Neural Stem Cells

by Zigova, Tanja; Sanchez-Ramos, Juan Raymond; Sanberg, Paul R.

• ISBN13: 9780896039643
• ISBN10: 0896039641
• Format: Hardcover
• Copyright: 2002-03-01
• Publisher: Humana Pr Inc

Summary

In Neural Stem Cells: Methods and Protocols, internationally recognized experts from academic, clinical, and pharmaceutical laboratories describe in great detail the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These readily reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. Additional techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. The protocols range from the simplest methods of isolation and characterization of neural cell properties to such very sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics.

Table of Contents

Preface	v
Contributors	xi
Part I Isolation and Culture of NSCs
Neural Differentiation of Embryonic Stem Cells	3	(12)
K. Sue O'Shea
Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens	15	(14)
Eric D. Laywell
Valery G. Kukekov
Oleg Suslov
Tong Zheng
Dennis A. Steindler
Isolation of Stem and Precursor Cells from Fetal Tissue	29	(12)
Yuan Y. Wu
Tahmina Mujtaba
Mahendra S. Rao
Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb	41	(8)
Susan C. Barnett
A. Jane Roskams
Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium	49	(6)
Edmund Au
A. Jane Roskams
Production of Immortalized Human Neural Crest Stem Cells	55	(12)
Seung U. Kim
Eiji Nakagawa
Kozo Hatori
Atsushi Nagai
Myung A. Lee
Jung H. Bang
Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization	67	(12)
Lamya S. Shihabuddin
Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood	79	(10)
Shijie Song
J. Sanchez-Ramos
Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers	89	(12)
Erin Lavik
Yang D. Teng
Evan Snyder
Robert Langer
Part II Characterization of NSCs in Vitro
A. Cellular Techniques
Analysis of Cell Generation in the Telencephalic Neuroepithelium	101	(14)
Takao Takahashi
Verne S. Caviness, Jr.
Pradeep G. Bhide
Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures	115	(10)
Angelo L. Vescovi
Rossella Galli
Angela Gritti
Assessing the Involvement of Telomerase in Stem Cell Biology	125	(12)
Mark P. Mattson
Peisu Zhang
Weiming Fu
Detection of Telomerase Activity in Neural Cells	137	(12)
Karen R. Prowse
In Vitro Assays for Neural Stem Cell Differentiation	149	(8)
Marcel M. Daadi
Electron Microscopy and Lac-Z Labeling	157	(22)
Bela Kosaras
Evan Snyder
B. Electrophysiological Techniques
Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells	179	(10)
David S. K. Magnuson
Dante J. Morassutti
C. Molecular Techniques
Fluorescence In Situ Hybridization	189	(8)
Barbara A. Tate
Rachel L. Ostroff
RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells	197	(16)
Theresa E. Gratsch
Differential Display: Isolation of Novel Genes	213	(10)
Theresa E. Gratsch
Cell Labeling and Gene Misexpression by Electroporation	223	(10)
Terence J. Van Raay
Michael R. Stark
Gene Therapy Using Neural Stem Cells	233	(12)
Luciano Conti
Elena Cattaneo
Modeling Brain Pathologies Using Neural Stem Cells	245	(20)
Simonetta Sipione
Elena Cattaneo
Part III Utilization/Characterization of NSCS in Vivo
A. Endogenous Pools of Stem/progenitor Cells
Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model	265	(8)
Marcel M. Daadi
Identification of Musashi1-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods	273	(10)
Yonehiro Kanemura
Shin-ichi Sakakibara
Hideyuki Okano
Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo	283	(8)
Sanjay S. P. Magavi
Jeffrey D. Macklis
Immunocytochemical Analysis of Neuronal Differentiation	291	(8)
Sanjay S. P. Magavi
Jeffrey D. Macklis
Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold	299	(8)
Lisa A. Catapano
Sanjay S. P. Magavi
Jeffrey D. Macklis
B. Transplantation
Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS	307	(12)
Qi-lin Cao
Stephen M. Onifer
Scott R. Whittemore
Optimizing Stem Cell Grafting into the CNS	319	(8)
Scott R. Whittemore
Y. Ping Zhang
Christopher B. Shields
Dante J. Morassutti
David S. K. Magnuson
Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos	327	(14)
Lorenzo Magrassi
Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells	341	(16)
Tanja Zigova
Mary B. Newman
Routes of Stem Cell Administration in the Adult Rodent	357	(18)
Alison E. Willing
Svitlana Garbuzova-Davis
Paul R. Sanberg
Samuel Saporta
Index	375