Summary

NMR Spectroscopy Explained : Simplified Theory, Applications and Examples for Organic Chemistry and Structural Biology provides a fresh, practical guide to NMR for both students and practitioners, in a clearly written and non-mathematical format. It gives the reader an intermediate level theoretical basis for understanding laboratory applications, developing concepts gradually within the context of examples and useful experiments. Introduces students to modern NMR as applied to analysis of organic compounds. Presents material in a clear, conversational style that is appealing to students. Contains comprehensive coverage of how NMR experiments actually work. Combines basic ideas with practical implementation of the spectrometer. Provides an intermediate level theoretical basis for understanding laboratory experiments. Develops concepts gradually within the context of examples and useful experiments. Introduces the product operator formalism after introducing the simpler (but limited) vector model.

Author Biography

Neil E. Jacobsen, PHD, is the NMR Facility Manager in the Department of Chemistry at the University of Arizona in Tucson, where he also teaches graduate-level NMR courses. He received his PhD in organic chemistry at the University of California-Berkeley and gained experience in protein NMR spectroscopy at the University of Washington and at Genentech, Inc.

Preface
Acknowledgments
Fundamentalsof NMR Spectroscopy in Liquids
Introduction to NMR Spectroscopy
Examples: NMR Spectroscopy of Oligosaccharides and Terpenoids
Typical Values of Chemical Shifts and Coupling Constants
Fundamental Concepts of NMR Spectroscopy
Interpretation of Proton (1H) NMR Spectra
Assignment
Effect of Bo Field Strength on the Spectrum
First-Order Splitting Patterns
The Use of 1H-1H Coupling Constants to Determine Stereochemistry and Conformation
Symmetry and Chirality in NMR
The Origin of the Chemical Shift
J Coupling to Other NMR-Active Nuclei
Non-First-Order Splitting Patterns: Strong Coupling
Magnetic Equivalence
NMR Hardware and Software
Sample Preparation
Sample Insertion
The Deuterium Lock Feedback Loop
The Shim System
Tuning and Matching the Probe
NMR Data Acquisition and Acquisition Parameters
Noise and Dynamic Range
Special Topic: Oversampling and Digital Filtering
NMR Data Processing-Overview
The Fourier Transform
Data Manipulation Before the Fourier Transform
Data Manipulation After the Fourier Transform
Carbon-13 ( 13 C) NMR Spectroscopy
Sensitivity of 13 C
Splitting of 13 C Signals
Decoupling
Heteronuclear Decoupling: 1 H Decoupled 13C Spectra
Decoupling Hardware
Decoupling Software: Parameters
The Nuclear Overhauser Effect (NOE)
Heteronuclear Decoupler Modes
NMR Relaxation-Inversion-Recovery and the Nuclear Overhauser Effect (NOE)
The Vector Model
One Spin in a Magnetic Field
A Large Population of Identical Spins: Net Magnetization
Coherence: Net Magnetization in the x-y Plane
Relaxation
Summary of the Vector Model
Molecular Tumbling and NMR Relaxation
Inversion-Recovery: Measurement of T 1 Values
Continuous-Wave Low-Power Irradiation of One Resonance
Homonuclear Decoupling
Presaturation of Solvent Resonance
The Homonuclear Nuclear Overhauser Effect (NOE)
Summary of the Nuclear Overhauser Effect
The Spin Echo and the Attached Proton Test (APT)
The Rotating Frame of Reference
The Radio Frequency (RF) Pulse
The Effect of RF Pulses
Quadrature Detection, Phase Cycling, and the Receiver Phase
Chemical Shift Evolution
Scalar (J) Coupling Evolution
Examples of J-coupling and Chemical Shift Evolution
The Attached Proton Test (APT)
The Spin Echo
The Heteronuclear Spin Echo: Controlling J-Coupling Evolution and Chemical Shift Evolution
Coherence Transfer: INEPT and DEPT
Net Magnetization
Magnetization Transfer
The Product Operator Formalism: Introduction
Single Spin Product Operators: Chemical Shift Evolution
Two-Spin Operators: J-coupling Evolution and Antiphase Coherence
The Effect of RF Pulses on Product Operators
INEPT and the Transfer of Magnetization from 1 H to 13 C
Selective Population Transfer (SPT) as a Way of Understanding INEPT Coherence Transfer
Phase Cycling in INEPT
Intermediate States in Coherence Transfer
Zero- and Double-Quantum Operators
Summary of Two-Spin Operators
Refocused INEPT: Adding Spectral Editing
DEPT: Distortionles
Table of Contents provided by Publisher. All Rights Reserved.

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