Peptide Nucleic Acids

by Nielsen, Peter E.

• ISBN13: 9780954523244
• ISBN10: 0954523245
• Edition: 2nd
• Format: Hardcover
• Copyright: 2004-03-12
• Publisher: Taylor & Franci

Summary

Peptide Nucleic Acids, Second Editionhas been extensively revised, updated, and enlarged to contain many new chapters covering the most recent topics and applications in this fast-moving field. The book contains state-of-the-art protocols and applications on all aspects of peptide nucleic acids. Concepts are clearly explained with each chapter containing concise background information. Written by leading experts in the field, the book is an invaluable and complete reference work on this novel and exciting area.

Table of Contents

Contents	iii
Contributors	v
Preface	vii
Chapter 1 An Introduction to PNA	1	(36)
Peter E. Nielsen and Michael Egholm
Chapter 2 PNA Synthesis by Boc Chemistry	37	(24)
Troels Koch
Chapter 3 Synthesis of PNA Oligomers by Fmoc Chemistry	61	(16)
Ralph Casale, Ivar S. Jensen and Michael Egholm
Chapter 4 Thermodynamics of PNA Interactions	77	(30)
Tommi Ratilainen, Anders Holmén and Bengt Nordén
Chapter 5 Peptide Nucleic Acid Targeting of Double-Stranded DNA	107	(34)
Thomas Bentin, H. Jakob Larsen and Peter E. Nielsen
Chapter 6 PNA Beacons and Fluoroprobes	141	(12)
Bhaskar Datta, Miaomiao Wang and Bruce A. Armitage
Chapter 7 Vector Tagging via PNA	153	(22)
Alex G. Rebuffat, Alessio G. Bernasconi and Brigitte M. Frey
Chapter 8 PCR Clamping	175	(12)
Henrik Ørum
Chapter 9 PNA Directed Genome Rare Cutting: New Developments	187	(20)
Maxim D. Frank-Kamenetskii and Vadim V. Demidov
Chapter 10 PNA Openers for Duplex DNA: Basic Facts, Fine Tuning and Emerging Applications	207	(20)
Vadim V. Demidov
Chapter 11 Combined Use of PNA and DNA for Fluorescence in Situ Hybridization (FISH)	227	(34)
Kirsten Vang Nielsen, Sven Müller, Tim Svenstrup Poulsen, Susanne Gabs and Andreas Schonau
Chapter 12 Production of PNA-Arrays for Nucleic Acid Detection	261	(20)
Anette Jacob, Ole Brandt, Simone Wurtz, Achim Stephan, Martina Schnolzer and Jorg D. Hoheisel
Chapter 13 Antisense in Eukaryotic Systems	281	(10)
David R. Corey
Chapter 14 Antisense Effects in Escherichia coli	291	(14)
Liam Good, Rikard Dryselius and Peter E. Nielsen
Appendix	305	(10)
Index	315