New Frontiers in Ultrasensitive Bioanalysis Advanced Analytical Chemistry Applications in Nanobiotechnology, Single Molecule Detection, and Single Cell Analysis

by Xu, Xiao-Hong Nancy

• ISBN13: 9780471746607
• ISBN10: 0471746606
• Edition: 1st
• Format: Hardcover
• Copyright: 2007-04-09
• Publisher: Wiley-Interscience

Summary

An overview of current research and developments in ultrasensitive bioanalysis New platforms of ultrasensitive analysis of biomolecules and single living cells using multiplexing, single nanoparticle sensing, nano-fluidics, and single-molecule detection are advancing every scientific discipline at an unprecedented pace. With chapters written by a diverse group of scientists working in the forefront of ultrasensitive bioanalysis, this book provides an overview of the current status and an in-depth understanding of the objectives and future research directions of ultrasensitive bioanalysis. Spanning a wide spectrum of new research approaches, this book: Introduces new theories, ideas, methodologies, technologies, and applications of ultrasensitive bioanalysis in a wide variety of research fields Includes background, fundamentals, and descriptions of instrumentation and techniques behind every experimental design and approach to help readers explore the promising applications of new tools Covers single molecule detection (SMD), single living cell analysis, multi-functional nanoparticle probes, miniaturization, multiplexing, quantitative and qualitative analysis of metal ions and small molecules, and more Discusses techniques such as single molecule microscope and spectroscopy, single nanoparticle optics, single nanoparticle sensors, micro- and nano-fluidics, microarray detection, ultramicroelectrodes, electrochemiluminescence, mass spectrometry, and more This book will be a useful resource and an inspiration for scientists and graduate and undergraduate students in a wide variety of research fields, including chemistry, biology, biomedical science and engineering, and materials science and engineering.

Author Biography

Xiao-Hong Nancy Xu, PHD, is a tenured Associate Professor of Chemistry and Biochemistry at Old Dominion University in Norfolk, Virginia. Her current research program focuses on the development and application of cutting-edge bionanotechnologies and ultrasensitive analytical and sensing methodologies to address fundamental and practical questions in chemical, biochemical, and biomedical sciences and engineering, and is supported by NSF, NIH, DoE, and DoD. She has served extensively as a peer-reviewer for an array of journals and panels and an invited organizer of symposia in chemistry and bioengineering at national and international conferences.

Table of Contents

Preface	p. vii
Contributors	p. xi
Is One Enough?	p. 1
Dissecting Cellular Activity from Single Genes to Single mRNAs	p. 29
Probing Membrane Transport of Single Live Cells Using Single-Molecule Detection and Single Nanoparticle Assay	p. 41
Nanoparticle Probes for Ultrasensitive Biological Detection and Imaging	p. 71
Tailoring Nanoparticles for the Recognition of Biomacromolecule Surfaces	p. 91
Nanoscale Chemical Analysis of Individual Subcellular Compartments	p. 119
Ultra Sensitive Time-Resolved Near-IR Fluorescence for Multiplexed Bioanalysis	p. 141
Ultrasensitive Microarray Detection of DNA Using Enzymatically Amplified SPR Imaging	p. 169
Ultrasensitive Analysis of Metal Ions and Small Molecules in Living Cells	p. 195
Electrochemistry Inside and Outside Single Nerve Cells	p. 215
Electrochemiluminescence Detection in Bioanalysis	p. 235
Single-Cell Measurements with Mass Spectrometry	p. 269
Outlooks of Ultrasensitive Detection in Bioanalysis	p. 295
Index	p. 301
Table of Contents provided by Ingram. All Rights Reserved.