Opioid Research

by Pan, Zhizhong Z.

• ISBN13: 9781588290595
• ISBN10: 158829059X
• Format: Hardcover
• Copyright: 2003-04-01
• Publisher: Humana Pr Inc

Summary

This comprehensive collection of the major cross-disciplinary methods and protocols used in current opioid research covers topics ranging from molecular and genetic techniques, to behavioral analyses of animal models and to clinical practices. The well-practiced authors describe their best molecular techniques for the cloning and expression of opioid receptors, and for the quantitative characterization of their signaling pathways, as well as for mapping the distribution and detecting the expression levels of opioid receptors, opioid peptides, and their messages in both brain tissues and individual cells. Also included are methods for the creation of in vitro and in vivo animal models to study opioid functions, as well as the clinical applications in the treatment of pain and opioid addiction.

Table of Contents

Preface	v
Contributors	ix
PART I. MOLECULAR CHARACTERIZATION OF OPIOID RECEPTORS AND SIGNALING PATHWAYS
1 Molecular Cloning of Opioid Receptors by cDNA Library Screening
Ying-Xian Pan	3	(14)
2 Expression of Opioid Receptors in Mammalian Cell Lines
Ying-Xian Pan	17	(12)
3 Assessing Opioid Regulation of Adenylyl Cyclase Activity in Intact Cells
Deepak R. Thakker, Hatice Z. Ozsoy, and Kelly M. Standifer	29	(10)
4 Analysis of Opioid-Induced Kinase Activation
Lan Ma	39	(8)
5 Study of Opioid Receptor Phosphorylation Using Cell-Labeling Method with 32P-Orthrophosphate
Jia Bei Wang	47	(6)
6 Opioid Receptor Coupling to GIRK Channels: In Vitro Studies Using a Xenopus Oocyte Expression System and In Vivo Studies on Weaver Mutant Mice
Kazutaka Ikeda, Mitsunobu Yoshii, Ichiro Sora, and Toru Kobayashi	53	(12)
7 Identification of Alternatively Spliced Variants from Opioid Receptor Genes
Ying-Xian Pan	65	(14)
PART II. MAPPING AND DETECTION OF ENDOGENOUS OPIOIDS
8 Immunohistochemical Localization of p-, 6- and K-Opioid Receptors Within the Antinociceptive Brainstem Circuits
Alexander E. Kalyuzhny	79	(16)
9 In Situ Hybridization in Neural Tissues
Howard B. Gutstein	95	(12)
10 Quantitative Single-Cell RT-PCR for Opioid Receptors and Housekeeping Genes
Seth C. Silbert	107	(34)
11 Gene Arrays and Proteomics: A Primer
Lionel Mouledous and Howard B. Gutstein	141	(16)
PART III. MODEL SYSTEMS FOR STUDIES ON OPIOID FUNCTIONS
12 Opioid Receptor Oligomerization: Detection and Functional Characterization of Interacting Receptors
Ivone Gomes, Julija Filipovska, and Lakshmi A. Devi	157	(28)
13 Recombinant Opioid Receptors: Structure-Function Relationship
Julija Filipovska, Ivone Gomes, Wei Xu, Chongguang Chen, Lee- Yuan Liu-Chen, and Lakshmi A. Devi	185	(20)
14 Receptor Knock-Out and Gene Targeting: Generation of Knock-Out Mice
Ichiro Sora, Kazutaka Ikeda, and Yuji Mishina	205	(12)
15 Pharmacological Interventions at the Spinal Cord: Intrathecal Injections
Daphne A. Robinson and Min Zhuo	217	(6)
16 Opioid Tolerance in Adult and Neonatal Rats
Zhizhong Z. Pan	223	(10)
17 Animal Models of Neuropathic Pain
William J. Martin, Laike St. A. Stewart, and Jason W. Tarpley	233	(10)
18 Place Conditioning to Study Drug Reward and Aversion
William A. Carlezon, Jr	243	(8)
19 Opiate Self-Administration
Zheng-Xiong Xi and Elliot A. Stein	251	(16)
PART IV. CLINICAL APPLICATIONS OF OPIOIDS
20 Acute, Chronic, and Cancer Pain: Clinical Management
Allen W. Burton	267	(18)
21 Clinical Treatment of Opioid Addiction and Dependence
Walter Ling, Richard A. Rawson and Margaret Compton	285	(12)
Index	297