Peptide Arrays on Membrane Supports

by Koch, Joachim; Mahler, Michael

• ISBN13: 9783540425328
• ISBN10: 3540425322
• Format: Paperback
• Copyright: 2002-04-01
• Publisher: Springer Nature

Summary

Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding. The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.

Table of Contents

SPOT Synthesis - Scope of Applications	1	(22)
Ronald Frank
Jens Schneider-Mergener
Chemistry of Fmoc Peptide Synthesis on Membranes	23	(18)
Norbert Zander
Heinrich Gausepohl
Manual Peptide Synthesis	41	(14)
Gabriele Petersen
Automated Synthesis of Solid-Phase Bound Peptides	55	(14)
Heinrich Gausepohl
Christian Behn
Epitope Mapping of Antibodies with Solid-Phase Oligopeptides	69	(14)
Joachim Koch
Michael Mahler
Martin Bluthner
Protein-Protein Interactions	83	(14)
Matthew R. Groves
Irmgard Sinning
Analysis of Protein-DNA Interactions	97	(10)
Monika Reuter
Elisabeth Moncke-Buchner
Affinity Purification and Competition Assays Using Solid-Phase Oligopeptides	107	(16)
Michael Mahler
Martin Bluthner
Joachim Koch
Mutational Analysis and Structure Predictions	123	(18)
Martin Bluthner
Joachim Koch
Michael Mahler
Modification of Immobilized Peptides	141	(12)
Jochen Bodem
Martin Bluthner
Immobilized Peptides to Study Protein-Protein Interactions - Potential and Pitfalls	153	(12)
Rudiger Brauning
Michael Mahler
Barbara Hugle-Dorr
Martin Bluthner
Joachim Koch
Gabriele Petersen
Abbreviations	165	(2)
Subject Index	167