Multiple Myeloma

by Brown, Ross D.; Ho, P. Joy

• ISBN13: 9781588293923
• ISBN10: 1588293920
• Format: Hardcover
• Copyright: 2005-04-30
• Publisher: Humana Pr Inc

Summary

A comprehensive collection of classic and innovative methodologies used in many laboratories for the investigation of multiple myeloma. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine'„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. These proven techniques are ideal for studying the pathogenesis of multiple myeloma and identifying new therapeutic targets.

Table of Contents

Preface	v
Contributors	xi
Multiple Myeloma: Challenges and Opportunities	1	(4)
Douglas Joshua
Ross D. Brown
P. Joy Ho
Immunophenotyping of Plasma Cells in Multiple Myeloma	5	(20)
Gema Mateo Manzanera
Jesus F. San Miguel Izquierdo
Alberto Orfao de Matos
Plasma Cell Labeling Index	25	(12)
Philip R. Greipp
Shaji Kumar
Conventional Cytogenetics in Myeloma	37	(12)
Lynda J. Campbell
Multicolor Spectral Karyotyping in Multiple Myeloma	49	(10)
Jeffrey R. Sawyer
Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridization	59	(12)
Scott VanWier
Rafael Fonseca
Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloma	71	(14)
Pulivarthi H. Rao
Southern Blotting of IgH Rearrangements in B-Cell Disorders	85	(20)
Edna Yuen
Ross D. Brown
Identification and Assembly of V Genes As Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma	105	(16)
Surinder S. Sahota
Mark Townsend
Freda K. Stevenson
Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloma	121	(24)
Brian J. Taylor
Jitra Kriangkum
Erin R. Strachan
Juanita Wizniak
Linda M. Pilarski
Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in Myeloma	145	(20)
Mara Compagno
Barbara Mantoan
Monica Astolfi
Mario Boccadoro
Marco Ladetto
Incomplete DJH Rearrangements	165	(10)
David Gonzalez
Ramon Garcia-Sanz
Identification of Malignant Plasma Cells by mRNA In Situ Hybridization	175	(8)
Ross D. Brown
P. Joy Ho
The SCID-hu Myeloma Model	183	(8)
Joshua Epstein
Shmuel Yaccoby
The 5T2MM Murine Model of Multiple Myeloma: Maintenance and Analysis	191	(16)
Karin Vanderkerken
Kewal Asosingh
Angelo Willems
Hendrik De Raeve
Pedro Couck
Frans Gorus
Peter Croucher
Ben Van Camp
Determination of Telomerase Activity and Telomere Length	207	(18)
Kai-da Wu
Malcolm A. S. Moore
Dendritic Cell/Myeloma Hybrid Vaccine	225	(10)
Dajing Xia
Tim Chan
Jim Xiang
Genetically Engineered Myeloma Cell Vaccine	235	(10)
Siguo Hao
Tim Chan
Jim Xiang
An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors	245	(12)
Andrew C. W. Zannettino
Amanda N. Farrugia
L. Bik To
Gerald J. Atkins
Clonality Detection of the Expanded T-Cell Populations in Patients With Multiple Myeloma	257	(12)
Daniel M-Y. Sze
Detecting Mismatch Repair Defects in Myeloma	269	(10)
Mark Velangi
Elizabeth Matheson
Andrew Hall
Julie Irving
Methylation-Specific Polymerase Chain Reaction	279	(14)
Oliver Galm
James G. Herman
Making Sense of DNA Microarray Data	293	(12)
Wei-min Liu
Sunhee K. Ro
Walter H. Koch
Index	305