Summary

Developing innovative efficient and sensitive spectroscopic and optical techniques for studying biomedically relevant molecules, structures and processes in vitro and in vivo is a field of rapidly growing interest. This symposium book covers novel and exciting approaches in biomedical spectroscopy. Several chapters deal with infrared and Raman spectroscopy. These complimentary vibrational spectroscopic techniques are capable of monitoring molecular structures as well as structural changes. Such studies are of interest for understanding diseases at a molecular level as well as for developing techniques for efficient early diagnosis based on molecular structural information. The chapters demonstrate also applications vibrational spectroscopy in proteomics and the characterization of micro organisms. The second section of the book introduces surface enhanced Raman scattering (SERS), demonstrates the application of the effect in the biomedical field and develops the concept of multifunctional nanosensors. The measurement of intrinsic optical signals from biological objects such as nerve tissue are discussed in the next section of the book. Chapters deal also with Coherent anti-Stokes Raman scattering (CARS) and fluorescence fluctuation spectroscopy. Other chapters illustrate how photons of very different energies, in the Terahertz and in the ultra violet range, can be used to retrieve molecular structural information from native biomolecules. The electrical properties of protein molecules adsorbed onto a gold substrate are studied by using a scanning Kelvin nanoprobe in a microarray format. The final chapters in the book demonstrate the powerful combination of different spectroscopic techniques for the characterization of biomolecules as well as native and engineered biomaterials. These chapters combine information from Raman and Inelastic Neutron Scattering, optical absorbance and energy dispersive X-ray analysis, positron annihilation lifetime spectroscopy (PALS), 1H NMR, and 129Xe NMR X-ray diffraction and fluorescence resonance energy transfer.

Author Biography

Katrin Kneipp received her Diplom and Ph.D. degree in Physics and Dr.sc. in Physical Chemistry from Friedrich Schiller University in Jena, Germany. Her current research interests at Harvard Medical School include single molecule and nanoscale spectroscopies and their broad interdisciplinary applications.
Ricardo F. Aroca, obtained his undergraduate degree in Chemistry from the University of Chile, PhD in Chemistry from Moscow State University. Presently he is University Professor at the University of Windsor.and fellow of the Chemical Institute of Canada. His research is in surface-enhanced vibrational spectroscopy and its analytical applications. Nanostructure fabrication and characterization.
Harald Kneipp received his Diplom in Physics from Friedrich Schiller University in Jena, and a Ph.D. degree in Physics from the Academy of Sciences in Berlin. He conducted research in the fields of plasma physics, nonlinear optics, and laser physics and development. His current interests include applications of lasers and optical spectroscopy at the frontiers of science and medicine.
After graduating with a BSc (Hons) from University College Dublin (NUI) Edeline Wentrup-Byrne obtained her PhD in organic chemistry from the University of Lausanne in Switzerland. Her research interests include the use of vibrational spectroscopy to study naturally occurring biomaterials, the surface-modification of fluorinated bone-repair materials (ePTFE) and the development of degradable polymeric scaffolds for use in bone repair applications. In addition, she is working with a multi-disciplinary team and a Brisbane-based industry Tissue Therapies to develop a novel skin wound-healing bandage therapy. Currently she is a member of the Tissue Repair and Regeneration Program in the QUT Institute for Health and Biomedical Innovation.

New Tools for Life Science Research Based on Fiber-Optic-Linked Raman and Resonance Raman Spectroscopy	p. 1
Applications of Fourier-Transform Infrared Imaging in Cancer Research	p. 14
Biomedical Aspects of Water Structure in Human and Animal Skin: A Near Infrared-Fourier Transform-Raman Study	p. 30
New Approaches Detection Secondary Conformation of Prion Protein in Frozen-Section Tissue by Fourier-Transform Infrared Microscopy	p. 41
Proteomic Applications of Drop Coating Deposition Raman Spectroscopy	p. 52
Infrared Spectroscopy of Microorganisms: Characterization, Identification, and Differentiation	p. 64
Probing the Influence of the Environment on Microalgae Using Infrared and Raman Spectroscopy	p. 85
Surface-Enhanced Raman Sensing: Glucose and Anthrax	p. 107
Surface-Enhanced Raman Spectroscopy Detection of Hyaluronic Acid: A Potential Biomarker for Osteoarthritis	p. 123
Surface-Enhanced Raman Spectroscopy- and Surface Enhanced Infrared Absorption-Based Molecular Sensors: Selective Detection of Polycyclic Aromatic Hydrocarbons Employing Different Silver NanoparticIe Functionalization Methods	p. 138
Pretein-Nanopartlcle Layer-by-Layer Films as Substrates for Surface-Enhanced Resonance Raman Scattering	p. 152
Surface-Enhanced Raman Scattering of Microorganisms	p. 164
Surface-Enhanced Raman Spectroscopy-Based Optical Labels Deliver Chemical Information from Live Cells	p. 186
Photonic Explorers Based on Multifunctional Nanoplatforms: In Vitro and In Vivo Biomedical Applicatlons	p. 200
Intrinsic Optical Signals in Neural Tissues: Measurements, Mechanisms, and Applications	p. 219
Nonlinear Interferometric Vibrational Imaging: A Method for Distinguishing Coherent Anti-Stokes Raman Scattering from Nonresonant Four-Wave-Mixing Processes and Retrieving Raman Spectra Using Broadband Pulses	p. 236
Single-Molecule Detection at Surfaces: Dual-Color Fluorescence Fluctuation Spectroscopy with Total Internal Reflection Excitation	p. 259
High-Resolution THz Spectroscopy of Crystalline Peptides: Exploring Hydrogen-Bonding Networks	p. 280
Change of Near-UV Circular Dichroism Spectra of Human Hemoglobin upon the R-T Quaternary Structure Transition	p. 297
Kelvin Physics of Protein Layers Printed in Microarray Format	p. 312
Vibrational Spectroscopy Studies on Biologically Relevant Molecules: From Anticancer Agents to Drugs of Abuse	p. 338
Correlative Tools for Measuring External Influences on Protein Folding-Denaturation Using Various Spectroscopic Methods	p. 364
Prediction of Bovine Cartilage Proteoglycan Content Using Energy Dispersive X-ray Analysis or Optical Absorbance and a Multivariat Techniques-Fourier-Transform Infrared Microspectroscopy Model	p. 377
The Effect of Synthetic Conditions on the Free Volume ofPoly(2-hydroxyethyl methacrylate) as studied by [superscript 1]H NMR, [superscript 129]XE NMR, and Positron Annihilation Lifetime Spectroscopy	p. 391
Spectroscopic Investigations into Inactivation of Bacterial Virulence Factors	p. 410
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