Mutation Detection A Practical Approach

by Cotton, R. G. H.; Edkins, E.; Forrest, S.

• ISBN13: 9780199636563
• ISBN10: 0199636567
• Format: Paperback
• Copyright: 1998-05-07
• Publisher: IRL Press

Summary

Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field.

Table of Contents

Single strand conformation polymorphism analysis

Single-stranded conformation polymorphism and heteroduplex analysis

Comprehensive mutation detection with denaturing gradient gel electrophoresis

Cleavage using RNase to detect mutations

Cleavage of mismatched bases using chemical reagents

Mutation detection using T4 endonuclease VII

Detection of mutations by hybridization with sequence-specificoligonucleotide probes

DNA detection and sequence distinction through oligonucleotide ligation

Detection of sequence variation using primer extension

Selective amplification of specific alleles

The protein truncation text (PTT)

Functional assay of the p53 tumour suppressor gene

Advances in direct DNA sequencing for mutation scanning

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