Nucleic Acid Testing for Human Disease

by Lorincz; Attila

• ISBN13: 9781574445435
• ISBN10: 157444543X
• Edition: 1st
• Format: Hardcover
• Copyright: 2006-06-20
• Publisher: CRC Press

Summary

Nucleic Acid Testing for Human Disease describes various techniques including target and signal amplification-based NAT procedures, microarrays, bead-based multiplex assays, in situ hybridization, and SNP techniques. This book discusses RNA expression profiling and laboratory issues such as the need for proper validation of tests intended for routine use. It focuses on specific applications of NATs to human disease diagnosis and treatment, from viral, bacterial, fungal, and protozoan detection to genetic exploration by SNP determinations, patterns of RNA expression, and the growing relevance of epigenetic changes. It also offers different perspectives on future directions in the field.

Table of Contents

Nucleic Acid Diagnostic Technology
Target Amplification-Based Techniques	p. 3
Signal Amplification-Based Techniques	p. 19
Microarrays: Human Disease Detection and Monitoring	p. 65
Bead-Based Flow Systems: From Centralized Laboratories to Genetic Testing in the Field	p. 93
In Situ Hybridization	p. 113
Detection Techniques for Single Nucleotide Polymorphisms	p. 139
RNA Expression Profiling	p. 171
Evolution of the Molecular Microbiology Laboratory	p. 189
Disease Applications of Nucleic Acid Tests
Bacterial Sexually Transmitted Diseases	p. 211
Sexually Transmissible Viral Pathogens: Human Papillomaviruses and Herpes Simplex Viruses	p. 243
Blood-Borne Viruses in Clinical and Diagnostic Virology	p. 275
Molecular Methods for the Diagnosis of Fungal Infections	p. 301
Molecular Diagnostic Approaches in Infectious Disease	p. 331
Cancer Detection and Prognosis	p. 353
Common Inherited Genetic Disorders	p. 373
Future Perspectives on Nucleic Acid Testing	p. 413
Bridging the Gap between Analytical and Clinical Validation	p. 435
Index	p. 457
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