legal Medicine, Osaka City University Medical School, Japan

Table of Contents

Prefacep. v
Contributorsp. xi
Analysis of DNA Adducts
[superscript 32]P-Postlabeling Analysis of DNA Adductsp. 3
Modification of the [superscript 32]P-Postlabeling Method to Detect a Single Adduct Species as a Single Spotp. 13
DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometryp. 21
Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpolesp. 29
Analysis of DNA Strand Cleavage at Abasic Sitesp. 39
Detection of Chromosomal and Genome-Wide Damage
Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probesp. 49
Mutagen-Induced Chromatid Breakage as a Marker of Cancer Riskp. 59
Flow Cytometric Analysis of Micronuclei in Erythrocytesp. 69
The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damagep. 85
Computerized Image Analysis Software for the Comet Assayp. 97
The Comet-FISH Technique: A Tool for Detection of Specific DNA Damage and Repairp. 107
DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresisp. 121
Detection and Characterization of Surrogate Gene Mutation
Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytesp. 133
Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systemsp. 145
Methods for Detecting Somatic Mutations In Vitro: The Human T-Cell Cloning Assay Selecting for HPRT Mutantsp. 155
Molecular Analysis of Mutations in the Human HPRT Genep. 161
Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytesp. 171
The GPA In Vivo Somatic Mutation Assayp. 179
Flow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR: Detecting Somatic Mutations in Humans and Micep. 197
Detection and Characterization of Cancer Gene Mutation
Mutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel Electrophoresisp. 207
Analysis of K-RAS and P53 Mutations in Sputum Samplesp. 217
Allele-Specific Competitive Blocker-PCR Detection of Rare Base Substitutionp. 235
Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection: Limitations and Solutionsp. 247
Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesionsp. 263
Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCRp. 279
Analysis of DNA Repair Mechanisms
Microsatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair Machineryp. 293
Unscheduled DNA Synthesis: A Functional Assay for Global Genomic Nucleotide Excision Repairp. 303
Analysis of DNA Repair Using Transfection-Based Host Cell Reactivationp. 321
An Immunoassay for Measuring Repair of Ultraviolet Photoproductsp. 337
Analysis of DNA Double-Strand Break Repair by Nonhomologous End Joining in Cell-Free Extracts From Mammalian Cellsp. 351
Measuring Recombination Proficiency in Mouse Embryonic Stem Cellsp. 373
Array Technologies
Strategies for Measurement of Biotransformation Enzyme Gene Expressionp. 387
Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screeningp. 399
TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Materialp. 415
Development of Quantitative Reverse Transcriptase PCR Assays for Measuring Gene Expressionp. 423
Apoptosis
Quantification of Selective Phosphatidylserine Oxidation During Apoptosisp. 449
Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance Spectroscopy and Annexin-Conjugated Ironp. 457
Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assayp. 465
Indexp. 473
Table of Contents provided by Rittenhouse. All Rights Reserved.

Rewards Program

Reviews for Molecular Toxicology Protocols (9781592598403)