Neuronal Cell Lines A Practical Approach

by Wood, J. N.

• ISBN13: 9780199633456
• ISBN10: 0199633452
• Format: Paperback
• Copyright: 1992-09-10
• Publisher: IRL Press

Summary

Cell lines have played a significant role in the recent rapid progress that has been made in understanding the molecular events involved in the functioning of the nervous system. Now that systems for generating new cell lines from defined regions of the nervous system have been developed, and the technology for generating transgenic animals with specific gene deletions is available, neuronal cell lines will provide important tools for defining the molecules and mechanisms involved in gene regulation, cell-cell interactions, and the electrical properties of neurons. This instructive book presents clear practical advice on the production and exploitation of neuronal cell lines in areas ranging from transcriptional regulation to the definition of cell adhesion mechanisms. The techniques described will be useful to researchers in neurobiology as well as to physiologists and anatomists interested in molecular biology. The book also provides insight into the technology of cell line generation and the possibilities afforded by transgenic technology.

Table of Contents

Neuronal Hybrid Cell Lines: Generation, Characterization, and Utility

Generation of Neural Cell Lines by Transfer of Viral Oncogenes

Serum-free Media for Neuronal Cell Culture

Embryonal Carcinoma Cells and Embryonic Stem Cells as Models for Neuronal Development and Function

Correlative Electrophysiological and Biochemical Studies in Neuronal Cell Lines

Analysis of Protein Phosphorylation in Cell Lines

Isolation and Characterization of Transfected Cell Lines Expressing Neurotransmitter Receptors Using a Calcium Dye and Flow Cytometry

Regulation of Gate Expression in Neuronal Cell Lines

Cell Lines in Developmental Neurobiology: Assays of Adhesion and Neurite Outgrowth

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