Paramagnetic Resonance of Metallobiomolecules

by Telser, Joshua

• ISBN13: 9780841238329
• ISBN10: 0841238324
• Format: Hardcover
• Copyright: 2003-09-18
• Publisher: American Chemical Society

Summary

Paramagnetic transition metal ions provide the opportunity for application of a wide variety of spectroscopic techniques. Although spectroscopic techniques are not as well known as conventional nuclear magnetic resonance (NMR), they provide detailed information centered on the active site itself. This book describes the application of paramagnetic resonance techniques to a number of important systems in metallobiochemistry. Simultaneously, this volume provides information on novel experimantal methods and theoretical approaches to understanding the structure and function of metalloenzymes and other metallobiomolecules. The specific techniques described include electron paramagnetic resonance (EPR) spectroscopy, and its derivative techniques, electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM). These latter two techniques directly probe the nuclear environment of the paramagnetic active site, without interference from magnetically active nuclei that are not involved in the active site. Magnetic circular dichroism (MCD), which links magnetic resonance and oprical spectrospoy, is also described. The application of MCD to problems in metallobiochemistry is growing rapidly, yet uhere are few sources that clearly describe this complex technique. This volume provides several approaches involving MCD. Finally, this book also includes a wide variety of biological systems including heme proteins, iron-sulfur proteins, molybdopterins, manganese proteins, and copper proteins, as well as non-protein biological molecules, such as ribozymes.

Table of Contents

Preface	xi
Paramagnetic Resonance of Metallobiomolecules: Introduction and Overview	1	(15)
Joshua Telser
Electron Paramagnetic Resonance and Related Techniques
Electron Spin Echo Methods: A Tutorial	16	(39)
R. David Britt
The Past, Present, and Future of Orientation-Selected Endor Analysis: Solving the Challenges of Dipolar-Coupled Nuclei	55	(27)
Peter E. Doan
Effects of Electron Spin Delocalization and Non-Collinearity of Interaction Terms in EPR Triplet Powder Patterns	82	(15)
Steven O. Mansoorabadi
George H. Reed
EPR Characterization of the Heme Oxygenase Reaction Intermediates and Its Implication for the Catalytic Mechanism	97	(16)
Masao Ikeda-Saito
Hiroshi Fujii
Paramagnetic Resonance in Mechanistic Studies of Fe-S/Radical Enzymes	113	(15)
Joan B. Broderick
Charles Walsby
William E. Broderick
Carsten Krebs
Wei Hong
Danilo Ortillo
Jennifer Cheek
Boi Hanh Huynh
Brian M. Hoffman
EPR and ENDOR Studies of [NiFe] Hydrogenase: Contributions to Understanding the Mechanism of Biological Hydrogen Conversion	128	(22)
Wolfgang Lubitz
Marc Brecht
Stefanie Foerster
Maurice van Gastel
Matthias Stein
Q-Band ENDOR Studies of the Nitrogenase MoFe Protein under Turnover Conditions: Substrate--Inhibitor-Binding to and Metal--Ion Valencies of the FeMo-Cofactor	150	(29)
Hong-In Lee
Linda M. Cameron
Jason Christiansen
Patricia D. Christie
Robert C. Pollock
Rutian Song
Morten Sorlie
W. H. Orme-Johnson
Dennis R. Dean
Brian J. Hales
Brian M. Hoffman
Variable Frequency Pulsed EPR Studies of Molybdenum Enzymes: Structure of Molybdenum Enzymes	179	(14)
John H. Enemark
Andrei V. Astashkin
Arnold M. Raitsimring
Mn2+ Sites Investigated by Advanced EPR Techniques: In-Depth Study of Mn2+ Ion-Binding Sites in the Hammerhead Ribozyme	193	(21)
Matthew Vogt
Victoria J. DeRose
Nuclear Paramagnetic Resonance
Structure and Dynamics of Paramagnetic Proteins by NMR	214	(13)
Milka Kostic
Thomas C. Pochapsky
An Approach to NMR Treatment of Structural Perturbations in Paramagnetic Proteins too Big for Solution Structure Determination	227	(17)
Judith M. Nocek
Kai Huang
Brian M. Hoffman
Proton NMR Characterization of Recombinant Ferric Heme Domains of the Oxygen Sensors FixL and Dos: Evidence for Protein Heterogeneity	244	(14)
James D. Satterlee
Christine M. Suquet
Marina I. Savenkova
Chenyang Lian
NMR Investigation of the Iron-Sulfur Cluster Environment in the Hyperthermostable Ferredoxin from Pyrococcus furiosus	258	(14)
Luigi Calzolai
Halvard Haarklau
Philip S. Brereton
Michael W. W. Adams
Gerd N. La Mar
Electronic Isomerism in Oxidized High-Potential Iron-Sulfur Proteins Revisited	272	(15)
Ivano Bertini
Francesco Capozzi
Claudio Luchinat
Paramagnetic NMR of Electron Transfer Copper Proteins	287	(17)
Claudio O. Fernandez
Alejandro J. Vila
Applications of Paramagnetic NMR Spectroscopy for Monitoring Transition Metal Complex Stoichiometry and Speciation	304	(24)
Debbie C. Crans
Luqin Yang
Ernestas Gaidamauskas
Raza Khan
Wenzheng Jin
Ursula Simonis
Magnetic Circular Dichroism and Related Techniques
Variable-Temperature Variable-Field Magnetic Circular Dichroism Combined with Electron Paramagnetic Resonance: Polarizations of Electronic Transitions in Solution	328	(12)
Edward I. Solomon
Mindy I. Davis
Frank Neese
Monita Y. M. Pau
Magnetic Circular Dichroism Spectroscopy of Pyranopterin Molybdenum Enzymes	340	(18)
Martin L. Kirk
X-ray Magnetic Circular Dichroism: A Primer for Chemists	358	(47)
Stephen P. Cramer
Indexes
Author Index	405	(2)
Subject Index	407