Nuclear Transfer Protocols

by Verma, Paul J.; Trounson, Alan O.

• ISBN13: 9781588292803
• ISBN10: 1588292800
• Edition: 1st
• Format: Hardcover
• Copyright: 2006-08-04
• Publisher: Humana Pr Inc

Summary

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included, making this an essential comprehensive handbook for research and commercial laboratories involved in, or intending to work on, nuclear transfer. This book will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.

Table of Contents

Preface	v
Contributors	ix
PART I. NUCLEAR TRANSFER
Origin and Progress of Nuclear Transfer In Nonmammalian Animals	3	(32)
Marie A. Di Berardino
Culture and Maintenance of Nuclear Transfer Embryos
In Vitro Maturation and Embryo Production in Cattle	35	(8)
Marc-Andre Sirard
Karine Coenen
Activation of Oocytes After Nuclear Transfer	43	(16)
Zoltan Machaty
Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos	59	(20)
Bjorn Heindryckx
Josiane Van der Elst
Marc Dhont
Maintenance of Pregnancy in Pigs With Limited Viable Embryos	79	(12)
Tim King
Paul A. De Sousa
Health and Neonatal Care of Bovine Clones	91	(20)
Jacqui T. Forsyth
David N. Wells
Nuclear Transfer Methods
Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice	111	(14)
Young Gie Chung
Shaorong Gao
Keith E. Latham
Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors	125	(10)
Kazuchika Miyoshi
Sezen Arat
Steven L. Stice
A Double Nuclear Transfer Technique for Cloning Pigs	135	(16)
Irina A. Polejaeva
Shawn Walker
Keith Campbell
Nuclear Transfer in Nonhuman Primates	151	(18)
Shoukhrat M. Mitalipov
Don P. Wolf
Cloning of Exotic/Endangered Species: Desert Bighorn Sheep	169	(14)
James ``Buck'' Williams
Taeyoung Shin
Ling Liu
Gabriela Flores-Foxworth
Juan Romano
Alice Blue-McClendon
Duane Kraemer
Mark E. Westhusin
Handmade Somatic Cell Cloning in Cattle	183	(16)
Gabor Vatja
Ian M. Lewis
R. Tayfur Tecirlioglu
Transgenesis
Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells	199	(14)
A. John Clark
Sarah Burl
Chris Denning
Transgenesis Using Nuclear Transfer in Goats	213	(16)
Anthoula Lazaris
Rebecca Keyston
Costas N. Karatzas
Carol L. Keefer
PART II. REPROGRAMMING NUCLEI
Nuclear Reprogramming: An Overview	229	(8)
Takashi Tada
Expression of Imprinted Genes in Cloned Mice	237	(10)
Takashi Kohda
Fumitoshi Ishino
Atsuo Ogura
Nuclear Remodeling Assay in Xenopus Egg Extract	247	(12)
Koichi Gonda
Nobuaki Kikyo
In Vitro Reprogramming of Nuclei and Cells	259	(10)
Anne-Mari Hakelien
Thomas Kuntziger
Kristine G. Gaustad
Anne Marstad
Philippe Collas
Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells	269	(16)
Daniele Pralong
Paul J. Verma
Gene Expression and Methylation Patterns in Cloned Embryos	285	(22)
Christine Wrenzycki
Doris Herrmann
Claudia Gebert
Joseph W. Carnwath
Heiner Niemann
PART III. CONCLUDING REMARKS
Are There Any Normal Clones?	307	(12)
Ian Wilmut
Future and Applications of Cloning	319	(14)
Alan O. Trounson
Index	333